Saposhnikovia divaricata root and its major components ameliorate inflammation and altered gut microbial diversity and compositions in DSS-induced colitis
Background: The root of Saposhnikovia divaricata (Turcz.) Schischk is a well-known traditional medicinal plant, containing various bioactive compounds with anti-inflammatory, antioxidant, and analgesic properties. However, no scientific studies have validated its clinical use as an anti-inflammatory...
| Main Authors: | , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Elsevier
2023-12-01
|
| Series: | Integrative Medicine Research |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S221342202300077X |
| _version_ | 1797647602193268736 |
|---|---|
| author | Saruul Erdenebileg Yang-Ju Son Myungsuk Kim Sarangerel Oidovsambuu Kwang Hyun Cha Jaeyoung Kwon Da Seul Jung Chu Won Nho |
| author_facet | Saruul Erdenebileg Yang-Ju Son Myungsuk Kim Sarangerel Oidovsambuu Kwang Hyun Cha Jaeyoung Kwon Da Seul Jung Chu Won Nho |
| author_sort | Saruul Erdenebileg |
| collection | DOAJ |
| description | Background: The root of Saposhnikovia divaricata (Turcz.) Schischk is a well-known traditional medicinal plant, containing various bioactive compounds with anti-inflammatory, antioxidant, and analgesic properties. However, no scientific studies have validated its clinical use as an anti-inflammatory agent against inflammatory bowel disease (IBD). This study aimed to investigate whether the root extract of S. divaricata ameliorates IBD and induces gut microbial alteration, using a RAW 264.7 cell line and a DSS-induced colitis mouse model. Methods: To investigate the anti-inflammatory effects and alleviation of IBD, using a methanol extract of Saposhnikovia divaricata (Turcz.) Schischk. root (MESD), RAW 264.7, murine macrophages and a dextran sodium sulfate (DSS)-induced colitis mouse model were employed. 16S rRNA gene sequencing was conducted to determine the alterations in the gut microbiota of mice with DSS-induced colitis. Results: MESD significantly decreased nitric oxide (NO) and inflammatory cytokine levels in lipopolysaccharide (LPS)-induced RAW 264.7 cells in vitro. Oral administration of MESD reduced the expression of inflammatory cytokines in the colons of mice with DSS-induced colitis. Additionally, MESD inhibited the abundance of Clostridium sensu stricto 1 and enhanced the predicted functional pathways, including l-glutamate degradation VIII (to propanoic acid). Seven compounds with anti-inflammatory properties were isolated from the MESD. Among them, 3′-O-acetylhamaudol and 3′-O-angeloylhamaudol exhibited strong anti-inflammatory effects in vitro. Conclusion: Overall, MESD may be a potential natural product for the treatment of IBD by lowering inflammatory cytokine levels and altering gut microbiota composition. |
| first_indexed | 2024-03-11T15:18:50Z |
| format | Article |
| id | doaj.art-20f23a63c3e74c00ab47c5f5a003666c |
| institution | Directory Open Access Journal |
| issn | 2213-4220 |
| language | English |
| last_indexed | 2024-03-11T15:18:50Z |
| publishDate | 2023-12-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Integrative Medicine Research |
| spelling | doaj.art-20f23a63c3e74c00ab47c5f5a003666c2023-10-29T04:19:45ZengElsevierIntegrative Medicine Research2213-42202023-12-01124100998Saposhnikovia divaricata root and its major components ameliorate inflammation and altered gut microbial diversity and compositions in DSS-induced colitisSaruul Erdenebileg0Yang-Ju Son1Myungsuk Kim2Sarangerel Oidovsambuu3Kwang Hyun Cha4Jaeyoung Kwon5Da Seul Jung6Chu Won Nho7Smart Farm Research Center, Korea Institute of Science and Technology (KIST), Gangneung, South Korea; Division of Bio-Medical Science and Technology, KIST School, University of Science and Technology (UST), Daejeon, South KoreaDepartment of Food and Nutrition, Chung-Ang University, Anseong, South KoreaNatural Product Research Center, Korea Institute of Science and Technology (KIST), Gangneung, South KoreaNatural Product Chemistry Laboratory, Institute of Chemistry and Chemical Technology, Mongolian Academy of Sciences, Ulaanbaatar, MongoliaNatural Product Informatics Research Center, Korea Institute of Science and Technology (KIST), Gangneung, South KoreaNatural Product Informatics Research Center, Korea Institute of Science and Technology (KIST), Gangneung, South KoreaSmart Farm Research Center, Korea Institute of Science and Technology (KIST), Gangneung, South KoreaSmart Farm Research Center, Korea Institute of Science and Technology (KIST), Gangneung, South Korea; Division of Bio-Medical Science and Technology, KIST School, University of Science and Technology (UST), Daejeon, South Korea; Corresponding author at: Smart Farm Research Center, Korea Institute of Science and Technology (KIST), Gangneung, Gangwon-do 25451, South