Genistein, daidzein, and resveratrols stimulate PGC-1β-mediated gene expression
PGC-1β is a transcriptional co-activator of nuclear receptors such as the estrogen receptor-related receptor (ERR). Transgenic overexpression of PGC-1β in mice increases energy expenditure and suppresses high-fat diet-induced obesity. In this study, we screened various food-derived and natural compo...
| Main Authors: | , , , , , , |
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| Format: | Article |
| Language: | English |
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Elsevier
2019-03-01
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| Series: | Biochemistry and Biophysics Reports |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S2405580818302097 |
| _version_ | 1818244169307521024 |
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| author | Ran Uchitomi Shiho Nakai Rintaro Matsuda Takumi Onishi Shinji Miura Yukino Hatazawa Yasutomi Kamei |
| author_facet | Ran Uchitomi Shiho Nakai Rintaro Matsuda Takumi Onishi Shinji Miura Yukino Hatazawa Yasutomi Kamei |
| author_sort | Ran Uchitomi |
| collection | DOAJ |
| description | PGC-1β is a transcriptional co-activator of nuclear receptors such as the estrogen receptor-related receptor (ERR). Transgenic overexpression of PGC-1β in mice increases energy expenditure and suppresses high-fat diet-induced obesity. In this study, we screened various food-derived and natural compounds using a reporter assay system to measure the transcriptional activity of PGC-1β. Soy-derived isoflavones, genistein and daidzein, and several resveratrols activated PGC-1β. Genistein, daidzein, and trans-oxyresveratrol activated ERR-responsive element-mediated reporter activity in the presence of PGC-1β. Stable overexpression of PGC-1β in C2C12 myoblasts increased the expression of medium-chain acyl-CoA dehydrogenase (MCAD), an important enzyme in fatty acid β-oxidation. Genistein and daidzein increased MCAD mRNA levels and mitochondrial content in PGC-1β-expressing C2C12 cells. These compounds activated ERR/PGC-1β complex-mediated gene expression, and our findings may be a practical foundation for developing functional foods targeting obesity. Keywords: PGC-1β, Muscle cells, Isoflavone, ERR, Mitochondria |
| first_indexed | 2024-12-12T14:12:45Z |
| format | Article |
| id | doaj.art-20cae27ad38a44deb61e6d5b00a725d0 |
| institution | Directory Open Access Journal |
| issn | 2405-5808 |
| language | English |
| last_indexed | 2024-12-12T14:12:45Z |
| publishDate | 2019-03-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Biochemistry and Biophysics Reports |
| spelling | doaj.art-20cae27ad38a44deb61e6d5b00a725d02022-12-22T00:22:00ZengElsevierBiochemistry and Biophysics Reports2405-58082019-03-01175155Genistein, daidzein, and resveratrols stimulate PGC-1β-mediated gene expressionRan Uchitomi0Shiho Nakai1Rintaro Matsuda2Takumi Onishi3Shinji Miura4Yukino Hatazawa5Yasutomi Kamei6Graduate School of Life and Environmental Sciences, Kyoto Prefectural University, Kyoto, JapanGraduate School of Life and Environmental Sciences, Kyoto Prefectural University, Kyoto, JapanGraduate School of Life and Environmental Sciences, Kyoto Prefectural University, Kyoto, JapanGraduate School of Life and Environmental Sciences, Kyoto Prefectural University, Kyoto, JapanGraduate School of Nutritional and Environmental Sciences, University of Shizuoka, Shizuoka, JapanGraduate School of Life and Environmental Sciences, Kyoto Prefectural University, Kyoto, JapanGraduate School of Life and Environmental Sciences, Kyoto Prefectural University, Kyoto, Japan; Correspondence to: Laboratory of Molecular Nutrition, Graduate School of Life and Environmental Sciences, Kyoto Prefectural University, Kyoto 606-8522 Japan.PGC-1β is a transcriptional co-activator of nuclear receptors such as the estrogen receptor-related receptor (ERR). Transgenic overexpression of PGC-1β in mice increases energy expenditure and suppresses high-fat diet-induced obesity. In this study, we screened various food-derived and natural compounds using a reporter assay system to measure the transcriptional activity of PGC-1β. Soy-derived isoflavones, genistein and daidzein, and several resveratrols activated PGC-1β. Genistein, daidzein, and trans-oxyresveratrol activated ERR-responsive element-mediated reporter activity in the presence of PGC-1β. Stable overexpression of PGC-1β in C2C12 myoblasts increased the expression of medium-chain acyl-CoA dehydrogenase (MCAD), an important enzyme in fatty acid β-oxidation. Genistein and daidzein increased MCAD mRNA levels and mitochondrial content in PGC-1β-expressing C2C12 cells. These compounds activated ERR/PGC-1β complex-mediated gene expression, and our findings may be a practical foundation for developing functional foods targeting obesity. Keywords: PGC-1β, Muscle cells, Isoflavone, ERR, Mitochondriahttp://www.sciencedirect.com/science/article/pii/S2405580818302097 |
| spellingShingle | Ran Uchitomi Shiho Nakai Rintaro Matsuda Takumi Onishi Shinji Miura Yukino Hatazawa Yasutomi Kamei Genistein, daidzein, and resveratrols stimulate PGC-1β-mediated gene expression Biochemistry and Biophysics Reports |
| title | Genistein, daidzein, and resveratrols stimulate PGC-1β-mediated gene expression |
| title_full | Genistein, daidzein, and resveratrols stimulate PGC-1β-mediated gene expression |
| title_fullStr | Genistein, daidzein, and resveratrols stimulate PGC-1β-mediated gene expression |
| title_full_unstemmed | Genistein, daidzein, and resveratrols stimulate PGC-1β-mediated gene expression |
| title_short | Genistein, daidzein, and resveratrols stimulate PGC-1β-mediated gene expression |
| title_sort | genistein daidzein and resveratrols stimulate pgc 1β mediated gene expression |
| url | http://www.sciencedirect.com/science/article/pii/S2405580818302097 |
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