Characterization of NFE2L1-616, an isoform of nuclear factor-erythroid-2 related transcription factor-1 that activates antioxidant response element-regulated genes
Abstract The NFE2L1 transcription factor (aka Nrf1) is a basic leucine zipper protein that performs a critical role in the cellular stress response pathway. Here, we characterized a novel variant of NFE2L1 referred to as NFE2L1-616. The transcript encoding NFE2L1-616 is derived from an intronic prom...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
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Nature Portfolio
2023-11-01
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| Series: | Scientific Reports |
| Online Access: | https://doi.org/10.1038/s41598-023-47055-2 |
| _version_ | 1797559814363021312 |
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| author | Daniel V. Ho Kaylen G. Suryajaya Kaitlyn Manh Amanda N. Duong Jefferson Y. Chan |
| author_facet | Daniel V. Ho Kaylen G. Suryajaya Kaitlyn Manh Amanda N. Duong Jefferson Y. Chan |
| author_sort | Daniel V. Ho |
| collection | DOAJ |
| description | Abstract The NFE2L1 transcription factor (aka Nrf1) is a basic leucine zipper protein that performs a critical role in the cellular stress response pathway. Here, we characterized a novel variant of NFE2L1 referred to as NFE2L1-616. The transcript encoding NFE2L1-616 is derived from an intronic promoter, and it has a distinct first exon than other reported full-length NFE2L1 isoforms. The NFE2L1-616 protein constitutively localizes in the nucleus as it lacks the N-terminal amino acid residues that targets other full-length NFE2L1 isoforms to the endoplasmic reticulum. The expression level of NFE2L1-616 is lower than other NFE2L1 isoforms. It is widely expressed across different cell lines and tissues that were examined. NFE2L1-616 showed strong transcriptional activity driving luciferase reporter expression from a promoter containing antioxidant response element. Together, the results suggest that NFE2L1-616 variant can function as a positive regulator in the transcriptional regulation of NFE2L1 responsive genes. |
| first_indexed | 2024-03-10T17:50:37Z |
| format | Article |
| id | doaj.art-20d3dc802ade4991afe65c95e9fb180b |
| institution | Directory Open Access Journal |
| issn | 2045-2322 |
| language | English |
| last_indexed | 2024-03-10T17:50:37Z |
| publishDate | 2023-11-01 |
| publisher | Nature Portfolio |
| record_format | Article |
| series | Scientific Reports |
| spelling | doaj.art-20d3dc802ade4991afe65c95e9fb180b2023-11-20T09:21:15ZengNature PortfolioScientific Reports2045-23222023-11-0113111210.1038/s41598-023-47055-2Characterization of NFE2L1-616, an isoform of nuclear factor-erythroid-2 related transcription factor-1 that activates antioxidant response element-regulated genesDaniel V. Ho0Kaylen G. Suryajaya1Kaitlyn Manh2Amanda N. Duong3Jefferson Y. Chan4Department of Laboratory Medicine and Pathology, University of California, IrvineDepartment of Laboratory Medicine and Pathology, University of California, IrvineDepartment of Laboratory Medicine and Pathology, University of California, IrvineDepartment of Laboratory Medicine and Pathology, University of California, IrvineDepartment of Laboratory Medicine and Pathology, University of California, IrvineAbstract The NFE2L1 transcription factor (aka Nrf1) is a basic leucine zipper protein that performs a critical role in the cellular stress response pathway. Here, we characterized a novel variant of NFE2L1 referred to as NFE2L1-616. The transcript encoding NFE2L1-616 is derived from an intronic promoter, and it has a distinct first exon than other reported full-length NFE2L1 isoforms. The NFE2L1-616 protein constitutively localizes in the nucleus as it lacks the N-terminal amino acid residues that targets other full-length NFE2L1 isoforms to the endoplasmic reticulum. The expression level of NFE2L1-616 is lower than other NFE2L1 isoforms. It is widely expressed across different cell lines and tissues that were examined. NFE2L1-616 showed strong transcriptional activity driving luciferase reporter expression from a promoter containing antioxidant response element. Together, the results suggest that NFE2L1-616 variant can function as a positive regulator in the transcriptional regulation of NFE2L1 responsive genes.https://doi.org/10.1038/s41598-023-47055-2 |
| spellingShingle | Daniel V. Ho Kaylen G. Suryajaya Kaitlyn Manh Amanda N. Duong Jefferson Y. Chan Characterization of NFE2L1-616, an isoform of nuclear factor-erythroid-2 related transcription factor-1 that activates antioxidant response element-regulated genes Scientific Reports |
| title | Characterization of NFE2L1-616, an isoform of nuclear factor-erythroid-2 related transcription factor-1 that activates antioxidant response element-regulated genes |
| title_full | Characterization of NFE2L1-616, an isoform of nuclear factor-erythroid-2 related transcription factor-1 that activates antioxidant response element-regulated genes |
| title_fullStr | Characterization of NFE2L1-616, an isoform of nuclear factor-erythroid-2 related transcription factor-1 that activates antioxidant response element-regulated genes |
| title_full_unstemmed | Characterization of NFE2L1-616, an isoform of nuclear factor-erythroid-2 related transcription factor-1 that activates antioxidant response element-regulated genes |
| title_short | Characterization of NFE2L1-616, an isoform of nuclear factor-erythroid-2 related transcription factor-1 that activates antioxidant response element-regulated genes |
| title_sort | characterization of nfe2l1 616 an isoform of nuclear factor erythroid 2 related transcription factor 1 that activates antioxidant response element regulated genes |
| url | https://doi.org/10.1038/s41598-023-47055-2 |
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