Korea.Background: The root of Saposhnikovia divaricata (Turcz.) Schischk is a well-known traditional medicinal plant, containing various bioactive compounds with anti-inflammatory, antioxidant, and analgesic properties. However, no scientific studies have validated its clinical use as an anti-inflammatory agent against inflammatory bowel disease (IBD). This study aimed to investigate whether the root extract of S. divaricata ameliorates IBD and induces gut microbial alteration, using a RAW 264.7 cell line and a DSS-induced colitis mouse model. Methods: To investigate the anti-inflammatory effects and alleviation of IBD, using a methanol extract of Saposhnikovia divaricata (Turcz.) Schischk. root (MESD), RAW 264.7, murine macrophages and a dextran sodium sulfate (DSS)-induced colitis mouse model were employed. 16S rRNA gene sequencing was conducted to determine the alterations in the gut microbiota of mice with DSS-induced colitis. Results: MESD significantly decreased nitric oxide (NO) and inflammatory cytokine levels in lipopolysaccharide (LPS)-induced RAW 264.7 cells in vitro. Oral administration of MESD reduced the expression of inflammatory cytokines in the colons of mice with DSS-induced colitis. Additionally, MESD inhibited the abundance of Clostridium sensu stricto 1 and enhanced the predicted functional pathways, including l-glutamate degradation VIII (to propanoic acid). Seven compounds with anti-inflammatory properties were isolated from the MESD. Among them, 3′-O-acetylhamaudol and 3′-O-angeloylhamaudol exhibited strong anti-inflammatory effects in vitro. Conclusion: Overall, MESD may be a potential natural product for the treatment of IBD by lowering inflammatory cytokine levels and altering gut microbiota composition.http://www.sciencedirect.com/science/article/pii/S221342202300077XSaposhnikovia divaricataDSS-induced colitisInflammatory bowel diseaseInflammatory responseGut microbiota |
| spellingShingle | Saruul Erdenebileg Yang-Ju Son Myungsuk Kim Sarangerel Oidovsambuu Kwang Hyun Cha Jaeyoung Kwon Da Seul Jung Chu Won Nho Saposhnikovia divaricata root and its major components ameliorate inflammation and altered gut microbial diversity and compositions in DSS-induced colitis Integrative Medicine Research Saposhnikovia divaricata DSS-induced colitis Inflammatory bowel disease Inflammatory response Gut microbiota |
| title | Saposhnikovia divaricata root and its major components ameliorate inflammation and altered gut microbial diversity and compositions in DSS-induced colitis |
| title_full | Saposhnikovia divaricata root and its major components ameliorate inflammation and altered gut microbial diversity and compositions in DSS-induced colitis |
| title_fullStr | Saposhnikovia divaricata root and its major components ameliorate inflammation and altered gut microbial diversity and compositions in DSS-induced colitis |
| title_full_unstemmed | Saposhnikovia divaricata root and its major components ameliorate inflammation and altered gut microbial diversity and compositions in DSS-induced colitis |
| title_short | Saposhnikovia divaricata root and its major components ameliorate inflammation and altered gut microbial diversity and compositions in DSS-induced colitis |
| title_sort | saposhnikovia divaricata root and its major components ameliorate inflammation and altered gut microbial diversity and compositions in dss induced colitis |
| topic | Saposhnikovia divaricata DSS-induced colitis Inflammatory bowel disease Inflammatory response Gut microbiota |
| url | http://www.sciencedirect.com/science/article/pii/S221342202300077X |
| work_keys_str_mv | AT saruulerdenebileg saposhnikoviadivaricatarootanditsmajorcomponentsameliorateinflammationandalteredgutmicrobialdiversityandcompositionsindssinducedcolitis AT yangjuson saposhnikoviadivaricatarootanditsmajorcomponentsameliorateinflammationandalteredgutmicrobialdiversityandcompositionsindssinducedcolitis AT myungsukkim saposhnikoviadivaricatarootanditsmajorcomponentsameliorateinflammationandalteredgutmicrobialdiversityandcompositionsindssinducedcolitis AT sarangereloidovsambuu saposhnikoviadivaricatarootanditsmajorcomponentsameliorateinflammationandalteredgutmicrobialdiversityandcompositionsindssinducedcolitis AT kwanghyuncha saposhnikoviadivaricatarootanditsmajorcomponentsameliorateinflammationandalteredgutmicrobialdiversityandcompositionsindssinducedcolitis AT jaeyoungkwon saposhnikoviadivaricatarootanditsmajorcomponentsameliorateinflammationandalteredgutmicrobialdiversityandcompositionsindssinducedcolitis AT daseuljung saposhnikoviadivaricatarootanditsmajorcomponentsameliorateinflammationandalteredgutmicrobialdiversityandcompositionsindssinducedcolitis AT chuwonnho saposhnikoviadivaricatarootanditsmajorcomponentsameliorateinflammationandalteredgutmicrobialdiversityandcompositionsindssinducedcolitis |