Gold nanoparticles affect the antioxidant status in selected normal human cells
Noami Daems,1 Sébastien Penninckx,2 Inge Nelissen,3 Karen Van Hoecke,4 Thomas Cardinaels,4,5 Sarah Baatout,1 Carine Michiels,6 Stéphane Lucas,2 An Aerts11Radiobiology Research Unit, Interdisciplinary Biosciences, Institute for Environment, Health and Safety, Belgian Nu...
| Main Authors: | , , , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Dove Medical Press
2019-07-01
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| Series: | International Journal of Nanomedicine |
| Subjects: | |
| Online Access: | https://www.dovepress.com/gold-nanoparticles-affect-the-antioxidant-status-in-selected-normal-hu-peer-reviewed-article-IJN |
| _version_ | 1831570235864383488 |
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| author | Daems N Penninckx S Nelissen I Van Hoecke K Cardinaels T Baatout S Michiels C Lucas S Aerts A |
| author_facet | Daems N Penninckx S Nelissen I Van Hoecke K Cardinaels T Baatout S Michiels C Lucas S Aerts A |
| author_sort | Daems N |
| collection | DOAJ |
| description | Noami Daems,1 Sébastien Penninckx,2 Inge Nelissen,3 Karen Van Hoecke,4 Thomas Cardinaels,4,5 Sarah Baatout,1 Carine Michiels,6 Stéphane Lucas,2 An Aerts11Radiobiology Research Unit, Interdisciplinary Biosciences, Institute for Environment, Health and Safety, Belgian Nuclear Research Centre (SCK.CEN), Mol, Belgium; 2Research Center for the Physics of Matter and Radiation-NARILIS, University of Namur, Namur, Belgium; 3Health Department, Flemish Institute For Technological Research (VITO), Mol, Belgium; 4Radiochemistry Expert Group, Institute for Nuclear Materials Science, Belgian Nuclear Research Centre (SCK.CEN), Mol, Belgium; 5Department of Chemistry, KU Leuven, Heverlee, Belgium; 6Unité de Recherche en Biologie Cellulaire-NARILIS, University of Namur, Namur, BelgiumPurpose: This study evaluates the cytotoxicity of AuNPs coated with polyallylamine (AuNPs-PAA) and conjugated or not to the epidermal growth factor receptor (EGFR)-targeting antibody Cetuximab (AuNPs-PAA-Ctxb) in normal human kidney (HK-2), liver (THLE-2) and microvascular endothelial (TIME) cells, and compares it with two cancer cell lines that are EGFR-overexpressing (A431) or EGFR-negative (MDA-MB-453).Results: Conjugation of Cetuximab to AuNPs-PAA increased the AuNPs-PAA-Ctxb interactions with cells, but reduced their cytotoxicity. TIME cells exhibited the strongest reduction in viability after exposure to AuNPs-PAA(±Ctxb), followed by THLE-2, MDA-MB-453, HK-2 and A431 cells. This cell type-dependent sensitivity was strongly correlated to the inhibition of thioredoxin reductase (TrxR) and glutathione reductase (GR), and to the depolarization of the mitochondrial membrane potential. Both are suggested to initiate apoptosis, which was indeed detected in a concentration- and time-dependent manner. The role of oxidative stress in AuNPs-PAA(±Ctxb)-induced cytotoxicity was demonstrated by co-incubation of the cells with N-acetyl L-cysteine (NAC), which significantly decreased apoptosis and mitochondrial membrane depolarization.Conclusion: This study helps to identify the cells and tissues that could be sensitive to AuNPs and deepens the understanding of the risks associated with the use of AuNPs in vivo.Keywords: cytotoxicity, EGFR, Cetuximab, oxidative stress |
| first_indexed | 2024-12-17T12:47:39Z |
| format | Article |
| id | doaj.art-20c81d3974c548d0b171ef40fafafd89 |
| institution | Directory Open Access Journal |
| issn | 1178-2013 |
| language | English |
| last_indexed | 2024-12-17T12:47:39Z |
| publishDate | 2019-07-01 |
| publisher | Dove Medical Press |
| record_format | Article |
| series | International Journal of Nanomedicine |
