Genome-Wide Identification and Expression Profiling of the <i>SRS</i> Gene Family in <i>Melilotus albus</i> Reveals Functions in Various Stress Conditions
The plant-specific <i>SHI</i>-related sequence (<i>SRS</i>) family of transcription factors plays a vital role in growth regulation, plant development, phytohormone biosynthesis, and stress response. However, the genome-wide identification and role in the abiotic stress-relat...
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MDPI AG
2022-11-01
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| Series: | Plants |
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| Online Access: | https://www.mdpi.com/2223-7747/11/22/3101 |
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| author | Biao Ma Lili Nian Noor ul Ain Xuelu Liu Yingbo Yang Xiaolin Zhu Fasih Ullah Haider Ying Lv Pengpeng Bai Xiaoning Zhang Quanxi Li Zixuan Mao Zongyang Xue |
| author_facet | Biao Ma Lili Nian Noor ul Ain Xuelu Liu Yingbo Yang Xiaolin Zhu Fasih Ullah Haider Ying Lv Pengpeng Bai Xiaoning Zhang Quanxi Li Zixuan Mao Zongyang Xue |
| author_sort | Biao Ma |
| collection | DOAJ |
| description | The plant-specific <i>SHI</i>-related sequence (<i>SRS</i>) family of transcription factors plays a vital role in growth regulation, plant development, phytohormone biosynthesis, and stress response. However, the genome-wide identification and role in the abiotic stress-related functions of the <i>SRS</i> gene family were not reported in white sweet clover (<i>Melilotus albus</i>). In this study, nine <i>M. albus SRS</i> genes (named <i>MaSRS01</i>-<i>MaSRS09</i>) were identified via a genome-wide search method. All nine genes were located on six out of eight chromosomes in the genome of <i>M. albus</i> and duplication analysis indicated eight segmentally duplicated genes in the <i>MaSRS</i> family. These <i>MaSRS</i> genes were classified into six groups based on their phylogenetic relationships. The gene structure and motif composition results indicated that <i>MaSRS</i> members in the same group contained analogous intron/exon and motif organizations. Further, promoter region analysis of <i>MaSRS</i> genes uncovered various growth, development, and stress-responsive <i>cis</i>-acting elements. Protein interaction networks showed that each gene has both functions of interacting with other genes and members within the family. Moreover, real-time quantitative PCR was also performed to verify the expression patterns of nine <i>MaSRS</i> genes in the leaves of <i>M. albus</i>. The results showed that nine <i>MaSRSs</i> were up- and down-regulated at different time points after various stress treatments, such as salinity, low-temperature, salicylic acid (SA), and methyl jasmonate (MeJA). This is the first systematic study of the <i>M. albus SRS</i> gene family, and it can serve as a strong foundation for further elucidation of the stress response and physiological improvement of the growth functions in <i>M. albus</i>. |
| first_indexed | 2024-03-09T18:03:41Z |
| format | Article |
| id | doaj.art-20c8b4ed95014e37b2a2e8d58912ba6e |
| institution | Directory Open Access Journal |
| issn | 2223-7747 |
| language | English |
| last_indexed | 2024-03-09T18:03:41Z |
| publishDate | 2022-11-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Plants |
| spelling | doaj.art-20c8b4ed95014e37b2a2e8d58912ba6e2023-11-24T09:39:39ZengMDPI AGPlants2223-77472022-11-011122310110.3390/plants11223101Genome-Wide Identification and Expression Profiling of the <i>SRS</i> Gene Family in <i>Melilotus albus</i> Reveals Functions in Various Stress ConditionsBiao Ma0Lili Nian1Noor ul Ain2Xuelu Liu3Yingbo Yang4Xiaolin Zhu5Fasih Ullah Haider6Ying Lv7Pengpeng Bai8Xiaoning Zhang9Quanxi Li10Zixuan Mao11Zongyang Xue12College of Resources and Environmental Sciences, Gansu Agricultural University, Lanzhou 730070, ChinaCollege of Forestry, Gansu Agricultural University, Lanzhou 730070, ChinaCentre of Genomics and Biotechnology, Fujian Agriculture and Forestry University, Jinshan, Fuzhou 350002, ChinaCollege of Resources and Environmental Sciences, Gansu Agricultural University, Lanzhou 730070, ChinaCollege of Resources and Environmental Sciences, Gansu Agricultural University, Lanzhou 730070, ChinaCollege of Agronomy, Gansu Agricultural University, Lanzhou 730070, ChinaKey