High-level production of pullulan and its biosynthesis regulation in Aureobasidium pullulans BL06
Pullulan has many potential applications in the food, pharmaceutical, cosmetic and environmental industries. However, the yield and molecular properties of pullulan produced by various strains still need to be promoted to fit the application needs. A novel yeast-like strain Aureobasidium pullulans B...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2023-01-01
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| Series: | Frontiers in Bioengineering and Biotechnology |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fbioe.2023.1131875/full |
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| author | Shuyu Chen Hongchen Zheng Hongchen Zheng Hongchen Zheng Hongchen Zheng Jiaqi Gao Jiaqi Gao Jiaqi Gao Hui Song Hui Song Hui Song Wenqin Bai Wenqin Bai Wenqin Bai Wenqin Bai |
| author_facet | Shuyu Chen Hongchen Zheng Hongchen Zheng Hongchen Zheng Hongchen Zheng Jiaqi Gao Jiaqi Gao Jiaqi Gao Hui Song Hui Song Hui Song Wenqin Bai Wenqin Bai Wenqin Bai Wenqin Bai |
| author_sort | Shuyu Chen |
| collection | DOAJ |
| description | Pullulan has many potential applications in the food, pharmaceutical, cosmetic and environmental industries. However, the yield and molecular properties of pullulan produced by various strains still need to be promoted to fit the application needs. A novel yeast-like strain Aureobasidium pullulans BL06 producing high molecular weight (Mw) pullulan (3.3 × 106 Da) was isolated and identified in this study. The remarkable Mw of pullulan produced by A. pullulans BL06 was the highest level ever reported thus far. To further regulate the biosynthesis of pullulan in A. pullulans BL06, three gene knockout strains A. pullulans BL06 ΔPMAs, A. pullulans BL06 Δmel, and A. pullulans BL06 ΔPMAsΔmel, were constructed. The results showed that A. pullulans BL06 ΔPMAs could produce 140.2 g/L of moderate Mw (1.3 × 105 Da) pullulan after 120 h of fermentation. The highest yield level of pullulan to date could vastly reduce its production cost and expand its application scope and potential. The application experiments in food preservation showed that the moderate-Mw pullulan obtained in this work could reduce the weight loss of celery cabbages and mangos by 12.5% and 22%, respectively. Thus, the novel strains A. pullulans BL06 and A. pullulans BL06 ΔPMAs possessed unlimited development prospects in pullulan production at various Mw ranges and pullulan applications in multiple fields. |
| first_indexed | 2024-04-10T20:14:11Z |
| format | Article |
| id | doaj.art-21118962fe304160a7ab11ab8cb310bd |
| institution | Directory Open Access Journal |
| issn | 2296-4185 |
| language | English |
| last_indexed | 2024-04-10T20:14:11Z |
| publishDate | 2023-01-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Bioengineering and Biotechnology |
| spelling | doaj.art-21118962fe304160a7ab11ab8cb310bd2023-01-26T07:41:36ZengFrontiers Media S.A.Frontiers in Bioengineering and Biotechnology2296-41852023-01-011110.3389/fbioe.2023.11318751131875High-level production of pullulan and its biosynthesis regulation in Aureobasidium pullulans BL06Shuyu Chen0Hongchen Zheng1Hongchen Zheng2Hongchen Zheng3Hongchen Zheng4Jiaqi Gao5Jiaqi Gao6Jiaqi Gao7Hui Song8Hui Song9Hui Song10Wenqin Bai11Wenqin Bai12Wenqin Bai13Wenqin Bai14Colleg of Biotechnology, Tianjin University of Science Technology, Tianjin, ChinaCAS Key Laboratory of Systems Microbial Biotechnology, Tianjin Institute of Industrial Biotechnology, Chinese Academy of Sciences, Tianjin, ChinaNational Center of Technology Innovation for Synthetic Biology, Tianjin, ChinaIndustrial Enzymes National Engineering Research Center, Tianjin Institute of Industrial Biotechnology, Chinese Academy of Sciences, Tianjin, ChinaKey Laboratory of Engineering Biology for Low-carbon Manufacturing, Tianjin Institute of Industrial Biotechnology, Chinese Academy of Sciences, Tianjin, ChinaCAS Key Laboratory of Systems Microbial Biotechnology, Tianjin Institute of Industrial Biotechnology, Chinese Academy of