Effects of Host Plants on Bacterial Community Structure in Larvae Midgut of <i>Spodoptera frugiperda</i>
The fall armyworm (FAW), <i>Spodoptera frugiperda</i><i>,</i> is one of the most important invasive species and causes great damage to various host crops in China. In this study, the diversity and function of gut bacteria in the 5th instar larvae of FAW fed on maize, wheat, p...
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MDPI AG
2022-04-01
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| Series: | Insects |
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| Online Access: | https://www.mdpi.com/2075-4450/13/4/373 |
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| author | Ya-Ping Chen Ya-Hong Li Zhong-Xiang Sun E-Wei Du Zhi-Hui Lu Hao Li Fu-Rong Gui |
| author_facet | Ya-Ping Chen Ya-Hong Li Zhong-Xiang Sun E-Wei Du Zhi-Hui Lu Hao Li Fu-Rong Gui |
| author_sort | Ya-Ping Chen |
| collection | DOAJ |
| description | The fall armyworm (FAW), <i>Spodoptera frugiperda</i><i>,</i> is one of the most important invasive species and causes great damage to various host crops in China. In this study, the diversity and function of gut bacteria in the 5th instar larvae of FAW fed on maize, wheat, potato and tobacco leaves were analyzed through 16S rRNA sequencing. A total of 1324.25 ± 199.73, 1313.5 ± 74.87, 1873.00 ± 190.66 and 1435.25 ± 139.87 operational taxonomic units (OTUs) from the gut of FAW fed on these four different host plants were detected, respectively. Firmicutes, Proteobacteria and Bacteroidetes were the most abundant bacterial phyla. Beta diversity analysis showed that the gut bacterial community structure of larvae fed on different host plants was significantly differentiated. At the genus level, the abundance of <i>Enterococcus</i> in larvae fed on wheat was significantly lower than those fed on the other three host plants. <i>Enterobacter</i> and <i>ZOR0006</i> were dominant in FAW fed on tobacco leaves, and in low abundance in larvae fed on wheat. Interestingly, when fed on Solanaceae (tobacco and potato) leaves which contained relative higher levels of toxic secondary metabolites than Gramineae (wheat and maize), the genera <i>Enterococcus, Enterobacter</i> and <i>Acinetobacter</i> were significantly enriched. The results indicated that gut bacteria were related to the detoxification and adaptation of toxic secondary metabolites of host plants in FAW. Further analysis showed that replication, repair and nucleotide metabolism functions were enriched in the gut bacteria of larvae fed on tobacco and potato. In conclusion, the gut bacterial diversity and community composition in FAW larvae fed on different host plants showed significant differences, and the insect is likely to regulate their gut bacteria for adaptation to different host plants. |
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| language | English |
| last_indexed | 2024-03-09T10:32:45Z |
| publishDate | 2022-04-01 |
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| series | Insects |
| spelling | doaj.art-20f165d000f546c6a3ce6f056fe73aca2023-12-01T21:05:58ZengMDPI AGInsects2075-44502022-04-0113437310.3390/insects13040373Effects of Host Plants on Bacterial Community Structure in Larvae Midgut of <i>Spodoptera frugiperda</i>Ya-Ping Chen0Ya-Hong Li1Zhong-Xiang Sun2E-Wei Du3Zhi-Hui Lu4Hao Li5Fu-Rong Gui6State Key Laboratory for Conservation and Utilization of Bioresources in Yunnan, Yunnan Agricultural University, Kunming 650201, ChinaYunnan Plant Protection and Quarantine Station, Kunming 650034, ChinaState Key Laboratory for Conservation and Utilization of Bioresources in Yunnan, Yunnan Agricultural University, Kunming 650201, ChinaState Key Laboratory for Conservation and Utilization of Bioresources in Yunnan, Yunnan Agricultural University, Kunming 650201, ChinaState Key Laboratory for Conservation and Utilization of Bioresources in Yunnan, Yunnan Agricultural University, Kunming 650201, ChinaState Key Laboratory for Conservation and Utilization of Bioresources in Yunnan, Yunnan Agricultural University, Kunming 650201, ChinaState Key Laboratory for Conservation and Utilization of Bioresources in Yunnan, Yunnan Agricultural University, Kunming 650201, ChinaThe fall armyworm (FAW), <i>Spodoptera frugiperda</i><i>,</i> is one of the most important invasive species and causes great damage to various host crops in China. In this study, the diversity and function of gut bacteria in the 5th instar larvae of FAW fed on maize, wheat, potato and tobacco leaves were analyzed through 16S rRNA sequencing. A total of 1324.25 ± 199.73, 1313.5 ± 74.87, 1873.00 ± 190.66 and 1435.25 ± 139.87 operational taxonomic units (OTUs) from the gut of FAW fed on these four different host plants were detected, respectively. Firmicutes, Proteobacteria and Bacteroidetes were the most abundant bacterial phyla. Beta diversity analysis showed that the gut bacterial community structure of larvae fed on different host plants was significantly differentiated. At the genus level, the abundance of <i>Enterococcus</i> in larvae fed on wheat was significantly lower than those fed on the other three host plants. <i>Enterobacter</i> and <i>ZOR0006</i> were dominant in FAW fed on tobacco leaves, and in low abundance in larvae fed on wheat. Interestingly, when fed on Solanaceae (tobacco and potato) leaves which contained relative higher levels of toxic secondary metabolites than Gramineae (wheat and maize), the genera <i>Enterococcus, Enterobacter</i> and <i>Acinetobacter</i> were significantly enriched. The results indicated that gut bacteria were related to the detoxification and adaptation of toxic secondary metabolites of host plants in FAW. Further analysis showed that replication, repair and nucleotide metabolism functions were enriched in the gut bacteria of larvae fed on tobacco and potato. In conclusion, the gut bacterial diversity and community composition in FAW larvae fed on different host plants showed significant differences, and the insect is likely to regulate their gut bacteria for adaptation to different host plants.https://www.mdpi.com/2075-4450/13/4/373<i>Spodoptera frugiperda</i>invasive insectgut bacteriahost plant16S rRNAtoxic secondary metabolites |
| spellingShingle | Ya-Ping Chen Ya-Hong Li Zhong-Xiang Sun E-Wei Du Zhi-Hui Lu Hao Li Fu-Rong Gui Effects of Host Plants on Bacterial Community Structure in Larvae Midgut of <i>Spodoptera frugiperda</i> Insects <i>Spodoptera frugiperda</i> invasive insect gut bacteria host plant 16S rRNA toxic secondary metabolites |
| title | Effects of Host Plants on Bacterial Community Structure in Larvae Midgut of <i>Spodoptera frugiperda</i> |
| title_full | Effects of Host Plants on Bacterial Community Structure in Larvae Midgut of <i>Spodoptera frugiperda</i> |
| title_fullStr | Effects of Host Plants on Bacterial Community Structure in Larvae Midgut of <i>Spodoptera frugiperda</i> |
| title_full_unstemmed | Effects of Host Plants on Bacterial Community Structure in Larvae Midgut of <i>Spodoptera frugiperda</i> |
| title_short | Effects of Host Plants on Bacterial Community Structure in Larvae Midgut of <i>Spodoptera frugiperda</i> |
| title_sort | effects of host plants on bacterial community structure in larvae midgut of i spodoptera frugiperda i |
| topic | <i>Spodoptera frugiperda</i> invasive insect gut bacteria host plant 16S rRNA toxic secondary metabolites |
| url | https://www.mdpi.com/2075-4450/13/4/373 |
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