Laboratory of Vegetation Restoration and Management of Degraded Ecosystems, South China Botanical Garden, Chinese Academy of Sciences, Guangzhou 510650, ChinaCollege of Resources and Environmental Sciences, Gansu Agricultural University, Lanzhou 730070, ChinaCollege of Resources and Environmental Sciences, Gansu Agricultural University, Lanzhou 730070, ChinaCollege of Forestry, Gansu Agricultural University, Lanzhou 730070, ChinaCollege of Resources and Environmental Sciences, Gansu Agricultural University, Lanzhou 730070, ChinaCollege of Resources and Environmental Sciences, Gansu Agricultural University, Lanzhou 730070, ChinaCollege of Resources and Environmental Sciences, Gansu Agricultural University, Lanzhou 730070, ChinaThe plant-specific <i>SHI</i>-related sequence (<i>SRS</i>) family of transcription factors plays a vital role in growth regulation, plant development, phytohormone biosynthesis, and stress response. However, the genome-wide identification and role in the abiotic stress-related functions of the <i>SRS</i> gene family were not reported in white sweet clover (<i>Melilotus albus</i>). In this study, nine <i>M. albus SRS</i> genes (named <i>MaSRS01</i>-<i>MaSRS09</i>) were identified via a genome-wide search method. All nine genes were located on six out of eight chromosomes in the genome of <i>M. albus</i> and duplication analysis indicated eight segmentally duplicated genes in the <i>MaSRS</i> family. These <i>MaSRS</i> genes were classified into six groups based on their phylogenetic relationships. The gene structure and motif composition results indicated that <i>MaSRS</i> members in the same group contained analogous intron/exon and motif organizations. Further, promoter region analysis of <i>MaSRS</i> genes uncovered various growth, development, and stress-responsive <i>cis</i>-acting elements. Protein interaction networks showed that each gene has both functions of interacting with other genes and members within the family. Moreover, real-time quantitative PCR was also performed to verify the expression patterns of nine <i>MaSRS</i> genes in the leaves of <i>M. albus</i>. The results showed that nine <i>MaSRSs</i> were up- and down-regulated at different time points after various stress treatments, such as salinity, low-temperature, salicylic acid (SA), and methyl jasmonate (MeJA). This is the first systematic study of the <i>M. albus SRS</i> gene family, and it can serve as a strong foundation for further elucidation of the stress response and physiological improvement of the growth functions in <i>M. albus</i>.https://www.mdpi.com/2223-7747/11/22/3101<i>MaSRS</i>gene family<i>Melilotus albus</i>gene expression analysis |
| spellingShingle | Biao Ma Lili Nian Noor ul Ain Xuelu Liu Yingbo Yang Xiaolin Zhu Fasih Ullah Haider Ying Lv Pengpeng Bai Xiaoning Zhang Quanxi Li Zixuan Mao Zongyang Xue Genome-Wide Identification and Expression Profiling of the <i>SRS</i> Gene Family in <i>Melilotus albus</i> Reveals Functions in Various Stress Conditions Plants <i>MaSRS</i> gene family <i>Melilotus albus</i> gene expression analysis |
| title | Genome-Wide Identification and Expression Profiling of the <i>SRS</i> Gene Family in <i>Melilotus albus</i> Reveals Functions in Various Stress Conditions |
| title_full | Genome-Wide Identification and Expression Profiling of the <i>SRS</i> Gene Family in <i>Melilotus albus</i> Reveals Functions in Various Stress Conditions |
| title_fullStr | Genome-Wide Identification and Expression Profiling of the <i>SRS</i> Gene Family in <i>Melilotus albus</i> Reveals Functions in Various Stress Conditions |
| title_full_unstemmed | Genome-Wide Identification and Expression Profiling of the <i>SRS</i> Gene Family in <i>Melilotus albus</i> Reveals Functions in Various Stress Conditions |
| title_short | Genome-Wide Identification and Expression Profiling of the <i>SRS</i> Gene Family in <i>Melilotus albus</i> Reveals Functions in Various Stress Conditions |
| title_sort | genome wide identification and expression profiling of the i srs i gene family in i melilotus albus i reveals functions in various stress conditions |
| topic | <i>MaSRS</i> gene family <i>Melilotus albus</i> gene expression analysis |
| url | https://www.mdpi.com/2223-7747/11/22/3101 |
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