Boundary Caps Give Rise to Neurogenic Stem Cells and Terminal Glia in the Skin
While neurogenic stem cells have been identified in rodent and human skin, their manipulation and further characterization are hampered by a lack of specific markers. Here, we perform genetic tracing of the progeny of boundary cap (BC) cells, a neural-crest-derived cell population localized at perip...
| Main Authors: | , , , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Elsevier
2015-08-01
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| Series: | Stem Cell Reports |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S2213671115001861 |
| _version_ | 1818893468883222528 |
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| author | Aurélie Gresset Fanny Coulpier Gaspard Gerschenfeld Alexandre Jourdon Graziella Matesic Laurence Richard Jean-Michel Vallat Patrick Charnay Piotr Topilko |
| author_facet | Aurélie Gresset Fanny Coulpier Gaspard Gerschenfeld Alexandre Jourdon Graziella Matesic Laurence Richard Jean-Michel Vallat Patrick Charnay Piotr Topilko |
| author_sort | Aurélie Gresset |
| collection | DOAJ |
| description | While neurogenic stem cells have been identified in rodent and human skin, their manipulation and further characterization are hampered by a lack of specific markers. Here, we perform genetic tracing of the progeny of boundary cap (BC) cells, a neural-crest-derived cell population localized at peripheral nerve entry/exit points. We show that BC derivatives migrate along peripheral nerves to reach the skin, where they give rise to terminal glia associated with dermal nerve endings. Dermal BC derivatives also include cells that self-renew in sphere culture and have broad in vitro differentiation potential. Upon transplantation into adult mouse dorsal root ganglia, skin BC derivatives efficiently differentiate into various types of mature sensory neurons. Together, this work establishes the embryonic origin, pathway of migration, and in vivo neurogenic potential of a major component of skin stem-like cells. It provides genetic tools to study and manipulate this population of high interest for medical applications. |
| first_indexed | 2024-12-19T18:13:05Z |
| format | Article |
| id | doaj.art-20d5cb516d2041bcb2c877d0adb3f851 |
| institution | Directory Open Access Journal |
| issn | 2213-6711 |
| language | English |
| last_indexed | 2024-12-19T18:13:05Z |
| publishDate | 2015-08-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Stem Cell Reports |
| spelling | doaj.art-20d5cb516d2041bcb2c877d0adb3f8512022-12-21T20:11:14ZengElsevierStem Cell Reports2213-67112015-08-015227829010.1016/j.stemcr.2015.06.005Boundary Caps Give Rise to Neurogenic Stem Cells and Terminal Glia in the SkinAurélie Gresset0Fanny Coulpier1Gaspard Gerschenfeld2Alexandre Jourdon3Graziella Matesic4Laurence Richard5Jean-Michel Vallat6Patrick Charnay7Piotr Topilko8Ecole Normale Supérieure, Institut de Biologie de l’ENS (IBENS), and INSERM U1024, and Centre National de la Recherche Scientifique (CNRS) UMR 8197, Paris 75005, FranceEcole Normale Supérieure, Institut de Biologie de l’ENS (IBENS), and INSERM U1024, and Centre National de la Recherche Scientifique (CNRS) UMR 8197, Paris 75005, FranceEcole Normale Supérieure, Institut de Biologie de l’ENS (IBENS), and INSERM U1024, and Centre National de la Recherche Scientifique (CNRS) UMR 8197, Paris 75005, FranceEcole Normale Supérieure, Institut de Biologie de l’ENS (IBENS), and INSERM U1024, and Centre National de la Recherche Scientifique (CNRS) UMR 8197, Paris 75005, FranceEcole Normale Supérieure, Institut de Biologie de l’ENS (IBENS), and INSERM U1024, and Centre National de la Recherche Scientifique (CNRS) UMR 8197, Paris 75005, FranceNational Reference Centre “Rare Peripheral Neuropathies” Department of Neurology, Centre Hospitalier Universitaire de Limoges, 87042 Limoges, FranceNational Reference Centre “Rare Peripheral Neuropathies” Department of Neurology, Centre Hospitalier Universitaire de Limoges, 87042 Limoges, FranceEcole Normale Supérieure, Institut de Biologie de l’ENS (IBENS), and INSERM U1024, and Centre National de la Recherche Scientifique (CNRS) UMR 8197, Paris 75005, FranceEcole Normale Supérieure, Institut de Biologie de l’ENS (IBENS), and INSERM U1024, and Centre National de la Recherche Scientifique (CNRS) UMR 8197, Paris 75005, FranceWhile neurogenic stem cells have been identified in rodent and human skin, their manipulation and further characterization are hampered by a lack of specific markers. Here, we perform genetic tracing of the progeny of boundary cap (BC) cells, a neural-crest-derived cell population localized at peripheral nerve entry/exit points. We show that BC derivatives migrate along peripheral nerves to reach the skin, where they give rise to terminal glia associated with dermal nerve endings. Dermal BC derivatives also include cells that self-renew in sphere culture and have broad in vitro differentiation potential. Upon transplantation into adult mouse dorsal root ganglia, skin BC derivatives efficiently differentiate into various types of mature sensory neurons. Together, this work establishes the embryonic origin, pathway of migration, and in vivo neurogenic potential of a major component of skin stem-like cells. It provides genetic tools to study and manipulate this population of high interest for medical applications.http://www.sciencedirect.com/science/article/pii/S2213671115001861 |
| spellingShingle | Aurélie Gresset Fanny Coulpier Gaspard Gerschenfeld Alexandre Jourdon Graziella Matesic Laurence Richard Jean-Michel Vallat Patrick Charnay Piotr Topilko Boundary Caps Give Rise to Neurogenic Stem Cells and Terminal Glia in the Skin Stem Cell Reports |
| title | Boundary Caps Give Rise to Neurogenic Stem Cells and Terminal Glia in the Skin |
| title_full | Boundary Caps Give Rise to Neurogenic Stem Cells and Terminal Glia in the Skin |
| title_fullStr | Boundary Caps Give Rise to Neurogenic Stem Cells and Terminal Glia in the Skin |
| title_full_unstemmed | Boundary Caps Give Rise to Neurogenic Stem Cells and Terminal Glia in the Skin |
| title_short | Boundary Caps Give Rise to Neurogenic Stem Cells and Terminal Glia in the Skin |
| title_sort | boundary caps give rise to neurogenic stem cells and terminal glia in the skin |
| url | http://www.sciencedirect.com/science/article/pii/S2213671115001861 |
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