| spelling | doaj.art-20c81d3974c548d0b171ef40fafafd892022-12-21T21:47:41ZengDove Medical PressInternational Journal of Nanomedicine1178-20132019-07-01Volume 144991501546937Gold nanoparticles affect the antioxidant status in selected normal human cellsDaems NPenninckx SNelissen IVan Hoecke KCardinaels TBaatout SMichiels CLucas SAerts ANoami Daems,1 Sébastien Penninckx,2 Inge Nelissen,3 Karen Van Hoecke,4 Thomas Cardinaels,4,5 Sarah Baatout,1 Carine Michiels,6 Stéphane Lucas,2 An Aerts11Radiobiology Research Unit, Interdisciplinary Biosciences, Institute for Environment, Health and Safety, Belgian Nuclear Research Centre (SCK.CEN), Mol, Belgium; 2Research Center for the Physics of Matter and Radiation-NARILIS, University of Namur, Namur, Belgium; 3Health Department, Flemish Institute For Technological Research (VITO), Mol, Belgium; 4Radiochemistry Expert Group, Institute for Nuclear Materials Science, Belgian Nuclear Research Centre (SCK.CEN), Mol, Belgium; 5Department of Chemistry, KU Leuven, Heverlee, Belgium; 6Unité de Recherche en Biologie Cellulaire-NARILIS, University of Namur, Namur, BelgiumPurpose: This study evaluates the cytotoxicity of AuNPs coated with polyallylamine (AuNPs-PAA) and conjugated or not to the epidermal growth factor receptor (EGFR)-targeting antibody Cetuximab (AuNPs-PAA-Ctxb) in normal human kidney (HK-2), liver (THLE-2) and microvascular endothelial (TIME) cells, and compares it with two cancer cell lines that are EGFR-overexpressing (A431) or EGFR-negative (MDA-MB-453).Results: Conjugation of Cetuximab to AuNPs-PAA increased the AuNPs-PAA-Ctxb interactions with cells, but reduced their cytotoxicity. TIME cells exhibited the strongest reduction in viability after exposure to AuNPs-PAA(±Ctxb), followed by THLE-2, MDA-MB-453, HK-2 and A431 cells. This cell type-dependent sensitivity was strongly correlated to the inhibition of thioredoxin reductase (TrxR) and glutathione reductase (GR), and to the depolarization of the mitochondrial membrane potential. Both are suggested to initiate apoptosis, which was indeed detected in a concentration- and time-dependent manner. The role of oxidative stress in AuNPs-PAA(±Ctxb)-induced cytotoxicity was demonstrated by co-incubation of the cells with N-acetyl L-cysteine (NAC), which significantly decreased apoptosis and mitochondrial membrane depolarization.Conclusion: This study helps to identify the cells and tissues that could be sensitive to AuNPs and deepens the understanding of the risks associated with the use of AuNPs in vivo.Keywords: cytotoxicity, EGFR, Cetuximab, oxidative stresshttps://www.dovepress.com/gold-nanoparticles-affect-the-antioxidant-status-in-selected-normal-hu-peer-reviewed-article-IJNcytotoxicityEGFRCetuximaboxidative stress |
| spellingShingle | Daems N Penninckx S Nelissen I Van Hoecke K Cardinaels T Baatout S Michiels C Lucas S Aerts A Gold nanoparticles affect the antioxidant status in selected normal human cells International Journal of Nanomedicine cytotoxicity EGFR Cetuximab oxidative stress |
| title | Gold nanoparticles affect the antioxidant status in selected normal human cells |
| title_full | Gold nanoparticles affect the antioxidant status in selected normal human cells |
| title_fullStr | Gold nanoparticles affect the antioxidant status in selected normal human cells |
| title_full_unstemmed | Gold nanoparticles affect the antioxidant status in selected normal human cells |
| title_short | Gold nanoparticles affect the antioxidant status in selected normal human cells |
| title_sort | gold nanoparticles affect the antioxidant status in selected normal human cells |
| topic | cytotoxicity EGFR Cetuximab oxidative stress |
| url | https://www.dovepress.com/gold-nanoparticles-affect-the-antioxidant-status-in-selected-normal-hu-peer-reviewed-article-IJN |
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