Investigating the Diversity of <i>Wolbachia</i> across the Spiny Ants (<i>Polyrhachis</i>)

Among insects, <i>Wolbachia</i> is an exceedingly common bacterial endosymbiont with a range of consequences of infection. Despite the frequency of <i>Wolbachia</i> infection, very little is known about this bacteria’s diversity and role within hosts, especially within ant ho...

Full description

Bibliographic Details
Main Authors: Jenna L. Webb, Leland C. Graber, Manuela O. Ramalho, Corrie S. Moreau
Format: Article
Language:English
Published: MDPI AG 2023-03-01
Series:Diversity
Subjects:
Online Access:https://www.mdpi.com/1424-2818/15/3/348
Description
Summary:Among insects, <i>Wolbachia</i> is an exceedingly common bacterial endosymbiont with a range of consequences of infection. Despite the frequency of <i>Wolbachia</i> infection, very little is known about this bacteria’s diversity and role within hosts, especially within ant hosts. In this study, we analyze the occurrence and diversity of <i>Wolbachia</i> across the spiny ants (<i>Polyrhachis</i>), a large and geographically diverse genus. <i>Polyrhachis</i> samples from throughout the host genus’ phylogenetic and biogeographical range were first screened for single infections of <i>Wolbachia</i> using the <i>wsp</i> gene and Sanger sequencing. The multilocus sequence typing (MLST) scheme was then used on these singly infected samples to identify the <i>Wolbachia</i> strains. A <i>Wolbachia</i> phylogeny was inferred from the <i>Polyrhachis</i> samples analyzed in this study as well as other Formicidae MLST profiles from the MLST online database. We hypothesized that three key host factors were impacting <i>Wolbachia</i> diversity within the <i>Polyrhachis</i> genus: biogeography, phylogeny, and species level. The results suggest that the phylogeny and biogeography of <i>Polyrhachis</i> hosts have no impact on <i>Wolbachia</i> diversity; however, species level may have some limited influence. Additionally, <i>Wolbachia</i> strains appear to group according to being either Old World or New World strains. Among the taxa able to form complete MLST allelic profiles, all twenty are seemingly new strains.
ISSN:1424-2818