Minimal impacts on the wheat microbiome when Trichoderma gamsii T6085 is applied as a biocontrol agent to manage fusarium head blight disease
Fusarium head blight (FHB) is a major fungal disease that causes severe yield and quality loss in wheat. Biological control can be integrated with other management strategies to control FHB. For this purpose, Trichoderma gamsii strain T6085 is a potential biocontrol agent to limit the infection of F...
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2022-09-01
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| Series: | Frontiers in Microbiology |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fmicb.2022.972016/full |
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| author | Arshani S. Alukumbura Alessandro Bigi Sabrina Sarrocco W. G. Dilantha Fernando Giovanni Vannacci Marco Mazzoncini Marco Mazzoncini Matthew G. Bakker |
| author_facet | Arshani S. Alukumbura Alessandro Bigi Sabrina Sarrocco W. G. Dilantha Fernando Giovanni Vannacci Marco Mazzoncini Marco Mazzoncini Matthew G. Bakker |
| author_sort | Arshani S. Alukumbura |
| collection | DOAJ |
| description | Fusarium head blight (FHB) is a major fungal disease that causes severe yield and quality loss in wheat. Biological control can be integrated with other management strategies to control FHB. For this purpose, Trichoderma gamsii strain T6085 is a potential biocontrol agent to limit the infection of F. graminearum and F. culmorum in wheat. However, the possible impacts of T. gamsii T6085 on the broader microbiome associated with the wheat plant are not currently understood. Therefore, we identified bacteria and fungi associated with different wheat tissues, including assessment of their relative abundances and dynamics in response to the application of T6085 and over time, using amplicon sequencing. Residues of the prior year’s wheat crop and the current year’s wheat spikes were collected at multiple time points, and kernel samples were collected at harvest. DNA was extracted from the collected wheat tissues, and amplicon sequencing was performed to profile microbiomes using 16S v4 rRNA amplicons for bacteria and ITS2 amplicons for fungi. Quantitative PCR was performed to evaluate the absolute abundances of F. graminearum and T. gamsii in different wheat tissues. Disease progression was tracked visually during the growing season, revealing that FHB severity and incidence were significantly reduced when T6085 was applied to wheat spikes at anthesis. However, treatment with T6085 did not lessen the F. graminearum abundance in wheat spikes or kernels. There were substantial changes in F. graminearum abundance over time; in crop residue, pathogen abundance was highest at the initial time point and declined over time, while in wheat spikes, pathogen abundance increased significantly over time. The predominant bacterial taxa in wheat spikes and kernels were Pseudomonas, Enterobacter, and Pantoea, while Alternaria and Fusarium were the dominant fungal groups. Although the microbiome structure changed substantially over time, there were no community-scale rearrangements due to the T6085 treatment. The work suggests several other taxa that could be explored as potential biocontrol agents to integrate with T6085 treatment. However, the timing and the type of T6085 application need to be improved to give more advantages for T6085 to colonize and reduce the F. graminearum inoculum in the field. |
| first_indexed | 2024-04-12T03:12:51Z |
| format | Article |
| id | doaj.art-20f18b9b464545c2affb9117d87ff431 |
| institution | Directory Open Access Journal |
| issn | 1664-302X |
| language | English |
| last_indexed | 2024-04-12T03:12:51Z |
| publishDate | 2022-09-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Microbiology |
| spelling | doaj.art-20f18b9b464545c2affb9117d87ff4312022-12-22T03:50:17ZengFrontiers Media S.A.Frontiers in Microbiology1664-302X2022-09-011310.3389/fmicb.2022.972016972016Minimal impacts on the wheat microbiome when Trichoderma gamsii T6085 is applied as a biocontrol agent to manage fusarium head blight diseaseArshani S. Alukumbura0Alessandro Bigi1Sabrina Sarrocco2W. G. Dilantha Fernando3Giovanni Vannacci4Marco Mazzoncini5Marco Mazzoncini6Matthew G. Bakker7Department of Plant Science, University of Manitoba, Winnipeg, MB, CanadaDepartment of Agriculture, Food and Environment, University of Pisa, Pisa, ItalyDepartment of Agriculture, Food and Environment, University of