Microclonal propagation of elite industrial grape cultivars (<i>Vitis vinifera</i> L.)

Background. The grape is one of the most economically significant berry crops: its cultivation area covers over 6.7 million hectares. Due to certain biological characteristics of grapes, such as a long juvenile period (5–8 years), a high degree of genomic heterozygosity, and the frequently encounter...

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Main Author: A. Yu. Fizikova
Format: Article
Language:English
Published: N.I. Vavilov All-Russian Institute of Plant Genetic Resources 2024-01-01
Series:Труды по прикладной ботанике, генетике и селекции
Subjects:
Online Access:https://elpub.vir.nw.ru/jour/article/view/1744
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author A. Yu. Fizikova
author_facet A. Yu. Fizikova
author_sort A. Yu. Fizikova
collection DOAJ
description Background. The grape is one of the most economically significant berry crops: its cultivation area covers over 6.7 million hectares. Due to certain biological characteristics of grapes, such as a long juvenile period (5–8 years), a high degree of genomic heterozygosity, and the frequently encountered phenomenon of inbred depression, when homozygotization during hybridization leads to a loss of viability and production characteristics of the cultivar, vegetative propagation becomes the basis for grape reproduction and industrial cultivation. Microclonal propagation is the foundation for rejuvenation and revitalization of modern vineyards. Developing approaches for microclonal propagation of elite industrial grape cultivars remains a relevant task for the modern wine industry.Materials and methods. The industrial grape cultivars ‘Malbec’, ‘Merlot’, ‘Chardonnay’, and ‘Riesling’ from the field collection of the All-Russian National Research institute of Viticulture and Winemaking “Magarach” were used for the work.Results. This research succeeded in devising a universal, one-stage protocol for the microclonal propagation of elite industrial grape cultivars, such as ‘Merlot’, ‘Chardonnay’, ‘Malbec’, and ‘Riesling’, making the production of plants ready for adaptation into the soil (ex vitro) within 1 months after rooting.Conclusion. The use of the microclonal propagation protocol developed in this study for industrial grape cultivars will reduce labor costs and shorten the time required to obtain a plant ready for adaptation to open ground two to three times.
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spelling doaj.art-20cfed853b234ef5a5513fa6a5b5cf392024-01-22T19:30:15ZengN.I. Vavilov All-Russian Institute of Plant Genetic ResourcesТруды по прикладной ботанике, генетике и селекции2227-88342619-09822024-01-01184422223110.30901/2227-8834-2023-4-222-231763Microclonal propagation of elite industrial grape cultivars (<i>Vitis vinifera</i> L.)A. Yu. Fizikova0Sirius University of Science and Technology, Research Center of Genetics and Life SciencesBackground. The grape is one of the most economically significant berry crops: its cultivation area covers over 6.7 million hectares. Due to certain biological characteristics of grapes, such as a long juvenile period (5–8 years), a high degree of genomic heterozygosity, and the frequently encountered phenomenon of inbred depression, when homozygotization during hybridization leads to a loss of viability and production characteristics of the cultivar, vegetative propagation becomes the basis for grape reproduction and industrial cultivation. Microclonal propagation is the foundation for rejuvenation and revitalization of modern vineyards. Developing approaches for microclonal propagation of elite industrial grape cultivars remains a relevant task for the modern wine industry.Materials and methods. The industrial grape cultivars ‘Malbec’, ‘Merlot’, ‘Chardonnay’, and ‘Riesling’ from the field collection of the All-Russian National Research institute of Viticulture and Winemaking “Magarach” were used for the work.Results. This research succeeded in devising a universal, one-stage protocol for the microclonal propagation of elite industrial grape cultivars, such as ‘Merlot’, ‘Chardonnay’, ‘Malbec’, and ‘Riesling’, making the production of plants ready for adaptation into the soil (ex vitro) within 1 months after rooting.Conclusion. The use of the microclonal propagation protocol developed in this study for industrial grape cultivars will reduce labor costs and shorten the time required to obtain a plant ready for adaptation to open ground two to three times.https://elpub.vir.nw.ru/jour/article/view/1744medium<i>in vitro</i>universal protocol‘merlot’‘chardonnay’‘malbec’‘riesling’
spellingShingle A. Yu. Fizikova
Microclonal propagation of elite industrial grape cultivars (<i>Vitis vinifera</i> L.)
Труды по прикладной ботанике, генетике и селекции
medium
<i>in vitro</i>
universal protocol
‘merlot’
‘chardonnay’
‘malbec’
‘riesling’
title Microclonal propagation of elite industrial grape cultivars (<i>Vitis vinifera</i> L.)
title_full Microclonal propagation of elite industrial grape cultivars (<i>Vitis vinifera</i> L.)
title_fullStr Microclonal propagation of elite industrial grape cultivars (<i>Vitis vinifera</i> L.)
title_full_unstemmed Microclonal propagation of elite industrial grape cultivars (<i>Vitis vinifera</i> L.)
title_short Microclonal propagation of elite industrial grape cultivars (<i>Vitis vinifera</i> L.)
title_sort microclonal propagation of elite industrial grape cultivars i vitis vinifera i l
topic medium
<i>in vitro</i>
universal protocol
‘merlot’
‘chardonnay’
‘malbec’
‘riesling’
url https://elpub.vir.nw.ru/jour/article/view/1744
work_keys_str_mv AT ayufizikova microclonalpropagationofeliteindustrialgrapecultivarsivitisviniferail