GuttaFlow<sup>®</sup> Bioseal Cytotoxicity Assessment: In Vitro Study
The sealers used for root canal treatment should be biocompatible for the peri-radicular tissues, to evaluate the cytotoxic effects of GuttaFlow<sup>®</sup> bioseal sealer and to compare them with AH26<sup>®</sup> epoxy resin. Culture media were conditioned with the GuttaFlow...
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| Format: | Article |
| Language: | English |
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MDPI AG
2020-09-01
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| Series: | Molecules |
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| Online Access: | https://www.mdpi.com/1420-3049/25/18/4297 |
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| author | Inês Ferreira Mafalda Laranjo Carlos Miguel Marto João Casalta-Lopes Beatriz Serambeque Ana Cristina Gonçalves Ana Bela Sarmento-Ribeiro Eunice Carrilho Maria Filomena Botelho Anabela Baptista Paula Manuel Marques Ferreira |
| author_facet | Inês Ferreira Mafalda Laranjo Carlos Miguel Marto João Casalta-Lopes Beatriz Serambeque Ana Cristina Gonçalves Ana Bela Sarmento-Ribeiro Eunice Carrilho Maria Filomena Botelho Anabela Baptista Paula Manuel Marques Ferreira |
| author_sort | Inês Ferreira |
| collection | DOAJ |
| description | The sealers used for root canal treatment should be biocompatible for the peri-radicular tissues, to evaluate the cytotoxic effects of GuttaFlow<sup>®</sup> bioseal sealer and to compare them with AH26<sup>®</sup> epoxy resin. Culture media were conditioned with the GuttaFlow<sup>®</sup> bioseal and AH26<sup>®</sup> pellets. MDPC-23 odontoblast cell cultures were treated with conditioned medium and serial dilutions. To evaluate the metabolic activity and cellular viability, the MTT and SRB assays were performed. To determine the production of reactive oxygen species, the DHE and DCF-DA probes were used. Cell cycle and cell-death types were assessed by cytometry, and to evaluate the mineralization capacity, the Alizarin Red S coloration was used. Statistical analysis was performed using analysis of variance (ANOVA) when normality was found and Kruskal-Wallis on the opposite case. For the comparison with normality values, the Student <i>t</i>-test was used. Cells exposed to the GuttaFlow<sup>®</sup> bioseal conditioned medium maintained high metabolic activities, except at higher concentrations. Likewise, viability was maintained, but a significant decrease was observed after exposure to the highest concentration (<i>p</i> < 0.001), associated with cell death by late apoptosis and necrosis. When cell cultures were exposed to AH26<sup>®</sup>, metabolic activity was highly compromised, resulting in cell death. An imbalance in the production of peroxides and superoxide anion was observed. GuttaFlow<sup>®</sup> bioseal showed higher biocompatibility than AH26<sup>®</sup>. |
| first_indexed | 2024-03-10T16:12:18Z |
| format | Article |
| id | doaj.art-20f2e9ddc1034f18b653c661c9f30452 |
| institution | Directory Open Access Journal |
| issn | 1420-3049 |
| language | English |
| last_indexed | 2024-03-10T16:12:18Z |
| publishDate | 2020-09-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Molecules |
| spelling | doaj.art-20f2e9ddc1034f18b653c661c9f304522023-11-20T14:19:05ZengMDPI AGMolecules1420-30492020-09-012518429710.3390/molecules25184297GuttaFlow<sup>®</sup> Bioseal Cytotoxicity Assessment: In Vitro StudyInês Ferreira0Mafalda Laranjo1Carlos Miguel Marto2João Casalta-Lopes3Beatriz Serambeque4Ana Cristina Gonçalves5Ana Bela Sarmento-Ribeiro6Eunice Carrilho7Maria Filomena Botelho8Anabela Baptista Paula9Manuel Marques Ferreira10Institute of Endodontics, Faculty of Medicine, University of Coimbra, 3000-075 Coimbra, PortugalInstitute of Biophysics, Faculty of Medicine, University of Coimbra, 3000-548 Coimbra, PortugalInstitute for Clinical and Biomedical Research (iCBR), Area of Environment Genetics and Oncobiology (CIMAGO), Faculty of Medicine, University of Coimbra, 3000-548 Coimbra, PortugalInstitute for Clinical and