Bioassay-guided isolation of phenolic apoptosis inducer anticancer agents from Berberis vulgaris fruits
Background: Cancer development is a multi-stage process in which apoptosis plays an important role. Apoptotic agents from natural products like phenolic compounds can be used effectively in the treatment of cancer. Berberis vulgaris L. fruits are a rich source of natural phenolic compounds and have...
| Main Authors: | , , , , , , , |
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| Format: | Article |
| Language: | English |
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Institue of Medicinal Plants, ACECR
2022-09-01
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| Series: | Journal of Medicinal Plants |
| Subjects: | |
| Online Access: | http://jmp.ir/article-1-3336-en.pdf |
| _version_ | 1797773123825696768 |
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| author | Sobhan Ghafourian Ehsan Ghafourian Seyed Mohammad Zarei Fereshteh Jalilian Leila Hosseinzadeh Zulfiqar Ali Yan-Hong Wang Yalda Shokoohinia |
| author_facet | Sobhan Ghafourian Ehsan Ghafourian Seyed Mohammad Zarei Fereshteh Jalilian Leila Hosseinzadeh Zulfiqar Ali Yan-Hong Wang Yalda Shokoohinia |
| author_sort | Sobhan Ghafourian |
| collection | DOAJ |
| description | Background: Cancer development is a multi-stage process in which apoptosis plays an important role. Apoptotic agents from natural products like phenolic compounds can be used effectively in the treatment of cancer. Berberis vulgaris L. fruits are a rich source of natural phenolic compounds and have been evaluated for their apoptotic effects. Objective: this study was carried out to evaluate the apoptotic effect of B. vulgaris fruits in normal and cancerous cells by investigating p53, Bax, and Bcl-2 genes expression and identifying an active compound. Methods: B. vulgaris fruits were extracted with ethanol using maceration method. To yield an active compound, the extract was divided into fractions and subfractions that could be evaluated using chromatography techniques, and the purified compound structure was determined by nuclear magnetic resonance (NMR). The cytotoxic activity of the selected fraction against 3T3 and MCF-7 cell lines along with its effect on the mRNA apoptosis regulating genes like p53, Bax, and Bcl-2 were investigated subsequently. Results: Results showed that B. vulgaris extract increased the mRNA level of p53 and Bax and decreased Bcl-2 mRNA level in cancer cell lines. The structure of the purified compound was determined as the 3-caffeoylquinic acid (chlorogenic acid). Conclusion: B. vulgaris fruit has in vitro antineoplastic activity by stimulating cellular apoptosis via increasing the levels of p53 and Bax proteins and decreasing Bcl-2 and comprises chlorogenic acid as one of its phenolic phytochemicals. |
| first_indexed | 2024-03-12T22:00:45Z |
| format | Article |
| id | doaj.art-210eb7f44e0d4fdfa4f5d15888f0e6c1 |
| institution | Directory Open Access Journal |
| issn | 2717-204X 2717-2058 |
| language | English |
| last_indexed | 2024-03-12T22:00:45Z |
| publishDate | 2022-09-01 |
| publisher | Institue of Medicinal Plants, ACECR |
| record_format | Article |
| series | Journal of Medicinal Plants |
| spelling | doaj.art-210eb7f44e0d4fdfa4f5d15888f0e6c12023-07-25T07:09:26ZengInstitue of Medicinal Plants, ACECRJournal of Medicinal Plants2717-204X2717-20582022-09-0121833546Bioassay-guided isolation of phenolic apoptosis inducer anticancer agents from Berberis vulgaris fruitsSobhan Ghafourian0Ehsan Ghafourian1Seyed Mohammad Zarei2Fereshteh Jalilian3Leila Hosseinzadeh4Zulfiqar Ali5Yan-Hong Wang6Yalda Shokoohinia7 Clinical Microbiology Research Center, Ilam University of Medical Sciences, Ilam, IR Iran Students Research Committee, Kermanshah University of Medical Sciences, Kermanshah, IR Iran Department of Pharmacognosy and Traditional Pharmacy, Faculty of Pharmacy, Baqiyatallah University of Medical Sciences, Tehran, IR Iran Pharmaceutical Sciences Research Center, Health Institute, Kermanshah University of Medical Sciences, Kermanshah, IR Iran Pharmaceutical Sciences Research Center, Health Institute, Kermanshah University of Medical Sciences, Kermanshah, IR Iran National Center for Natural Products Research, University of Mississippi, Oxford, MS, USA National Center for Natural Products Research, University of Mississippi, Oxford, MS, USA Ric Scalzo Botanical Research Institute, Southwest College of Naturopathic Medicine, Tempe, AZ, USA Background: Cancer development is a multi-stage process in which apoptosis plays an important role. Apoptotic agents from natural products like phenolic compounds can be used effectively in the treatment of cancer. Berberis vulgaris L. fruits are a rich source of natural phenolic compounds and have been evaluated for their apoptotic effects. Objective: this study was carried out to evaluate the apoptotic effect of B. vulgaris fruits in normal and cancerous cells by investigating p53, Bax, and Bcl-2 genes expression and identifying an active compound. Methods: B. vulgaris fruits were extracted with ethanol using maceration method. To yield an active compound, the extract was divided into fractions and subfractions that could be evaluated using chromatography techniques, and the purified compound structure was determined by nuclear magnetic resonance (NMR). The cytotoxic activity of the selected fraction against 3T3 and MCF-7 cell lines along with its effect on the mRNA apoptosis regulating genes like p53, Bax, and Bcl-2 were investigated subsequently. Results: Results showed that B. vulgaris extract increased the mRNA level of p53 and Bax and decreased Bcl-2 mRNA level in cancer cell lines. The structure of the purified compound was determined as the 3-caffeoylquinic acid (chlorogenic acid). Conclusion: B. vulgaris fruit has in vitro antineoplastic activity by stimulating cellular apoptosis via increasing the levels of p53 and Bax proteins and decreasing Bcl-2 and comprises chlorogenic acid as one of its phenolic phytochemicals.http://jmp.ir/article-1-3336-en.pdfapoptosisberberis vulgariscancerchlorogenic acidnmr |
| spellingShingle | Sobhan Ghafourian Ehsan Ghafourian Seyed Mohammad Zarei Fereshteh Jalilian Leila Hosseinzadeh Zulfiqar Ali Yan-Hong Wang Yalda Shokoohinia Bioassay-guided isolation of phenolic apoptosis inducer anticancer agents from Berberis vulgaris fruits Journal of Medicinal Plants apoptosis berberis vulgaris cancer chlorogenic acid nmr |
| title | Bioassay-guided isolation of phenolic apoptosis inducer anticancer agents from Berberis vulgaris fruits |
| title_full | Bioassay-guided isolation of phenolic apoptosis inducer anticancer agents from Berberis vulgaris fruits |
| title_fullStr | Bioassay-guided isolation of phenolic apoptosis inducer anticancer agents from Berberis vulgaris fruits |
| title_full_unstemmed | Bioassay-guided isolation of phenolic apoptosis inducer anticancer agents from Berberis vulgaris fruits |
| title_short | Bioassay-guided isolation of phenolic apoptosis inducer anticancer agents from Berberis vulgaris fruits |
| title_sort | bioassay guided isolation of phenolic apoptosis inducer anticancer agents from berberis vulgaris fruits |
| topic | apoptosis berberis vulgaris cancer chlorogenic acid nmr |
| url | http://jmp.ir/article-1-3336-en.pdf |
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