Establishment and Characterization of Immortalized Minipig Neural Stem Cell Line
Despite the increasing importance of minipigs in biomedical research, there has been relatively little research concerning minipig-derived adult stem cells as a promising research tool that could be used to develop stem cell-based therapies. We first generated immortalized neural stem cells (iNSCs)...
| Main Authors: | , , , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
SAGE Publishing
2017-02-01
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| Series: | Cell Transplantation |
| Online Access: | https://doi.org/10.3727/096368916X692852 |
| _version_ | 1819071249196777472 |
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| author | Sung S. Choi Seung-Bin Yoon Sang-Rae Lee Sun-Uk Kim Young Joo Cha Daniel Lee Seung U. Kim Kyu-Tae Chang Ph.D. Hong J. Lee Ph.D. |
| author_facet | Sung S. Choi Seung-Bin Yoon Sang-Rae Lee Sun-Uk Kim Young Joo Cha Daniel Lee Seung U. Kim Kyu-Tae Chang Ph.D. Hong J. Lee Ph.D. |
| author_sort | Sung S. Choi |
| collection | DOAJ |
| description | Despite the increasing importance of minipigs in biomedical research, there has been relatively little research concerning minipig-derived adult stem cells as a promising research tool that could be used to develop stem cell-based therapies. We first generated immortalized neural stem cells (iNSCs) from primary minipig olfactory bulb cells (pmpOBCs) and defined the characteristics of the cell line. Primary neural cells were prepared from minipig neonate olfactory bulbs and immortalized by infection with retrovirus carrying the v-myc gene. The minipig iNSCs (mpiNSCs) had normal karyotypes and expressed NSC-specific markers, including nestin, vimentin, Musashi1, and SOX2, suggesting a similarity to human NSCs. On the basis of the global gene expression profiles from the microarray analysis, neurogenesis-associated transcript levels were predominantly altered in mpiNSCs compared with pmpOBCs. These findings increase our understanding of minipig stem cells and contribute to the utility of mpiNSCs as resources for immortalized stem cell experiments. |
| first_indexed | 2024-12-21T17:18:50Z |
| format | Article |
| id | doaj.art-20d4ad3fffe64fd4aba07a89e81b724d |
| institution | Directory Open Access Journal |
| issn | 0963-6897 1555-3892 |
| language | English |
| last_indexed | 2024-12-21T17:18:50Z |
| publishDate | 2017-02-01 |
| publisher | SAGE Publishing |
| record_format | Article |
| series | Cell Transplantation |
| spelling | doaj.art-20d4ad3fffe64fd4aba07a89e81b724d2022-12-21T18:56:13ZengSAGE PublishingCell Transplantation0963-68971555-38922017-02-012610.3727/096368916X692852Establishment and Characterization of Immortalized Minipig Neural Stem Cell LineSung S. Choi0Seung-Bin Yoon1Sang-Rae Lee2Sun-Uk Kim3Young Joo Cha4Daniel Lee5Seung U. Kim6Kyu-Tae Chang Ph.D.7Hong J. Lee Ph.D.8Medical Research Institute, Chung-Ang University College of Medicine, Seoul, Republic of KoreaNational Primate Research Center, Korea Research Institute of Bioscience and Biotechnology, Chungju, Republic of KoreaNational Primate Research Center, Korea Research Institute of Bioscience and Biotechnology, Chungju, Republic of KoreaNational Primate Research Center, Korea Research Institute of Bioscience and Biotechnology, Chungju, Republic of KoreaDepartment of Laboratory Medicine, Chung-Ang University College of Medicine, Seoul, Republic of KoreaSeonbu High School, Ansan, Republic of KoreaDivision of Neurology, Department of Medicine, University of British Columbia, Vancouver, BC, CanadaNational Primate Research Center, Korea Research Institute of Bioscience and Biotechnology, Chungju, Republic of KoreaMedical Research Institute, Chung-Ang University College of Medicine, Seoul, Republic of KoreaDespite the increasing importance of minipigs in biomedical research, there has been relatively little research concerning minipig-derived adult stem cells as a promising research tool that could be used to develop stem cell-based therapies. We first generated immortalized neural stem cells (iNSCs) from primary minipig olfactory bulb cells (pmpOBCs) and defined the characteristics of the cell line. Primary neural cells were prepared from minipig neonate olfactory bulbs and immortalized by infection with retrovirus carrying the v-myc gene. The minipig iNSCs (mpiNSCs) had normal karyotypes and expressed NSC-specific markers, including nestin, vimentin, Musashi1, and SOX2, suggesting a similarity to human NSCs. On the basis of the global gene expression profiles from the microarray analysis, neurogenesis-associated transcript levels were predominantly altered in mpiNSCs compared with pmpOBCs. These findings increase our understanding of minipig stem cells and contribute to the utility of mpiNSCs as resources for immortalized stem cell experiments.https://doi.org/10.3727/096368916X692852 |
| spellingShingle | Sung S. Choi Seung-Bin Yoon Sang-Rae Lee Sun-Uk Kim Young Joo Cha Daniel Lee Seung U. Kim Kyu-Tae Chang Ph.D. Hong J. Lee Ph.D. Establishment and Characterization of Immortalized Minipig Neural Stem Cell Line Cell Transplantation |
| title | Establishment and Characterization of Immortalized Minipig Neural Stem Cell Line |
| title_full | Establishment and Characterization of Immortalized Minipig Neural Stem Cell Line |
| title_fullStr | Establishment and Characterization of Immortalized Minipig Neural Stem Cell Line |
| title_full_unstemmed | Establishment and Characterization of Immortalized Minipig Neural Stem Cell Line |
| title_short | Establishment and Characterization of Immortalized Minipig Neural Stem Cell Line |
| title_sort | establishment and characterization of immortalized minipig neural stem cell line |
| url | https://doi.org/10.3727/096368916X692852 |
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