Optical Behavior of Human Skin Substitutes: Absorbance in the 200–400 nm UV Range
The most recent generation of bioengineered human skin allows for the efficient treatment of patients with severe skin defects. Despite UV sunlight can seriously affect human skin, the optical behavior in the UV range of skin models is still unexplored. In the present study, absorbance and transmitt...
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| Format: | Article |
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MDPI AG
2022-07-01
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| Series: | Biomedicines |
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| Online Access: | https://www.mdpi.com/2227-9059/10/7/1640 |
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| author | Javier Ruiz-López Juan C. Cardona Ingrid Garzón María M. Pérez Miguel Alaminos Jesus Chato-Astrain Ana M. Ionescu |
| author_facet | Javier Ruiz-López Juan C. Cardona Ingrid Garzón María M. Pérez Miguel Alaminos Jesus Chato-Astrain Ana M. Ionescu |
| author_sort | Javier Ruiz-López |
| collection | DOAJ |
| description | The most recent generation of bioengineered human skin allows for the efficient treatment of patients with severe skin defects. Despite UV sunlight can seriously affect human skin, the optical behavior in the UV range of skin models is still unexplored. In the present study, absorbance and transmittance of the UGRSKIN bioartificial skin substitute generated with human skin cells combined with fibrin-agarose biomaterials were evaluated for: UV-C (200–280 nm), -B (280–315 nm), and -A (315–400 nm) spectral range after 7, 14, 21 and 28 days of ex vivo development. The epidermis of the bioartificial skin substitute was able to mature and differentiate in a time-dependent manner, expressing relevant molecules able to absorb most of the incoming UV radiation. Absorbance spectral behavior of the skin substitutes showed similar patterns to control native skin (VAF > 99.4%), with values 0.85–0.90 times lower than control values at 7 and 14- days and 1.05–1.10 times the control values at 21- and 28-days. UV absorbance increased, and UV transmission decreased with culture time, and comparable results to the control were found at 21 and 28 days. These findings support the use of samples corresponding to 21 or 28 days of development for clinical purposes due to their higher histological similarities with native skin, but also because of their absorbance of UV radiation. |
| first_indexed | 2024-03-09T03:40:07Z |
| format | Article |
| id | doaj.art-20fe8485441e4403993383b4d451ce4c |
| institution | Directory Open Access Journal |
| issn | 2227-9059 |
| language | English |
| last_indexed | 2024-03-09T03:40:07Z |
| publishDate | 2022-07-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Biomedicines |
| spelling | doaj.art-20fe8485441e4403993383b4d451ce4c2023-12-03T14:42:08ZengMDPI AGBiomedicines2227-90592022-07-01107164010.3390/biomedicines10071640Optical Behavior of Human Skin Substitutes: Absorbance in the 200–400 nm UV RangeJavier Ruiz-López0Juan C. Cardona1Ingrid Garzón2María M. Pérez3Miguel Alaminos4Jesus Chato-Astrain5Ana M. Ionescu6Department of Optics (Laboratory of Biomaterials Optics), Faculty of Science, University of Granada, E18071 Granada, SpainDepartment of Optics (Laboratory of Biomaterials Optics), Faculty of Science, University of Granada, E18071 Granada, SpainInstituto de Investigación Biosanitaria ibs.GRANADA, E18011 Granada, SpainDepartment of Optics (Laboratory of Biomaterials Optics), Faculty of Science, University of Granada, E18071 Granada, SpainInstituto de Investigación Biosanitaria ibs.GRANADA, E18011 Granada, SpainInstituto de Investigación Biosanitaria ibs.GRANADA, E18011 Granada, SpainDepartment of Optics (Laboratory of Biomaterials Optics), Faculty of Science, University of Granada, E18071 Granada, SpainThe most recent generation of bioengineered human skin allows for the efficient treatment of patients with severe skin defects. Despite UV sunlight can seriously affect human skin, the optical behavior in the UV range of skin models is still unexplored. In the present study, absorbance and transmittance of the UGRSKIN bioartificial skin substitute generated with human skin cells combined with fibrin-agarose biomaterials were evaluated for: UV-C (200–280 nm), -B (280–315 nm), and -A (315–400 nm) spectral range after 7, 14, 21 and 28 days of ex vivo development. The epidermis of the bioartificial skin substitute was able to mature and differentiate in a time-dependent manner, expressing relevant molecules able to absorb most of the incoming UV radiation. Absorbance spectral behavior of the skin substitutes showed similar patterns to control native skin (VAF > 99.4%), with values 0.85–0.90 times lower than control values at 7 and 14- days and 1.05–1.10 times the control values at 21- and 28-days. UV absorbance increased, and UV transmission decreased with culture time, and comparable results to the control were found at 21 and 28 days. These findings support the use of samples corresponding to 21 or 28 days of development for clinical purposes due to their higher histological similarities with native skin, but also because of their absorbance of UV radiation.https://www.mdpi.com/2227-9059/10/7/1640absorptionUV radiationbioengineered skinfibrin-agarose biomaterial |
| spellingShingle | Javier Ruiz-López Juan C. Cardona Ingrid Garzón María M. Pérez Miguel Alaminos Jesus Chato-Astrain Ana M. Ionescu Optical Behavior of Human Skin Substitutes: Absorbance in the 200–400 nm UV Range Biomedicines absorption UV radiation bioengineered skin fibrin-agarose biomaterial |
| title | Optical Behavior of Human Skin Substitutes: Absorbance in the 200–400 nm UV Range |
| title_full | Optical Behavior of Human Skin Substitutes: Absorbance in the 200–400 nm UV Range |
| title_fullStr | Optical Behavior of Human Skin Substitutes: Absorbance in the 200–400 nm UV Range |
| title_full_unstemmed | Optical Behavior of Human Skin Substitutes: Absorbance in the 200–400 nm UV Range |
| title_short | Optical Behavior of Human Skin Substitutes: Absorbance in the 200–400 nm UV Range |
| title_sort | optical behavior of human skin substitutes absorbance in the 200 400 nm uv range |
| topic | absorption UV radiation bioengineered skin fibrin-agarose biomaterial |
| url | https://www.mdpi.com/2227-9059/10/7/1640 |
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