Biosensor-Assisted Adaptive Laboratory Evolution for Violacein Production
Violacein is a naturally occurring purple pigment, widely used in cosmetics and has potent antibacterial and antiviral properties. Violacein can be produced from tryptophan, consequently sufficient tryptophan biosynthesis is the key to violacein production. However, the complicated biosynthetic path...
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| Format: | Article |
| Language: | English |
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MDPI AG
2021-06-01
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| Series: | International Journal of Molecular Sciences |
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| Online Access: | https://www.mdpi.com/1422-0067/22/12/6594 |
| _version_ | 1797529471912247296 |
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| author | Da-ae Gwon Joo Yeon Seok Gyoo Yeol Jung Jeong Wook Lee |
| author_facet | Da-ae Gwon Joo Yeon Seok Gyoo Yeol Jung Jeong Wook Lee |
| author_sort | Da-ae Gwon |
| collection | DOAJ |
| description | Violacein is a naturally occurring purple pigment, widely used in cosmetics and has potent antibacterial and antiviral properties. Violacein can be produced from tryptophan, consequently sufficient tryptophan biosynthesis is the key to violacein production. However, the complicated biosynthetic pathways and regulatory mechanisms often make the tryptophan overproduction challenging in <i>Escherichia coli</i>. In this study, we used the adaptive laboratory evolution (ALE) strategy to improve violacein production using galactose as a carbon source. During the ALE, a tryptophan-responsive biosensor was employed to provide selection pressure to enrich tryptophan-producing cells. From the biosensor-assisted ALE, we obtained an evolved population of cells capable of effectively catabolizing galactose to tryptophan and subsequently used the population to obtain the best violacein producer. In addition, whole-genome sequencing of the evolved strain identified point mutations beneficial to the overproduction. Overall, we demonstrated that the biosensor-assisted ALE strategy could be used to rapidly and selectively evolve the producers to yield high violacein production. |
| first_indexed | 2024-03-10T10:14:23Z |
| format | Article |
| id | doaj.art-20dc4883dc5b475981f371436d2c3cd1 |
| institution | Directory Open Access Journal |
| issn | 1661-6596 1422-0067 |
| language | English |
| last_indexed | 2024-03-10T10:14:23Z |
| publishDate | 2021-06-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | International Journal of Molecular Sciences |
| spelling | doaj.art-20dc4883dc5b475981f371436d2c3cd12023-11-22T00:53:27ZengMDPI AGInternational Journal of Molecular Sciences1661-65961422-00672021-06-012212659410.3390/ijms22126594Biosensor-Assisted Adaptive Laboratory Evolution for Violacein ProductionDa-ae Gwon0Joo Yeon Seok1Gyoo Yeol Jung2Jeong Wook Lee3Department of Chemical Engineering, Pohang University of Science and Technology, 77 Cheongam-ro, Nam-gu, Pohang, Gyeongbuk 37673, KoreaSchool of Interdisciplinary Bioscience and Bioengineering, Pohang University of Science, 77 Cheongam-ro, Nam-gu, Pohang, Gyeongbuk 37673, KoreaDepartment of Chemical Engineering, Pohang University of Science and Technology, 77 Cheongam-ro, Nam-gu, Pohang, Gyeongbuk 37673, KoreaDepartment of Chemical Engineering, Pohang University of Science and Technology, 77 Cheongam-ro, Nam-gu, Pohang, Gyeongbuk 37673, KoreaViolacein is a naturally occurring purple pigment, widely used in cosmetics and has potent antibacterial and antiviral properties. Violacein can be produced from tryptophan, consequently sufficient tryptophan biosynthesis is the key to violacein production. However, the complicated biosynthetic pathways and regulatory mechanisms often make the tryptophan overproduction challenging in <i>Escherichia coli</i>. In this study, we used the adaptive laboratory evolution (ALE) strategy to improve violacein production using galactose as a carbon source. During the ALE, a tryptophan-responsive biosensor was employed to provide selection pressure to enrich tryptophan-producing cells. From the biosensor-assisted ALE, we obtained an evolved population of cells capable of effectively catabolizing galactose to tryptophan and subsequently used the population to obtain the best violacein producer. In addition, whole-genome sequencing of the evolved strain identified point mutations beneficial to the overproduction. Overall, we demonstrated that the biosensor-assisted ALE strategy could be used to rapidly and selectively evolve the producers to yield high violacein production.https://www.mdpi.com/1422-0067/22/12/6594violaceintryptophanadaptive laboratory evolutionbiosensor |
| spellingShingle | Da-ae Gwon Joo Yeon Seok Gyoo Yeol Jung Jeong Wook Lee Biosensor-Assisted Adaptive Laboratory Evolution for Violacein Production International Journal of Molecular Sciences violacein tryptophan adaptive laboratory evolution biosensor |
| title | Biosensor-Assisted Adaptive Laboratory Evolution for Violacein Production |
| title_full | Biosensor-Assisted Adaptive Laboratory Evolution for Violacein Production |
| title_fullStr | Biosensor-Assisted Adaptive Laboratory Evolution for Violacein Production |
| title_full_unstemmed | Biosensor-Assisted Adaptive Laboratory Evolution for Violacein Production |
| title_short | Biosensor-Assisted Adaptive Laboratory Evolution for Violacein Production |
| title_sort | biosensor assisted adaptive laboratory evolution for violacein production |
| topic | violacein tryptophan adaptive laboratory evolution biosensor |
| url | https://www.mdpi.com/1422-0067/22/12/6594 |
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