Insight into the Postbiotic Potential of the Autochthonous Bacteriocin-Producing <i>Enterococcus faecium</i> BGZLM1-5 in the Reduction in the Abundance of <i>Listeria monocytogenes</i> ATCC19111 in a Milk Model
This study aimed to explore the probiogenomic characteristics of artisanal bacteriocin-producing <i>Enterococcus faecium</i> BGZLM1-5 and its potential application in reducing <i>Listeria monocytogenes</i> in a milk model. The BGZLM1-5 strain was isolated from raw cow’s milk...
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| Format: | Article |
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MDPI AG
2023-11-01
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| Series: | Microorganisms |
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| Online Access: | https://www.mdpi.com/2076-2607/11/12/2844 |
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| author | Nikola Popović Dušan Stevanović Dušan Radojević Katarina Veljović Jelena Đokić Nataša Golić Amarela Terzić-Vidojević |
| author_facet | Nikola Popović Dušan Stevanović Dušan Radojević Katarina Veljović Jelena Đokić Nataša Golić Amarela Terzić-Vidojević |
| author_sort | Nikola Popović |
| collection | DOAJ |
| description | This study aimed to explore the probiogenomic characteristics of artisanal bacteriocin-producing <i>Enterococcus faecium</i> BGZLM1-5 and its potential application in reducing <i>Listeria monocytogenes</i> in a milk model. The BGZLM1-5 strain was isolated from raw cow’s milk from households in the Zlatar Mountain region. The whole genome sequencing approach and bioinformatics analyses reveal that the strain BGZLM1-5 is non-pathogenic to humans. Bacteriocin-containing supernatant was thermally stable and antimicrobial activity retained 75% of the initial activity compared with that of the control after treatment at 90 °C for 30 min. Antimicrobial activity maintained relative stability at pH 3–11 and retained 62.5% of the initial activity compared with that of the control after treatment at pH 1, 2, and 12. The highest activity of the partially purified bacteriocin was obtained after precipitation at 40% saturation with ammonium sulfate and further purification by mixing with chloroform. Applying 3% and 5% (<i>v/v</i>) of the bacteriocin-containing supernatant and 0.5% (<i>v</i>/<i>v</i>) of the partially purified bacteriocin decreased the viable number of <i>L. monocytogenes</i> ATCC19111 after three days of milk storage by 23.5%, 63.5%, and 58.9%, respectively. |
| first_indexed | 2024-03-08T20:31:39Z |
| format | Article |
| id | doaj.art-21014e7b83c04f8eb8745c477aa3878e |
| institution | Directory Open Access Journal |
| issn | 2076-2607 |
| language | English |
| last_indexed | 2024-03-08T20:31:39Z |
| publishDate | 2023-11-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Microorganisms |
| spelling | doaj.art-21014e7b83c04f8eb8745c477aa3878e2023-12-22T14:25:41ZengMDPI AGMicroorganisms2076-26072023-11-011112284410.3390/microorganisms11122844Insight into the Postbiotic Potential of the Autochthonous Bacteriocin-Producing <i>Enterococcus faecium</i> BGZLM1-5 in the Reduction in the Abundance of <i>Listeria monocytogenes</i> ATCC19111 in a Milk ModelNikola Popović0Dušan Stevanović1Dušan Radojević2Katarina Veljović3Jelena Đokić4Nataša Golić5Amarela Terzić-Vidojević6Institute of Molecular Genetics and Genetic Engineering, University of Belgrade, Vojvode Stepe 444a, 11042 Belgrade, SerbiaInstitute of Molecular Genetics and Genetic Engineering, University of Belgrade, Vojvode Stepe 444a, 11042 Belgrade, SerbiaInstitute of Molecular Genetics and Genetic Engineering, University of Belgrade, Vojvode Stepe 444a, 11042 Belgrade, SerbiaInstitute of Molecular Genetics and Genetic Engineering, University of Belgrade, Vojvode Stepe 444a, 