A Novel Non-Coding RNA CsiR Regulates the Ciprofloxacin Resistance in <i>Proteus vulgaris</i> by Interacting with <i>emrB</i> mRNA
Bacterial non-coding RNAs (ncRNAs) play important regulatory roles in various physiological metabolic pathways. In this study, a novel ncRNA CsiR (ciprofloxacin stress-induced ncRNA) involved in the regulation of ciprofloxacin resistance in the foodborne multidrug-resistant <i>Proteus vulgaris...
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MDPI AG
2021-09-01
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| Series: | International Journal of Molecular Sciences |
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| Online Access: | https://www.mdpi.com/1422-0067/22/19/10627 |
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| author | Hongyang Zhang Tongzhen Song Chuhan Qin Haijin Xu Mingqiang Qiao |
| author_facet | Hongyang Zhang Tongzhen Song Chuhan Qin Haijin Xu Mingqiang Qiao |
| author_sort | Hongyang Zhang |
| collection | DOAJ |
| description | Bacterial non-coding RNAs (ncRNAs) play important regulatory roles in various physiological metabolic pathways. In this study, a novel ncRNA CsiR (ciprofloxacin stress-induced ncRNA) involved in the regulation of ciprofloxacin resistance in the foodborne multidrug-resistant <i>Proteus vulgaris</i> (<i>P. vulgaris</i>) strain P3M was identified. The survival rate of the CsiR-deficient strain was higher than that of the wild-type strain P3M under the ciprofloxacin treatment condition, indicating that CsiR played a negative regulatory role, and its target gene <i>emrB</i> was identified through further target prediction, quantitative real-time PCR (qRT-PCR), and microscale thermophoresis (MST). Further studies showed that the interaction between CsiR and <i>emrB</i> mRNA affected the stability of the latter at the post-transcriptional level to a large degree, and ultimately affected the ciprofloxacin resistance of P3M. Notably, the base-pairing sites between CsiR and <i>emrB</i> mRNAs were highly conserved in other sequenced <i>P. vulgaris</i> strains, suggesting that this regulatory mechanism may be ubiquitous in this species. To the best of our knowledge, this is the first identification of a novel ncRNA involved in the regulation of ciprofloxacin resistance in <i>P. vulgaris</i> species, which lays a solid foundation for comprehensively expounding the antibiotic resistance mechanism of <i>P. vulgaris</i>. |
| first_indexed | 2024-03-10T06:58:36Z |
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| id | doaj.art-20ce188a84c1482e97eb2c7072089c0d |
| institution | Directory Open Access Journal |
| issn | 1661-6596 1422-0067 |
| language | English |
| last_indexed | 2024-03-10T06:58:36Z |
| publishDate | 2021-09-01 |
| publisher | MDPI AG |
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| series | International Journal of Molecular Sciences |
| spelling | doaj.art-20ce188a84c1482e97eb2c7072089c0d2023-11-22T16:12:51ZengMDPI AGInternational Journal of Molecular Sciences1661-65961422-00672021-09-0122191062710.3390/ijms221910627A Novel Non-Coding RNA CsiR Regulates the Ciprofloxacin Resistance in <i>Proteus vulgaris</i> by Interacting with <i>emrB</i> mRNAHongyang Zhang0Tongzhen Song1Chuhan Qin2Haijin Xu3Mingqiang Qiao4College of Biotechnology and Food Science, Tianjin University of Commerce, Tianjin 300134, ChinaThe Key Laboratory of Molecular Microbiology and Technology, Ministry of Education, College of Life Sciences, Nankai University, Tianjin 300071, ChinaThe Key Laboratory of Molecular Microbiology and Technology, Ministry of Education, College of Life Sciences, Nankai University, Tianjin 300071, ChinaThe Key Laboratory of Molecular Microbiology and Technology, Ministry of Education, College of Life Sciences, Nankai University, Tianjin 300071, ChinaThe Key Laboratory of Molecular Microbiology and Technology, Ministry of Education, College of Life Sciences, Nankai University, Tianjin 300071, ChinaBacterial non-coding RNAs (ncRNAs) play important regulatory roles in various physiological metabolic pathways. In this study, a novel ncRNA CsiR (ciprofloxacin stress-induced ncRNA) involved in the regulation of ciprofloxacin resistance in the foodborne multidrug-resistant <i>Proteus vulgaris</i> (<i>P. vulgaris</i>) strain P3M was identified. The survival rate of the CsiR-deficient strain was higher than that of the wild-type strain P3M under the ciprofloxacin treatment condition, indicating that CsiR played a negative regulatory role, and its target gene <i>emrB</i> was identified through further target prediction, quantitative real-time PCR (qRT-PCR), and microscale thermophoresis (MST). Further studies showed that the interaction between CsiR and <i>emrB</i> mRNA affected the stability of the latter at the post-transcriptional level to a large degree, and ultimately affected the ciprofloxacin resistance of P3M. Notably, the base-pairing sites between CsiR and <i>emrB</i> mRNAs were highly conserved in other sequenced <i>P. vulgaris</i> strains, suggesting that this regulatory mechanism may be ubiquitous in this species. To the best of our knowledge, this is the first identification of a novel ncRNA involved in the regulation of ciprofloxacin resistance in <i>P. vulgaris</i> species, which lays a solid foundation for comprehensively expounding the antibiotic resistance mechanism of <i>P. vulgaris</i>.https://www.mdpi.com/1422-0067/22/19/10627non-coding RNACsiR<i>emrB</i>ciprofloxacin resistance<i>Proteus vulgaris</i> |
| spellingShingle | Hongyang Zhang Tongzhen Song Chuhan Qin Haijin Xu Mingqiang Qiao A Novel Non-Coding RNA CsiR Regulates the Ciprofloxacin Resistance in <i>Proteus vulgaris</i> by Interacting with <i>emrB</i> mRNA International Journal of Molecular Sciences non-coding RNA CsiR <i>emrB</i> ciprofloxacin resistance <i>Proteus vulgaris</i> |
| title | A Novel Non-Coding RNA CsiR Regulates the Ciprofloxacin Resistance in <i>Proteus vulgaris</i> by Interacting with <i>emrB</i> mRNA |
| title_full | A Novel Non-Coding RNA CsiR Regulates the Ciprofloxacin Resistance in <i>Proteus vulgaris</i> by Interacting with <i>emrB</i> mRNA |
| title_fullStr | A Novel Non-Coding RNA CsiR Regulates the Ciprofloxacin Resistance in <i>Proteus vulgaris</i> by Interacting with <i>emrB</i> mRNA |
| title_full_unstemmed | A Novel Non-Coding RNA CsiR Regulates the Ciprofloxacin Resistance in <i>Proteus vulgaris</i> by Interacting with <i>emrB</i> mRNA |
| title_short | A Novel Non-Coding RNA CsiR Regulates the Ciprofloxacin Resistance in <i>Proteus vulgaris</i> by Interacting with <i>emrB</i> mRNA |
| title_sort | novel non coding rna csir regulates the ciprofloxacin resistance in i proteus vulgaris i by interacting with i emrb i mrna |
| topic | non-coding RNA CsiR <i>emrB</i> ciprofloxacin resistance <i>Proteus vulgaris</i> |
| url | https://www.mdpi.com/1422-0067/22/19/10627 |
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