Evaluation of genetic parameters of 23 autosomal STR loci in a Southern Chinese Han population
Aim: To evaluate the 23 autosomal short tandem repeat (STR) loci included in GoldenEye™ 25 A kit using forensic human identification and paternity testing. Subjects and methods: In total, 3751 unrelated individuals from the Southern Chinese Han population were genotyped with the 5-dye GoldenEye™ 25 ...
| Main Authors: | , , , , , , |
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| Format: | Article |
| Language: | English |
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Taylor & Francis Group
2018-05-01
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| Series: | Annals of Human Biology |
| Subjects: | |
| Online Access: | http://dx.doi.org/10.1080/03014460.2018.1480731 |
| _version_ | 1797685553469063168 |
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| author | Hai-xia Li Dan Peng Ying Wang Ri-ga Wu Yin-ming Zhang Ran Li Hong-yu Sun |
| author_facet | Hai-xia Li Dan Peng Ying Wang Ri-ga Wu Yin-ming Zhang Ran Li Hong-yu Sun |
| author_sort | Hai-xia Li |
| collection | DOAJ |
| description | Aim: To evaluate the 23 autosomal short tandem repeat (STR) loci included in GoldenEye™ 25 A kit using forensic human identification and paternity testing. Subjects and methods: In total, 3751 unrelated individuals from the Southern Chinese Han population were genotyped with the 5-dye GoldenEye™ 25 A multiplex amplification system. PCR products were separated using arrayed capillary electrophoresis. Allele frequencies and forensic parameters for the 23 autosomal STR loci were statistically analysed. Results: A total of 344 alleles were observed, with corresponding allelic frequencies ranging from 0.0001–0.5519 for the 23 STR loci. No significant deviation from the Hardy-Weinberg equilibrium and linkage disequilibrium was observed. The combined power of discrimination (CPD) was 1–1.6290 × 10−28 and the combined power of exclusion (CPE) was 0.999 999 999 89 and 0.999 999 286 93 for trio and duo cases, respectively. From 3865 meioses, 87 mutation events were discovered. The mutation rate varied from 0–0.00285 for each locus. One-step mutation accounted for 94.25% of total mutations. The ratio of paternal vs maternal mutation was 3.76:1.13 kinds of n/(n + 1) heterozygote genotypes were observed. Conclusions: The results show that 23 STR loci of GoldenEye™ 25 A kit are highly polymorphic in the Southern Chinese Han population, indicating the kit is suitable for forensic application. |
| first_indexed | 2024-03-12T00:46:48Z |
| format | Article |
| id | doaj.art-20eba12e9bd34f618593816c580077a1 |
| institution | Directory Open Access Journal |
| issn | 0301-4460 1464-5033 |
| language | English |
| last_indexed | 2024-03-12T00:46:48Z |
| publishDate | 2018-05-01 |
| publisher | Taylor & Francis Group |
| record_format | Article |
| series | Annals of Human Biology |
| spelling | doaj.art-20eba12e9bd34f618593816c580077a12023-09-14T15:36:10ZengTaylor & Francis GroupAnnals of Human Biology0301-44601464-50332018-05-0145435936410.1080/03014460.2018.14807311480731Evaluation of genetic parameters of 23 autosomal STR loci in a Southern Chinese Han populationHai-xia Li0Dan Peng1Ying Wang2Ri-ga Wu3Yin-ming Zhang4Ran Li5Hong-yu Sun6Sun Yat-sen UniversitySun Yat-sen UniversitySun Yat-sen UniversitySun Yat-sen UniversitySun Yat-sen UniversitySun Yat-sen UniversitySun Yat-sen UniversityAim: To evaluate the 23 autosomal short tandem repeat (STR) loci included in GoldenEye™ 25 A kit using forensic human identification and paternity testing. Subjects and methods: In total, 3751 unrelated individuals from the Southern Chinese Han population were genotyped with the 5-dye GoldenEye™ 25 A multiplex amplification system. PCR products were separated using arrayed capillary electrophoresis. Allele frequencies and forensic parameters for the 23 autosomal STR loci were statistically analysed. Results: A total of 344 alleles were observed, with corresponding allelic frequencies ranging from 0.0001–0.5519 for the 23 STR loci. No significant deviation from the Hardy-Weinberg equilibrium and linkage disequilibrium was observed. The combined power of discrimination (CPD) was 1–1.6290 × 10−28 and the combined power of exclusion (CPE) was 0.999 999 999 89 and 0.999 999 286 93 for trio and duo cases, respectively. From 3865 meioses, 87 mutation events were discovered. The mutation rate varied from 0–0.00285 for each locus. One-step mutation accounted for 94.25% of total mutations. The ratio of paternal vs maternal mutation was 3.76:1.13 kinds of n/(n + 1) heterozygote genotypes were observed. Conclusions: The results show that 23 STR loci of GoldenEye™ 25 A kit are highly polymorphic in the Southern Chinese Han population, indicating the kit is suitable for forensic application.http://dx.doi.org/10.1080/03014460.2018.1480731strgoldeneyetm 25asouthern chinese han populationmutation |
| spellingShingle | Hai-xia Li Dan Peng Ying Wang Ri-ga Wu Yin-ming Zhang Ran Li Hong-yu Sun Evaluation of genetic parameters of 23 autosomal STR loci in a Southern Chinese Han population Annals of Human Biology str goldeneyetm 25a southern chinese han population mutation |
| title | Evaluation of genetic parameters of 23 autosomal STR loci in a Southern Chinese Han population |
| title_full | Evaluation of genetic parameters of 23 autosomal STR loci in a Southern Chinese Han population |
| title_fullStr | Evaluation of genetic parameters of 23 autosomal STR loci in a Southern Chinese Han population |
| title_full_unstemmed | Evaluation of genetic parameters of 23 autosomal STR loci in a Southern Chinese Han population |
| title_short | Evaluation of genetic parameters of 23 autosomal STR loci in a Southern Chinese Han population |
| title_sort | evaluation of genetic parameters of 23 autosomal str loci in a southern chinese han population |
| topic | str goldeneyetm 25a southern chinese han population mutation |
| url | http://dx.doi.org/10.1080/03014460.2018.1480731 |
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