11042 Belgrade, SerbiaInstitute of Molecular Genetics and Genetic Engineering, University of Belgrade, Vojvode Stepe 444a, 11042 Belgrade, SerbiaInstitute of Molecular Genetics and Genetic Engineering, University of Belgrade, Vojvode Stepe 444a, 11042 Belgrade, SerbiaInstitute of Molecular Genetics and Genetic Engineering, University of Belgrade, Vojvode Stepe 444a, 11042 Belgrade, SerbiaThis study aimed to explore the probiogenomic characteristics of artisanal bacteriocin-producing <i>Enterococcus faecium</i> BGZLM1-5 and its potential application in reducing <i>Listeria monocytogenes</i> in a milk model. The BGZLM1-5 strain was isolated from raw cow’s milk from households in the Zlatar Mountain region. The whole genome sequencing approach and bioinformatics analyses reveal that the strain BGZLM1-5 is non-pathogenic to humans. Bacteriocin-containing supernatant was thermally stable and antimicrobial activity retained 75% of the initial activity compared with that of the control after treatment at 90 °C for 30 min. Antimicrobial activity maintained relative stability at pH 3–11 and retained 62.5% of the initial activity compared with that of the control after treatment at pH 1, 2, and 12. The highest activity of the partially purified bacteriocin was obtained after precipitation at 40% saturation with ammonium sulfate and further purification by mixing with chloroform. Applying 3% and 5% (<i>v/v</i>) of the bacteriocin-containing supernatant and 0.5% (<i>v</i>/<i>v</i>) of the partially purified bacteriocin decreased the viable number of <i>L. monocytogenes</i> ATCC19111 after three days of milk storage by 23.5%, 63.5%, and 58.9%, respectively.https://www.mdpi.com/2076-2607/11/12/2844enterococciprobiogenomicsbacteriocinsafety<i>Listeria</i> spp.milk model |
| spellingShingle | Nikola Popović Dušan Stevanović Dušan Radojević Katarina Veljović Jelena Đokić Nataša Golić Amarela Terzić-Vidojević Insight into the Postbiotic Potential of the Autochthonous Bacteriocin-Producing <i>Enterococcus faecium</i> BGZLM1-5 in the Reduction in the Abundance of <i>Listeria monocytogenes</i> ATCC19111 in a Milk Model Microorganisms enterococci probiogenomics bacteriocin safety <i>Listeria</i> spp. milk model |
| title | Insight into the Postbiotic Potential of the Autochthonous Bacteriocin-Producing <i>Enterococcus faecium</i> BGZLM1-5 in the Reduction in the Abundance of <i>Listeria monocytogenes</i> ATCC19111 in a Milk Model |
| title_full | Insight into the Postbiotic Potential of the Autochthonous Bacteriocin-Producing <i>Enterococcus faecium</i> BGZLM1-5 in the Reduction in the Abundance of <i>Listeria monocytogenes</i> ATCC19111 in a Milk Model |
| title_fullStr | Insight into the Postbiotic Potential of the Autochthonous Bacteriocin-Producing <i>Enterococcus faecium</i> BGZLM1-5 in the Reduction in the Abundance of <i>Listeria monocytogenes</i> ATCC19111 in a Milk Model |
| title_full_unstemmed | Insight into the Postbiotic Potential of the Autochthonous Bacteriocin-Producing <i>Enterococcus faecium</i> BGZLM1-5 in the Reduction in the Abundance of <i>Listeria monocytogenes</i> ATCC19111 in a Milk Model |
| title_short | Insight into the Postbiotic Potential of the Autochthonous Bacteriocin-Producing <i>Enterococcus faecium</i> BGZLM1-5 in the Reduction in the Abundance of <i>Listeria monocytogenes</i> ATCC19111 in a Milk Model |
| title_sort | insight into the postbiotic potential of the autochthonous bacteriocin producing i enterococcus faecium i bgzlm1 5 in the reduction in the abundance of i listeria monocytogenes i atcc19111 in a milk model |
| topic | enterococci probiogenomics bacteriocin safety <i>Listeria</i> spp. milk model |
| url | https://www.mdpi.com/2076-2607/11/12/2844 |
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