Protective Potential of an Autogenous Vaccine in an Aerogenous Model of <i>Escherichia coli</i> Infection in Broiler Breeders
In poultry, <i>Escherichia coli</i> is a common cause of high-cost infections. Consequently, autogenous vaccines are often used despite limited and conflicting evidence on their effectiveness have been presented. The present study aimed to investigate the efficacy of a commonly used auto...
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| Format: | Article |
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MDPI AG
2021-10-01
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| Series: | Vaccines |
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| Online Access: | https://www.mdpi.com/2076-393X/9/11/1233 |
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| author | Sofie Kromann Rikke Heidemann Olsen Anders Miki Bojesen Henrik Elvang Jensen Ida Thøfner |
| author_facet | Sofie Kromann Rikke Heidemann Olsen Anders Miki Bojesen Henrik Elvang Jensen Ida Thøfner |
| author_sort | Sofie Kromann |
| collection | DOAJ |
| description | In poultry, <i>Escherichia coli</i> is a common cause of high-cost infections. Consequently, autogenous vaccines are often used despite limited and conflicting evidence on their effectiveness have been presented. The present study aimed to investigate the efficacy of a commonly used autogenous vaccine, previously deemed ineffective, in an aerosol model of colibacillosis. Methods: Broiler breeders (<i>n</i> = 47) were randomly allocated to one of four groups (vaccinated and unvaccinated birds receiving an autogenous vaccine or sterile saline intramuscularly) and challenged with either aerosolised <i>E. coli</i> or vehicle at 29 weeks of age. Two days following inoculation, the birds were euthanised, thoroughly necropsied, and samples for bacteriology and histopathology were collected. Results: Vaccinated birds had a significantly lower bacteriology score compared to the unvaccinated group challenged with <i>E. coli</i> (<i>p</i> < 0.01) and a lower overall air sac lesion score (<i>p</i> < 0.05). Overall lung and spleen lesion scores only differed significantly between the unvaccinated <i>E. coli</i> challenged group compared to the vehicle inoculated groups. The overall gross pathology score was 2.8 and 1.95 in the unvaccinated and vaccinated <i>E. coli</i> challenge groups, respectively, whereas the vaccinated vehicle group had a score of 0.9 and the unvaccinated vehicle group a score of 1. Conclusions: A protective effect of an autogenous vaccine was found utilising an aerogenous model of colibacillosis through multiple methods of evaluation. The findings encourage the continued use of autogenous vaccines and underlines the necessity of discriminative experimental models with high predictive validity when evaluating vaccine interventions. |
| first_indexed | 2024-03-10T04:59:53Z |
| format | Article |
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| issn | 2076-393X |
| language | English |
| last_indexed | 2024-03-10T04:59:53Z |
| publishDate | 2021-10-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Vaccines |
| spelling | doaj.art-210f730ed408473393f04b02cbc746cc2023-11-23T01:51:24ZengMDPI AGVaccines2076-393X2021-10-01911123310.3390/vaccines9111233Protective Potential of an Autogenous Vaccine in an Aerogenous Model of <i>Escherichia coli</i> Infection in Broiler BreedersSofie Kromann0Rikke Heidemann Olsen1Anders Miki Bojesen2Henrik Elvang Jensen3Ida Thøfner4Department of Veterinary and Animal Sciences, Faculty of Health and Medical Sciences, University of Copenhagen, 1870 Frederiksberg, DenmarkDepartment of Veterinary and Animal Sciences, Faculty of Health and Medical Sciences, University of Copenhagen, 1870 Frederiksberg, DenmarkDepartment of Veterinary and Animal Sciences, Faculty of Health and Medical Sciences, University of Copenhagen, 1870 Frederiksberg, DenmarkDepartment of Veterinary and Animal Sciences, Faculty of Health and Medical Sciences, University of Copenhagen, 1870 Frederiksberg, DenmarkDepartment of Veterinary and Animal Sciences, Faculty of Health and Medical Sciences, University of Copenhagen, 1870 Frederiksberg, DenmarkIn poultry, <i>Escherichia coli</i> is a common cause of high-cost infections. Consequently, autogenous vaccines are often used despite limited and conflicting evidence on their effectiveness have been presented. The present study aimed to investigate the efficacy of a commonly used autogenous vaccine, previously deemed ineffective, in an aerosol model of colibacillosis. Methods: Broiler breeders (<i>n</i> = 47) were randomly allocated to one of four groups (vaccinated and unvaccinated birds receiving an autogenous vaccine or sterile saline intramuscularly) and challenged with either aerosolised <i>E. coli</i> or vehicle at 29 weeks of age. Two days following inoculation, the birds were euthanised, thoroughly necropsied, and samples for bacteriology and histopathology were collected. Results: Vaccinated birds had a significantly lower bacteriology score compared to the unvaccinated group challenged with <i>E. coli</i> (<i>p</i> < 0.01) and a lower overall air sac lesion score (<i>p</i> < 0.05). Overall lung and spleen lesion scores only differed significantly between the unvaccinated <i>E. coli</i> challenged group compared to the vehicle inoculated groups. The overall gross pathology score was 2.8 and 1.95 in the unvaccinated and vaccinated <i>E. coli</i> challenge groups, respectively, whereas the vaccinated vehicle group had a score of 0.9 and the unvaccinated vehicle group a score of 1. Conclusions: A protective effect of an autogenous vaccine was found utilising an aerogenous model of colibacillosis through multiple methods of evaluation. The findings encourage the continued use of autogenous vaccines and underlines the necessity of discriminative experimental models with high predictive validity when evaluating vaccine interventions.https://www.mdpi.com/2076-393X/9/11/1233APECcolibacillosischallenge studypoultry diseasedisease preventionbacterin vaccine |
| spellingShingle | Sofie Kromann Rikke Heidemann Olsen Anders Miki Bojesen Henrik Elvang Jensen Ida Thøfner Protective Potential of an Autogenous Vaccine in an Aerogenous Model of <i>Escherichia coli</i> Infection in Broiler Breeders Vaccines APEC colibacillosis challenge study poultry disease disease prevention bacterin vaccine |
| title | Protective Potential of an Autogenous Vaccine in an Aerogenous Model of <i>Escherichia coli</i> Infection in Broiler Breeders |
| title_full | Protective Potential of an Autogenous Vaccine in an Aerogenous Model of <i>Escherichia coli</i> Infection in Broiler Breeders |
| title_fullStr | Protective Potential of an Autogenous Vaccine in an Aerogenous Model of <i>Escherichia coli</i> Infection in Broiler Breeders |
| title_full_unstemmed | Protective Potential of an Autogenous Vaccine in an Aerogenous Model of <i>Escherichia coli</i> Infection in Broiler Breeders |
| title_short | Protective Potential of an Autogenous Vaccine in an Aerogenous Model of <i>Escherichia coli</i> Infection in Broiler Breeders |
| title_sort | protective potential of an autogenous vaccine in an aerogenous model of i escherichia coli i infection in broiler breeders |
| topic | APEC colibacillosis challenge study poultry disease disease prevention bacterin vaccine |
| url | https://www.mdpi.com/2076-393X/9/11/1233 |
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