Evaluation of Plasma Circulating Cell Free DNA Concentration and Integrity in Patients with Prostate Cancer in Jamaica: A Preliminary Study
Background: Cell free circulating DNA (cfcDNA) is a promising diagnostic tool for prostate cancer (PCa). This study aimed to measure the cfcDNA concentration and integrity in PCa patients using quantitative polymerase chain reaction (qPCR) analysis. This study also assessed the correlation between t...
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| Format: | Article |
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MDPI AG
2020-09-01
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| Series: | Diseases |
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| Online Access: | https://www.mdpi.com/2079-9721/8/3/34 |
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| author | Andrew Condappa Donovan McGrowder William Aiken Wayne McLaughlin Maxine Gossell-Williams |
| author_facet | Andrew Condappa Donovan McGrowder William Aiken Wayne McLaughlin Maxine Gossell-Williams |
| author_sort | Andrew Condappa |
| collection | DOAJ |
| description | Background: Cell free circulating DNA (cfcDNA) is a promising diagnostic tool for prostate cancer (PCa). This study aimed to measure the cfcDNA concentration and integrity in PCa patients using quantitative polymerase chain reaction (qPCR) analysis. This study also assessed the correlation between these molecular biomarkers with total prostate-specific antigen (PSA), Gleason score, prostate volume, and age. Methods: Eleven PCa patients and 9 persons with benign prostatic hyperplasia (BPH) were recruited. Blood samples were collected before prostate biopsy and plasma quantified by qPCR amplification of the ALU 115 DNA sequence, with the ratio of ALU 247 to ALU 115 reflecting cfcDNA integrity. Results: There were no significant differences in median, interquartile range (IQR) cfcDNA concentration or cfcDNA integrity between the patients with PCa (47.9 (214.93) ng/mL; 0.61 (0.49)) and persons with BPH (41.5 (55.13) ng/mL, <i>p</i> = 0.382; 0.67 (0.45), <i>p</i> = 0.342). A weakly positive correlation exists between cfcDNA concentration and total PSA (r = 0.200, <i>p</i> = 0.555) but not with age or Gleason score in PCa patients. Conclusion: cfcDNA concentration was relatively nonsignificantly higher in PCa patients in comparison to persons with BPH, whereas cfcDNA integrity was similar in both groups. Though limited in sample size, this study shows that cfcDNA concentration may be a potentially valuable noninvasive biomarker for the diagnosis of PCa. |
| first_indexed | 2024-03-10T16:30:26Z |
| format | Article |
| id | doaj.art-20e3a5ae32484332aed8a76599300957 |
| institution | Directory Open Access Journal |
| issn | 2079-9721 |
| language | English |
| last_indexed | 2024-03-10T16:30:26Z |
| publishDate | 2020-09-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Diseases |
| spelling | doaj.art-20e3a5ae32484332aed8a765993009572023-11-20T12:49:57ZengMDPI AGDiseases2079-97212020-09-01833410.3390/diseases8030034Evaluation of Plasma Circulating Cell Free DNA Concentration and Integrity in Patients with Prostate Cancer in Jamaica: A Preliminary StudyAndrew Condappa0Donovan McGrowder1William Aiken2Wayne McLaughlin3Maxine Gossell-Williams4Department of Basic Medical Sciences (Pharmacology Section), Faculty of Medical Sciences, The University of the West Indies, Kingston 7, JamaicaDepartment of Pathology, Faculty of Medical Sciences, The University of the West Indies, Kingston 7, JamaicaDepartment of Surgery, Radiology, Anaesthesia and Intensive Care, Section of Surgery, Urology Division, Faculty of Medical Sciences, The University of the West Indies, Kingston 7, JamaicaDepartment of Basic Medical Sciences (Molecular Biology Section), Faculty of Medical Sciences, The University of the West Indies, Kingston 7, JamaicaDepartment of Basic Medical Sciences (Pharmacology Section), Faculty of Medical Sciences, The University of the West Indies, Kingston 7, JamaicaBackground: Cell free circulating DNA (cfcDNA) is a promising diagnostic tool for prostate cancer (PCa). This study aimed to measure the cfcDNA concentration and integrity in PCa patients using quantitative polymerase chain reaction (qPCR) analysis. This study also assessed the correlation between these molecular biomarkers with total prostate-specific antigen (PSA), Gleason score, prostate volume, and age. Methods: Eleven PCa patients and 9 persons with benign prostatic hyperplasia (BPH) were recruited. Blood samples were collected before prostate biopsy and plasma quantified by qPCR amplification of the ALU 115 DNA sequence, with the ratio of ALU 247 to ALU 115 reflecting cfcDNA integrity. Results: There were no significant differences in median, interquartile range (IQR) cfcDNA concentration or cfcDNA integrity between the patients with PCa (47.9 (214.93) ng/mL; 0.61 (0.49)) and persons with BPH (41.5 (55.13) ng/mL, <i>p</i> = 0.382; 0.67 (0.45), <i>p</i> = 0.342). A weakly positive correlation exists between cfcDNA concentration and total PSA (r = 0.200, <i>p</i> = 0.555) but not with age or Gleason score in PCa patients. Conclusion: cfcDNA concentration was relatively nonsignificantly higher in PCa patients in comparison to persons with BPH, whereas cfcDNA integrity was similar in both groups. Though limited in sample size, this study shows that cfcDNA concentration may be a potentially valuable noninvasive biomarker for the diagnosis of PCa.https://www.mdpi.com/2079-9721/8/3/34cell freecirculating DNAprostatecancerintegrity |
| spellingShingle | Andrew Condappa Donovan McGrowder William Aiken Wayne McLaughlin Maxine Gossell-Williams Evaluation of Plasma Circulating Cell Free DNA Concentration and Integrity in Patients with Prostate Cancer in Jamaica: A Preliminary Study Diseases cell free circulating DNA prostate cancer integrity |
| title | Evaluation of Plasma Circulating Cell Free DNA Concentration and Integrity in Patients with Prostate Cancer in Jamaica: A Preliminary Study |
| title_full | Evaluation of Plasma Circulating Cell Free DNA Concentration and Integrity in Patients with Prostate Cancer in Jamaica: A Preliminary Study |
| title_fullStr | Evaluation of Plasma Circulating Cell Free DNA Concentration and Integrity in Patients with Prostate Cancer in Jamaica: A Preliminary Study |
| title_full_unstemmed | Evaluation of Plasma Circulating Cell Free DNA Concentration and Integrity in Patients with Prostate Cancer in Jamaica: A Preliminary Study |
| title_short | Evaluation of Plasma Circulating Cell Free DNA Concentration and Integrity in Patients with Prostate Cancer in Jamaica: A Preliminary Study |
| title_sort | evaluation of plasma circulating cell free dna concentration and integrity in patients with prostate cancer in jamaica a preliminary study |
| topic | cell free circulating DNA prostate cancer integrity |
| url | https://www.mdpi.com/2079-9721/8/3/34 |
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