Biomedical Research (iCBR), Area of Environment Genetics and Oncobiology (CIMAGO), Faculty of Medicine, University of Coimbra, 3000-548 Coimbra, PortugalInstitute of Biophysics, Faculty of Medicine, University of Coimbra, 3000-548 Coimbra, PortugalInstitute for Clinical and Biomedical Research (iCBR), Area of Environment Genetics and Oncobiology (CIMAGO), Faculty of Medicine, University of Coimbra, 3000-548 Coimbra, PortugalInstitute for Clinical and Biomedical Research (iCBR), Area of Environment Genetics and Oncobiology (CIMAGO), Faculty of Medicine, University of Coimbra, 3000-548 Coimbra, PortugalInstitute for Clinical and Biomedical Research (iCBR), Area of Environment Genetics and Oncobiology (CIMAGO), Faculty of Medicine, University of Coimbra, 3000-548 Coimbra, PortugalInstitute of Biophysics, Faculty of Medicine, University of Coimbra, 3000-548 Coimbra, PortugalInstitute for Clinical and Biomedical Research (iCBR), Area of Environment Genetics and Oncobiology (CIMAGO), Faculty of Medicine, University of Coimbra, 3000-548 Coimbra, PortugalInstitute of Endodontics, Faculty of Medicine, University of Coimbra, 3000-075 Coimbra, PortugalThe sealers used for root canal treatment should be biocompatible for the peri-radicular tissues, to evaluate the cytotoxic effects of GuttaFlow<sup>®</sup> bioseal sealer and to compare them with AH26<sup>®</sup> epoxy resin. Culture media were conditioned with the GuttaFlow<sup>®</sup> bioseal and AH26<sup>®</sup> pellets. MDPC-23 odontoblast cell cultures were treated with conditioned medium and serial dilutions. To evaluate the metabolic activity and cellular viability, the MTT and SRB assays were performed. To determine the production of reactive oxygen species, the DHE and DCF-DA probes were used. Cell cycle and cell-death types were assessed by cytometry, and to evaluate the mineralization capacity, the Alizarin Red S coloration was used. Statistical analysis was performed using analysis of variance (ANOVA) when normality was found and Kruskal-Wallis on the opposite case. For the comparison with normality values, the Student <i>t</i>-test was used. Cells exposed to the GuttaFlow<sup>®</sup> bioseal conditioned medium maintained high metabolic activities, except at higher concentrations. Likewise, viability was maintained, but a significant decrease was observed after exposure to the highest concentration (<i>p</i> < 0.001), associated with cell death by late apoptosis and necrosis. When cell cultures were exposed to AH26<sup>®</sup>, metabolic activity was highly compromised, resulting in cell death. An imbalance in the production of peroxides and superoxide anion was observed. GuttaFlow<sup>®</sup> bioseal showed higher biocompatibility than AH26<sup>®</sup>.https://www.mdpi.com/1420-3049/25/18/4297epoxy resinstricalcium phosphatecytotoxicitycell viabilityGuttaFlow<sup>®</sup> biosealAH26<sup>®</sup> |
| spellingShingle | Inês Ferreira Mafalda Laranjo Carlos Miguel Marto João Casalta-Lopes Beatriz Serambeque Ana Cristina Gonçalves Ana Bela Sarmento-Ribeiro Eunice Carrilho Maria Filomena Botelho Anabela Baptista Paula Manuel Marques Ferreira GuttaFlow<sup>®</sup> Bioseal Cytotoxicity Assessment: In Vitro Study Molecules epoxy resins tricalcium phosphate cytotoxicity cell viability GuttaFlow<sup>®</sup> bioseal AH26<sup>®</sup> |
| title | GuttaFlow<sup>®</sup> Bioseal Cytotoxicity Assessment: In Vitro Study |
| title_full | GuttaFlow<sup>®</sup> Bioseal Cytotoxicity Assessment: In Vitro Study |
| title_fullStr | GuttaFlow<sup>®</sup> Bioseal Cytotoxicity Assessment: In Vitro Study |
| title_full_unstemmed | GuttaFlow<sup>®</sup> Bioseal Cytotoxicity Assessment: In Vitro Study |
| title_short | GuttaFlow<sup>®</sup> Bioseal Cytotoxicity Assessment: In Vitro Study |
| title_sort | guttaflow sup r sup bioseal cytotoxicity assessment in vitro study |
| topic | epoxy resins tricalcium phosphate cytotoxicity cell viability GuttaFlow<sup>®</sup> bioseal AH26<sup>®</sup> |
| url | https://www.mdpi.com/1420-3049/25/18/4297 |
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