Pisa, Pisa, ItalyDepartment of Plant Science, University of Manitoba, Winnipeg, MB, CanadaDepartment of Agriculture, Food and Environment, University of Pisa, Pisa, ItalyDepartment of Agriculture, Food and Environment, University of Pisa, Pisa, ItalyCenter of Agro-Environmental Research “Enrico Avanzi,” University of Pisa, Pisa, ItalyDepartment of Microbiology, University of Manitoba, Winnipeg, MB, CanadaFusarium head blight (FHB) is a major fungal disease that causes severe yield and quality loss in wheat. Biological control can be integrated with other management strategies to control FHB. For this purpose, Trichoderma gamsii strain T6085 is a potential biocontrol agent to limit the infection of F. graminearum and F. culmorum in wheat. However, the possible impacts of T. gamsii T6085 on the broader microbiome associated with the wheat plant are not currently understood. Therefore, we identified bacteria and fungi associated with different wheat tissues, including assessment of their relative abundances and dynamics in response to the application of T6085 and over time, using amplicon sequencing. Residues of the prior year’s wheat crop and the current year’s wheat spikes were collected at multiple time points, and kernel samples were collected at harvest. DNA was extracted from the collected wheat tissues, and amplicon sequencing was performed to profile microbiomes using 16S v4 rRNA amplicons for bacteria and ITS2 amplicons for fungi. Quantitative PCR was performed to evaluate the absolute abundances of F. graminearum and T. gamsii in different wheat tissues. Disease progression was tracked visually during the growing season, revealing that FHB severity and incidence were significantly reduced when T6085 was applied to wheat spikes at anthesis. However, treatment with T6085 did not lessen the F. graminearum abundance in wheat spikes or kernels. There were substantial changes in F. graminearum abundance over time; in crop residue, pathogen abundance was highest at the initial time point and declined over time, while in wheat spikes, pathogen abundance increased significantly over time. The predominant bacterial taxa in wheat spikes and kernels were Pseudomonas, Enterobacter, and Pantoea, while Alternaria and Fusarium were the dominant fungal groups. Although the microbiome structure changed substantially over time, there were no community-scale rearrangements due to the T6085 treatment. The work suggests several other taxa that could be explored as potential biocontrol agents to integrate with T6085 treatment. However, the timing and the type of T6085 application need to be improved to give more advantages for T6085 to colonize and reduce the F. graminearum inoculum in the field.https://www.frontiersin.org/articles/10.3389/fmicb.2022.972016/fullFusarium graminearumTriticum durum16S rRNAITSmicrobiomefusarium head blight |
| spellingShingle | Arshani S. Alukumbura Alessandro Bigi Sabrina Sarrocco W. G. Dilantha Fernando Giovanni Vannacci Marco Mazzoncini Marco Mazzoncini Matthew G. Bakker Minimal impacts on the wheat microbiome when Trichoderma gamsii T6085 is applied as a biocontrol agent to manage fusarium head blight disease Frontiers in Microbiology Fusarium graminearum Triticum durum 16S rRNA ITS microbiome fusarium head blight |
| title | Minimal impacts on the wheat microbiome when Trichoderma gamsii T6085 is applied as a biocontrol agent to manage fusarium head blight disease |
| title_full | Minimal impacts on the wheat microbiome when Trichoderma gamsii T6085 is applied as a biocontrol agent to manage fusarium head blight disease |
| title_fullStr | Minimal impacts on the wheat microbiome when Trichoderma gamsii T6085 is applied as a biocontrol agent to manage fusarium head blight disease |
| title_full_unstemmed | Minimal impacts on the wheat microbiome when Trichoderma gamsii T6085 is applied as a biocontrol agent to manage fusarium head blight disease |
| title_short | Minimal impacts on the wheat microbiome when Trichoderma gamsii T6085 is applied as a biocontrol agent to manage fusarium head blight disease |
| title_sort | minimal impacts on the wheat microbiome when trichoderma gamsii t6085 is applied as a biocontrol agent to manage fusarium head blight disease |
| topic | Fusarium graminearum Triticum durum 16S rRNA ITS microbiome fusarium head blight |
| url | https://www.frontiersin.org/articles/10.3389/fmicb.2022.972016/full |
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