Microfluidic-Based Nucleic Acid Amplification Systems in Microbiology
Rapid, sensitive, and selective bacterial detection is a hot topic, because the progress in this research area has had a broad range of applications. Novel and innovative strategies for detection and identification of bacterial nucleic acids are important for practical applications. Microfluidics is...
| Main Authors: | , , |
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| Format: | Article |
| Language: | English |
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MDPI AG
2019-06-01
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| Series: | Micromachines |
| Subjects: | |
| Online Access: | https://www.mdpi.com/2072-666X/10/6/408 |
| _version_ | 1828526311382974464 |
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| author | Lena Gorgannezhad Helen Stratton Nam-Trung Nguyen |
| author_facet | Lena Gorgannezhad Helen Stratton Nam-Trung Nguyen |
| author_sort | Lena Gorgannezhad |
| collection | DOAJ |
| description | Rapid, sensitive, and selective bacterial detection is a hot topic, because the progress in this research area has had a broad range of applications. Novel and innovative strategies for detection and identification of bacterial nucleic acids are important for practical applications. Microfluidics is an emerging technology that only requires small amounts of liquid samples. Microfluidic devices allow for rapid advances in microbiology, enabling access to methods of amplifying nucleic acid molecules and overcoming difficulties faced by conventional. In this review, we summarize the recent progress in microfluidics-based polymerase chain reaction devices for the detection of nucleic acid biomarkers. The paper also discusses the recent development of isothermal nucleic acid amplification and droplet-based microfluidics devices. We discuss recent microfluidic techniques for sample preparation prior to the amplification process. |
| first_indexed | 2024-12-11T21:21:11Z |
| format | Article |
| id | doaj.art-20caeabfe13642f4af2582329dfdd205 |
| institution | Directory Open Access Journal |
| issn | 2072-666X |
| language | English |
| last_indexed | 2024-12-11T21:21:11Z |
| publishDate | 2019-06-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Micromachines |
| spelling | doaj.art-20caeabfe13642f4af2582329dfdd2052022-12-22T00:50:27ZengMDPI AGMicromachines2072-666X2019-06-0110640810.3390/mi10060408mi10060408Microfluidic-Based Nucleic Acid Amplification Systems in MicrobiologyLena Gorgannezhad0Helen Stratton1Nam-Trung Nguyen2Queensland Micro- and Nanotechnology Centre, Nathan Campus, Griffith University, 170 Kessels Road, Brisbane QLD 4111, AustraliaSchool of Environment and Science, Nathan Campus, Griffith University, 170 Kessels Road, Brisbane QLD 4111, AustraliaQueensland Micro- and Nanotechnology Centre, Nathan Campus, Griffith University, 170 Kessels Road, Brisbane QLD 4111, AustraliaRapid, sensitive, and selective bacterial detection is a hot topic, because the progress in this research area has had a broad range of applications. Novel and innovative strategies for detection and identification of bacterial nucleic acids are important for practical applications. Microfluidics is an emerging technology that only requires small amounts of liquid samples. Microfluidic devices allow for rapid advances in microbiology, enabling access to methods of amplifying nucleic acid molecules and overcoming difficulties faced by conventional. In this review, we summarize the recent progress in microfluidics-based polymerase chain reaction devices for the detection of nucleic acid biomarkers. The paper also discusses the recent development of isothermal nucleic acid amplification and droplet-based microfluidics devices. We discuss recent microfluidic techniques for sample preparation prior to the amplification process.https://www.mdpi.com/2072-666X/10/6/408bacterial nucleic acidmicrofluidicPCRLAMPdroplet |
| spellingShingle | Lena Gorgannezhad Helen Stratton Nam-Trung Nguyen Microfluidic-Based Nucleic Acid Amplification Systems in Microbiology Micromachines bacterial nucleic acid microfluidic PCR LAMP droplet |
| title | Microfluidic-Based Nucleic Acid Amplification Systems in Microbiology |
| title_full | Microfluidic-Based Nucleic Acid Amplification Systems in Microbiology |
| title_fullStr | Microfluidic-Based Nucleic Acid Amplification Systems in Microbiology |
| title_full_unstemmed | Microfluidic-Based Nucleic Acid Amplification Systems in Microbiology |
| title_short | Microfluidic-Based Nucleic Acid Amplification Systems in Microbiology |
| title_sort | microfluidic based nucleic acid amplification systems in microbiology |
| topic | bacterial nucleic acid microfluidic PCR LAMP droplet |
| url | https://www.mdpi.com/2072-666X/10/6/408 |
| work_keys_str_mv | AT lenagorgannezhad microfluidicbasednucleicacidamplificationsystemsinmicrobiology AT helenstratton microfluidicbasednucleicacidamplificationsystemsinmicrobiology AT namtrungnguyen microfluidicbasednucleicacidamplificationsystemsinmicrobiology |