The mechanism of Naringin-enhanced remyelination after spinal cord injury
Our previous study revealed that intragastric administration of naringin improved remyelination in rats with spinal cord injury and promoted the recovery of neurological function of the injured spinal cord. This study sought to reveal the mechanisms by which naringin improves oligodendrocyte precurs...
| Main Authors: | , , , , , , |
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| Format: | Article |
| Language: | English |
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Wolters Kluwer Medknow Publications
2017-01-01
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| Series: | Neural Regeneration Research |
| Subjects: | |
| Online Access: | http://www.nrronline.org/article.asp?issn=1673-5374;year=2017;volume=12;issue=3;spage=470;epage=477;aulast=Rong |
| _version_ | 1811298330919043072 |
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| author | Wei Rong Yong-wei Pan Xu Cai Fei Song Zhe Zhao Song-hua Xiao Cheng Zhang |
| author_facet | Wei Rong Yong-wei Pan Xu Cai Fei Song Zhe Zhao Song-hua Xiao Cheng Zhang |
| author_sort | Wei Rong |
| collection | DOAJ |
| description | Our previous study revealed that intragastric administration of naringin improved remyelination in rats with spinal cord injury and promoted the recovery of neurological function of the injured spinal cord. This study sought to reveal the mechanisms by which naringin improves oligodendrocyte precursor cell differentiation and maturation, and promotes remyelination. Spinal cord injury was induced in rats by the weight-drop method. Naringin was intragastrically administered daily (20, 40 mg/kg) for 4 weeks after spinal cord injury induction. Behavioral assessment, histopathological staining, immunofluorescence spectroscopy, ultrastructural analysis and biochemical assays were employed. Naringin treatment remarkably mitigated demyelination in the white matter, increased the quality of myelinated nerve fibers and myelin sheath thickness, promoted oligodendrocyte precursor cell differentiation by upregulating the expression of NKx2.2 and 2′3′-cyclic nucleotide 3′-phosphodiesterase, and inhibited β-catenin expression and glycogen synthase kinase-3β (GSK-3β) phosphorylation. These findings indicate that naringin treatment regulates oligodendrocyte precursor cell differentiation and promotes remyelination after spinal cord injury through the β-catenin/GSK-3β signaling pathway. |
| first_indexed | 2024-04-13T06:17:53Z |
| format | Article |
| id | doaj.art-20ede547fad442faacbdd4689e1f5a56 |
| institution | Directory Open Access Journal |
| issn | 1673-5374 |
| language | English |
| last_indexed | 2024-04-13T06:17:53Z |
| publishDate | 2017-01-01 |
| publisher | Wolters Kluwer Medknow Publications |
| record_format | Article |
| series | Neural Regeneration Research |
| spelling | doaj.art-20ede547fad442faacbdd4689e1f5a562022-12-22T02:58:45ZengWolters Kluwer Medknow PublicationsNeural Regeneration Research1673-53742017-01-0112347047710.4103/1673-5374.202923The mechanism of Naringin-enhanced remyelination after spinal cord injuryWei RongYong-wei PanXu CaiFei SongZhe ZhaoSong-hua XiaoCheng ZhangOur previous study revealed that intragastric administration of naringin improved remyelination in rats with spinal cord injury and promoted the recovery of neurological function of the injured spinal cord. This study sought to reveal the mechanisms by which naringin improves oligodendrocyte precursor cell differentiation and maturation, and promotes remyelination. Spinal cord injury was induced in rats by the weight-drop method. Naringin was intragastrically administered daily (20, 40 mg/kg) for 4 weeks after spinal cord injury induction. Behavioral assessment, histopathological staining, immunofluorescence spectroscopy, ultrastructural analysis and biochemical assays were employed. Naringin treatment remarkably mitigated demyelination in the white matter, increased the quality of myelinated nerve fibers and myelin sheath thickness, promoted oligodendrocyte precursor cell differentiation by upregulating the expression of NKx2.2 and 2′3′-cyclic nucleotide 3′-phosphodiesterase, and inhibited β-catenin expression and glycogen synthase kinase-3β (GSK-3β) phosphorylation. These findings indicate that naringin treatment regulates oligodendrocyte precursor cell differentiation and promotes remyelination after spinal cord injury through the β-catenin/GSK-3β signaling pathway.http://www.nrronline.org/article.asp?issn=1673-5374;year=2017;volume=12;issue=3;spage=470;epage=477;aulast=Rongnerve regeneration; spinal cord injury; naringin; remyelination; oligodendrocyte precursor cells; oligodendrocytes; β-catenin; glycogen synthase kinase-3β; NKx2.2; 2'3'-cyclic nucleotide 3'phodiesterase; behavioral assessment; neural regeneration |
| spellingShingle | Wei Rong Yong-wei Pan Xu Cai Fei Song Zhe Zhao Song-hua Xiao Cheng Zhang The mechanism of Naringin-enhanced remyelination after spinal cord injury Neural Regeneration Research nerve regeneration; spinal cord injury; naringin; remyelination; oligodendrocyte precursor cells; oligodendrocytes; β-catenin; glycogen synthase kinase-3β; NKx2.2; 2' 3'-cyclic nucleotide 3'phodiesterase; behavioral assessment; neural regeneration |
| title | The mechanism of Naringin-enhanced remyelination after spinal cord injury |
| title_full | The mechanism of Naringin-enhanced remyelination after spinal cord injury |
| title_fullStr | The mechanism of Naringin-enhanced remyelination after spinal cord injury |
| title_full_unstemmed | The mechanism of Naringin-enhanced remyelination after spinal cord injury |
| title_short | The mechanism of Naringin-enhanced remyelination after spinal cord injury |
| title_sort | mechanism of naringin enhanced remyelination after spinal cord injury |
| topic | nerve regeneration; spinal cord injury; naringin; remyelination; oligodendrocyte precursor cells; oligodendrocytes; β-catenin; glycogen synthase kinase-3β; NKx2.2; 2' 3'-cyclic nucleotide 3'phodiesterase; behavioral assessment; neural regeneration |
| url | http://www.nrronline.org/article.asp?issn=1673-5374;year=2017;volume=12;issue=3;spage=470;epage=477;aulast=Rong |
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