High-resolution two-photon fluorescence microscope imaging of nanodiamonds containing NV color centers
Fluorescent nanodiamonds containing nitrogen-vacancy (NV) color centers play an important role in nanoscale sensing for their unique properties, including extraordinary photon stability, bio-compatibility, and chemical inertness at room temperature. Two-photon fluorescence imaging of fluorescent nan...
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| Format: | Article |
| Language: | English |
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Elsevier
2022-09-01
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| Series: | Results in Physics |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2211379722005150 |
| _version_ | 1828357775497887744 |
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| author | Zhiping Ju Mengting He Junjie Lin Jing Zhu Botao Wu E Wu |
| author_facet | Zhiping Ju Mengting He Junjie Lin Jing Zhu Botao Wu E Wu |
| author_sort | Zhiping Ju |
| collection | DOAJ |
| description | Fluorescent nanodiamonds containing nitrogen-vacancy (NV) color centers play an important role in nanoscale sensing for their unique properties, including extraordinary photon stability, bio-compatibility, and chemical inertness at room temperature. Two-photon fluorescence imaging of fluorescent nanodiamonds (FNDs) has been studied with a picosecond pulsed laser operating at 1032 nm. We obtained an imaging resolution of 318 nm at nearly a third of the excitation wavelength with 70 nm FNDs. In this case, the resolution was improved by 2.2-fold, compared with the diffraction-limited resolution of the near-infrared (NIR) single-photon confocal microscopic imaging. The axial resolution of the imaging is about 750 nm, which is about 1.4 times higher than the Rayleigh length under the same excitation. The fluorescence spectra of visible and near-infrared excitation of FNDs are similar, suggesting that the energy levels involved in the two processes are identical. With the unique advantages of high spatial resolution, high signal-to-noise ratio (SNR), and infrared window matching, the study of two-photon excitation microscope imaging of FNDs is conducive to its broader application in biology, physics, and materials sciences. |
| first_indexed | 2024-04-14T03:19:44Z |
| format | Article |
| id | doaj.art-20d1594dfb6a403a85d9e63d93bb31a7 |
| institution | Directory Open Access Journal |
| issn | 2211-3797 |
| language | English |
| last_indexed | 2024-04-14T03:19:44Z |
| publishDate | 2022-09-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Results in Physics |
| spelling | doaj.art-20d1594dfb6a403a85d9e63d93bb31a72022-12-22T02:15:20ZengElsevierResults in Physics2211-37972022-09-0140105874High-resolution two-photon fluorescence microscope imaging of nanodiamonds containing NV color centersZhiping Ju0Mengting He1Junjie Lin2Jing Zhu3Botao Wu4E Wu5State Key Laboratory of Precision Spectroscopy, East China Normal University, Shanghai 200062, People’s Republic of ChinaState Key Laboratory of Precision Spectroscopy, East China Normal University, Shanghai 200062, People’s Republic of ChinaState Key Laboratory of Precision Spectroscopy, East China Normal University, Shanghai 200062, People’s Republic of ChinaDepartment of Physics and Shanghai Key Laboratory for Magnetic Resonance, East China Normal University, Shanghai 200062, People’s Republic of ChinaState Key Laboratory of Precision Spectroscopy, East China Normal University, Shanghai 200062, People’s Republic of ChinaState Key Laboratory of Precision Spectroscopy, East China Normal University, Shanghai 200062, People’s Republic of China; Department of Physics and Shanghai Key Laboratory for Magnetic Resonance, East China Normal University, Shanghai 200062, People’s Republic of China; Chongqing Key Laboratory of Precision Optics, Chongqing Institute of East China Normal University, Chongqing 401120, People’s Republic of China; Collaborative Innovation Center of Extreme Optics, Shanxi University, Taiyuan, Shanxi 030006, People’s Republic of China; Corresponding author at: State Key Laboratory of Precision Spectroscopy, East China Normal University, Shanghai 200062, People’s Republic of China.Fluorescent nanodiamonds containing nitrogen-vacancy (NV) color centers play an important role in nanoscale sensing for their unique properties, including extraordinary photon stability, bio-compatibility, and chemical inertness at room temperature. Two-photon fluorescence imaging of fluorescent nanodiamonds (FNDs) has been studied with a picosecond pulsed laser operating at 1032 nm. We obtained an imaging resolution of 318 nm at nearly a third of the excitation wavelength with 70 nm FNDs. In this case, the resolution was improved by 2.2-fold, compared with the diffraction-limited resolution of the near-infrared (NIR) single-photon confocal microscopic imaging. The axial resolution of the imaging is about 750 nm, which is about 1.4 times higher than the Rayleigh length under the same excitation. The fluorescence spectra of visible and near-infrared excitation of FNDs are similar, suggesting that the energy levels involved in the two processes are identical. With the unique advantages of high spatial resolution, high signal-to-noise ratio (SNR), and infrared window matching, the study of two-photon excitation microscope imaging of FNDs is conducive to its broader application in biology, physics, and materials sciences.http://www.sciencedirect.com/science/article/pii/S2211379722005150Two-photon microscopeNitrogen-vacancy centersNanodiamond |
| spellingShingle | Zhiping Ju Mengting He Junjie Lin Jing Zhu Botao Wu E Wu High-resolution two-photon fluorescence microscope imaging of nanodiamonds containing NV color centers Results in Physics Two-photon microscope Nitrogen-vacancy centers Nanodiamond |
| title | High-resolution two-photon fluorescence microscope imaging of nanodiamonds containing NV color centers |
| title_full | High-resolution two-photon fluorescence microscope imaging of nanodiamonds containing NV color centers |
| title_fullStr | High-resolution two-photon fluorescence microscope imaging of nanodiamonds containing NV color centers |
| title_full_unstemmed | High-resolution two-photon fluorescence microscope imaging of nanodiamonds containing NV color centers |
| title_short | High-resolution two-photon fluorescence microscope imaging of nanodiamonds containing NV color centers |
| title_sort | high resolution two photon fluorescence microscope imaging of nanodiamonds containing nv color centers |
| topic | Two-photon microscope Nitrogen-vacancy centers Nanodiamond |
| url | http://www.sciencedirect.com/science/article/pii/S2211379722005150 |
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