Antimicrobial Photodynamic Inactivation: An Alternative for Group B <i>Streptococcus</i> Vaginal Colonization in a Murine Experimental Model
Background: <i>Streptococcus agalactiae</i>, referred to as Group B <i>Streptococcus</i> (GBS), is a prominent bacterium causing life-threatening neonatal infections. Although antibiotics are efficient against GBS, growing antibiotic resistance forces the search for alternati...
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MDPI AG
2023-04-01
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| Series: | Antioxidants |
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| Online Access: | https://www.mdpi.com/2076-3921/12/4/847 |
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| author | Michał K. Pierański Jan G. Kosiński Klaudia Szymczak Piotr Sadowski Mariusz Grinholc |
| author_facet | Michał K. Pierański Jan G. Kosiński Klaudia Szymczak Piotr Sadowski Mariusz Grinholc |
| author_sort | Michał K. Pierański |
| collection | DOAJ |
| description | Background: <i>Streptococcus agalactiae</i>, referred to as Group B <i>Streptococcus</i> (GBS), is a prominent bacterium causing life-threatening neonatal infections. Although antibiotics are efficient against GBS, growing antibiotic resistance forces the search for alternative treatments and/or prevention approaches. Antimicrobial photodynamic inactivation (aPDI) appears to be a potent alternative non-antibiotic strategy against GBS. Methods: The effect of rose bengal aPDI on various GBS serotypes, <i>Lactobacillus</i> species, human eukaryotic cell lines and microbial vaginal flora composition was evaluated. Results: RB-mediated aPDI was evidenced to exert high bactericidal efficacy towards <i>S. agalactiae</i> in vitro (>4 log<sub>10</sub> units of viability reduction for planktonic and >2 log<sub>10</sub> units for multispecies biofilm culture) and in vivo (ca. 2 log<sub>10</sub> units of viability reduction in mice vaginal GBS colonization model) in microbiological and metagenomic analyses. At the same time, RB-mediated aPDI was evidenced to be not mutagenic and safe for human vaginal cells, as well as capable of maintaining the balance and viability of vaginal microbial flora. Conclusions: aPDI can efficiently kill GBS and serve as an alternative approach against GBS vaginal colonization and/or infections. |
| first_indexed | 2024-03-11T05:17:39Z |
| format | Article |
| id | doaj.art-20ed9e1523cf4911a92e3e46629e47b6 |
| institution | Directory Open Access Journal |
| issn | 2076-3921 |
| language | English |
| last_indexed | 2024-03-11T05:17:39Z |
| publishDate | 2023-04-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Antioxidants |
| spelling | doaj.art-20ed9e1523cf4911a92e3e46629e47b62023-11-17T18:05:09ZengMDPI AGAntioxidants2076-39212023-04-0112484710.3390/antiox12040847Antimicrobial Photodynamic Inactivation: An Alternative for Group B <i>Streptococcus</i> Vaginal Colonization in a Murine Experimental ModelMichał K. Pierański0Jan G. Kosiński1Klaudia Szymczak2Piotr Sadowski3Mariusz Grinholc4Laboratory of Photobiology and Molecular Diagnostics, Intercollegiate Faculty of Biotechnology University of Gdańsk and Medical University of Gdańsk, University of Gdańsk, 80-307 Gdańsk, PolandDepartment of Computational Biology, Institute of Molecular Biology and Biotechnology, Faculty of Biology, Adam Mickiewicz University in Poznań, 61-712 Poznań, PolandLaboratory of Photobiology and Molecular Diagnostics, Intercollegiate Faculty of Biotechnology University of Gdańsk and Medical University of Gdańsk, University of Gdańsk, 80-307 Gdańsk, PolandDepartment of Pathomorphology, University Hospital in Kraków, 31-501 Kraków, PolandLaboratory of Photobiology and Molecular Diagnostics, Intercollegiate Faculty of Biotechnology University of Gdańsk and Medical University of Gdańsk, University of Gdańsk, 80-307 Gdańsk, PolandBackground: <i>Streptococcus agalactiae</i>, referred to as Group B <i>Streptococcus</i> (GBS), is a prominent bacterium causing life-threatening neonatal infections. Although antibiotics are efficient against GBS, growing antibiotic resistance forces the search for alternative treatments and/or prevention approaches. Antimicrobial photodynamic inactivation (aPDI) appears to be a potent alternative non-antibiotic strategy against GBS. Methods: The effect of rose bengal aPDI on various GBS serotypes, <i>Lactobacillus</i> species, human eukaryotic cell lines and microbial vaginal flora composition was evaluated. Results: RB-mediated aPDI was evidenced to exert high bactericidal efficacy towards <i>S. agalactiae</i> in vitro (>4 log<sub>10</sub> units of viability reduction for planktonic and >2 log<sub>10</sub> units for multispecies biofilm culture) and in vivo (ca. 2 log<sub>10</sub> units of viability reduction in mice vaginal GBS colonization model) in microbiological and metagenomic analyses. At the same time, RB-mediated aPDI was evidenced to be not mutagenic and safe for human vaginal cells, as well as capable of maintaining the balance and viability of vaginal microbial flora. Conclusions: aPDI can efficiently kill GBS and serve as an alternative approach against GBS vaginal colonization and/or infections.https://www.mdpi.com/2076-3921/12/4/847<i>Streptococcus agalactiae</i>biofilmmurine modelphotoinactivationrose bengalvaginal microbiome |
| spellingShingle | Michał K. Pierański Jan G. Kosiński Klaudia Szymczak Piotr Sadowski Mariusz Grinholc Antimicrobial Photodynamic Inactivation: An Alternative for Group B <i>Streptococcus</i> Vaginal Colonization in a Murine Experimental Model Antioxidants <i>Streptococcus agalactiae</i> biofilm murine model photoinactivation rose bengal vaginal microbiome |
| title | Antimicrobial Photodynamic Inactivation: An Alternative for Group B <i>Streptococcus</i> Vaginal Colonization in a Murine Experimental Model |
| title_full | Antimicrobial Photodynamic Inactivation: An Alternative for Group B <i>Streptococcus</i> Vaginal Colonization in a Murine Experimental Model |
| title_fullStr | Antimicrobial Photodynamic Inactivation: An Alternative for Group B <i>Streptococcus</i> Vaginal Colonization in a Murine Experimental Model |
| title_full_unstemmed | Antimicrobial Photodynamic Inactivation: An Alternative for Group B <i>Streptococcus</i> Vaginal Colonization in a Murine Experimental Model |
| title_short | Antimicrobial Photodynamic Inactivation: An Alternative for Group B <i>Streptococcus</i> Vaginal Colonization in a Murine Experimental Model |
| title_sort | antimicrobial photodynamic inactivation an alternative for group b i streptococcus i vaginal colonization in a murine experimental model |
| topic | <i>Streptococcus agalactiae</i> biofilm murine model photoinactivation rose bengal vaginal microbiome |
| url | https://www.mdpi.com/2076-3921/12/4/847 |
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