Sciences, Tianjin, ChinaNational Center of Technology Innovation for Synthetic Biology, Tianjin, ChinaIndustrial Enzymes National Engineering Research Center, Tianjin Institute of Industrial Biotechnology, Chinese Academy of Sciences, Tianjin, ChinaCAS Key Laboratory of Systems Microbial Biotechnology, Tianjin Institute of Industrial Biotechnology, Chinese Academy of Sciences, Tianjin, ChinaNational Center of Technology Innovation for Synthetic Biology, Tianjin, ChinaIndustrial Enzymes National Engineering Research Center, Tianjin Institute of Industrial Biotechnology, Chinese Academy of Sciences, Tianjin, ChinaCAS Key Laboratory of Systems Microbial Biotechnology, Tianjin Institute of Industrial Biotechnology, Chinese Academy of Sciences, Tianjin, ChinaNational Center of Technology Innovation for Synthetic Biology, Tianjin, ChinaIndustrial Enzymes National Engineering Research Center, Tianjin Institute of Industrial Biotechnology, Chinese Academy of Sciences, Tianjin, ChinaKey Laboratory of Engineering Biology for Low-carbon Manufacturing, Tianjin Institute of Industrial Biotechnology, Chinese Academy of Sciences, Tianjin, ChinaPullulan has many potential applications in the food, pharmaceutical, cosmetic and environmental industries. However, the yield and molecular properties of pullulan produced by various strains still need to be promoted to fit the application needs. A novel yeast-like strain Aureobasidium pullulans BL06 producing high molecular weight (Mw) pullulan (3.3 × 106 Da) was isolated and identified in this study. The remarkable Mw of pullulan produced by A. pullulans BL06 was the highest level ever reported thus far. To further regulate the biosynthesis of pullulan in A. pullulans BL06, three gene knockout strains A. pullulans BL06 ΔPMAs, A. pullulans BL06 Δmel, and A. pullulans BL06 ΔPMAsΔmel, were constructed. The results showed that A. pullulans BL06 ΔPMAs could produce 140.2 g/L of moderate Mw (1.3 × 105 Da) pullulan after 120 h of fermentation. The highest yield level of pullulan to date could vastly reduce its production cost and expand its application scope and potential. The application experiments in food preservation showed that the moderate-Mw pullulan obtained in this work could reduce the weight loss of celery cabbages and mangos by 12.5% and 22%, respectively. Thus, the novel strains A. pullulans BL06 and A. pullulans BL06 ΔPMAs possessed unlimited development prospects in pullulan production at various Mw ranges and pullulan applications in multiple fields.https://www.frontiersin.org/articles/10.3389/fbioe.2023.1131875/fullpullulanaureobasidium pullulansbiosynthesishigh-level productionhigh molecular weightmoderate molecular weight |
| spellingShingle | Shuyu Chen Hongchen Zheng Hongchen Zheng Hongchen Zheng Hongchen Zheng Jiaqi Gao Jiaqi Gao Jiaqi Gao Hui Song Hui Song Hui Song Wenqin Bai Wenqin Bai Wenqin Bai Wenqin Bai High-level production of pullulan and its biosynthesis regulation in Aureobasidium pullulans BL06 Frontiers in Bioengineering and Biotechnology pullulan aureobasidium pullulans biosynthesis high-level production high molecular weight moderate molecular weight |
| title | High-level production of pullulan and its biosynthesis regulation in Aureobasidium pullulans BL06 |
| title_full | High-level production of pullulan and its biosynthesis regulation in Aureobasidium pullulans BL06 |
| title_fullStr | High-level production of pullulan and its biosynthesis regulation in Aureobasidium pullulans BL06 |
| title_full_unstemmed | High-level production of pullulan and its biosynthesis regulation in Aureobasidium pullulans BL06 |
| title_short | High-level production of pullulan and its biosynthesis regulation in Aureobasidium pullulans BL06 |
| title_sort | high level production of pullulan and its biosynthesis regulation in aureobasidium pullulans bl06 |
| topic | pullulan aureobasidium pullulans biosynthesis high-level production high molecular weight moderate molecular weight |
| url | https://www.frontiersin.org/articles/10.3389/fbioe.2023.1131875/full |
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