Gut microbiota and intestinal barrier function in subjects with cognitive impairments: a cross-sectional study
BackgroundGut-brain axis might play an important role in cognitive impairments by various diseases including Alzheimer’s disease (AD).ObjectiveTo investigate the differences in gut microbial composition, intestinal barrier function, and systemic inflammation in patients with AD or mild cognitive imp...
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2023-06-01
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| Series: | Frontiers in Aging Neuroscience |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fnagi.2023.1174599/full |
| _version_ | 1797809896945614848 |
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| author | Ying Pei Ying Pei Yan Lu HuiZi Li ChengYing Jiang Lei Wang |
| author_facet | Ying Pei Ying Pei Yan Lu HuiZi Li ChengYing Jiang Lei Wang |
| author_sort | Ying Pei |
| collection | DOAJ |
| description | BackgroundGut-brain axis might play an important role in cognitive impairments by various diseases including Alzheimer’s disease (AD).ObjectiveTo investigate the differences in gut microbial composition, intestinal barrier function, and systemic inflammation in patients with AD or mild cognitive impairment (MCI), and normal control (NC) cases.MethodsA total of 118 subjects (45 AD, 38 MCI, and 35 NC) were recruited. Cognitive function was assessed using Mini-Mental State Examination (MMSE), and Montreal Cognitive Assessment Scale (MoCA). Functional ability was assessed using Activity of Daily Living Scale (ADL). The composition of gut microbiome was examined by 16S rRNA high-throughput sequencing. Phylogenetic Investigation of Communities by Reconstruction of Unobserved States (PICRUSt) was used to predict functional transfer of gut microbiota. Gut barrier dysfunction was evaluated by measuring the levels of diamine oxidase (DAO), D-lactic acid (DA), and endotoxin (ET). The serum high-sensitivity C-reactive protein (hs-CRP) level was used to indicate systemic inflammation.ResultsCompared with normal controls, patients with cognitive impairments (AD and MCI) had lower abundance of Dorea and higher levels of DAO, DA, and ET. Kyoto Encyclopedia of Genes and Genomes (KEGG) results showed that the pathways related to glycan biosynthesis and metabolism increased in MCI patients, while the ones related to membrane transport decreased. The abundance of Bacteroides and Faecalibacterium was negatively correlated with the content of ET, and positively correlated with the scores of MMSE and MoCA. The hs-CRP levels were similar among the three groups. A significant negative correlation was observed between the severity of gut barrier dysfunction and cognitive function.ConclusionCognitive impairments might be associated with gut microbial dysbiosis and intestinal barrier dysfunction. |
| first_indexed | 2024-03-13T06:59:50Z |
| format | Article |
| id | doaj.art-2111c757640d45648e440addfaea5426 |
| institution | Directory Open Access Journal |
| issn | 1663-4365 |
| language | English |
| last_indexed | 2024-03-13T06:59:50Z |
| publishDate | 2023-06-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Aging Neuroscience |
| spelling | doaj.art-2111c757640d45648e440addfaea54262023-06-07T04:26:53ZengFrontiers Media S.A.Frontiers in Aging Neuroscience1663-43652023-06-011510.3389/fnagi.2023.11745991174599Gut microbiota and intestinal barrier function in subjects with cognitive impairments: a cross-sectional studyYing Pei0Ying Pei1Yan Lu2HuiZi Li3ChengYing Jiang4Lei Wang5Postgraduate Union Training Base of Jinzhou Medical University, PLA Rocket Force Characteristic Medical Center, Beijing, ChinaDepartment of Neurology, PLA Rocket Force Characteristic Medical Center, Beijing, ChinaDepartment of Neurology, PLA Rocket Force Characteristic Medical Center, Beijing, ChinaDepartment of Nutrition, PLA Rocket Force Characteristic Medical Center, Beijing, ChinaInstitute of Microbiology, Chinese Academy of Sciences, Beijing, ChinaDepartment of Neurology, PLA Rocket Force Characteristic Medical Center, Beijing, ChinaBackgroundGut-brain axis might play an important role in cognitive impairments by various diseases including Alzheimer’s disease (AD).ObjectiveTo investigate the differences in gut microbial composition, intestinal barrier function, and systemic inflammation in patients with AD or mild cognitive impairment (MCI), and normal control (NC) cases.MethodsA total of 118 subjects (45 AD, 38 MCI, and 35 NC) were recruited. Cognitive function was assessed using Mini-Mental State Examination (MMSE), and Montreal Cognitive Assessment Scale (MoCA). Functional ability was assessed using Activity of Daily Living Scale (ADL). The composition of gut microbiome was examined by 16S rRNA high-throughput sequencing. Phylogenetic Investigation of Communities by Reconstruction of Unobserved States (PICRUSt) was used to predict functional transfer of gut microbiota. Gut barrier dysfunction was evaluated by measuring the levels of diamine oxidase (DAO), D-lactic acid (DA), and endotoxin (ET). The serum high-sensitivity C-reactive protein (hs-CRP) level was used to indicate systemic inflammation.ResultsCompared with normal controls, patients with cognitive impairments (AD and MCI) had lower abundance of Dorea and higher levels of DAO, DA, and ET. Kyoto Encyclopedia of Genes and Genomes (KEGG) results showed that the pathways related to glycan biosynthesis and metabolism increased in MCI patients, while the ones related to membrane transport decreased. The abundance of Bacteroides and Faecalibacterium was negatively correlated with the content of ET, and positively correlated with the scores of MMSE and MoCA. The hs-CRP levels were similar among the three groups. A significant negative correlation was observed between the severity of gut barrier dysfunction and cognitive function.ConclusionCognitive impairments might be associated with gut microbial dysbiosis and intestinal barrier dysfunction.https://www.frontiersin.org/articles/10.3389/fnagi.2023.1174599/fullgut microbiotaintestinal barriercognitive impairmentgut-brain axisAlzheimer’s disease |
| spellingShingle | Ying Pei Ying Pei Yan Lu HuiZi Li ChengYing Jiang Lei Wang Gut microbiota and intestinal barrier function in subjects with cognitive impairments: a cross-sectional study Frontiers in Aging Neuroscience gut microbiota intestinal barrier cognitive impairment gut-brain axis Alzheimer’s disease |
| title | Gut microbiota and intestinal barrier function in subjects with cognitive impairments: a cross-sectional study |
| title_full | Gut microbiota and intestinal barrier function in subjects with cognitive impairments: a cross-sectional study |
| title_fullStr | Gut microbiota and intestinal barrier function in subjects with cognitive impairments: a cross-sectional study |
| title_full_unstemmed | Gut microbiota and intestinal barrier function in subjects with cognitive impairments: a cross-sectional study |
| title_short | Gut microbiota and intestinal barrier function in subjects with cognitive impairments: a cross-sectional study |
| title_sort | gut microbiota and intestinal barrier function in subjects with cognitive impairments a cross sectional study |
| topic | gut microbiota intestinal barrier cognitive impairment gut-brain axis Alzheimer’s disease |
| url | https://www.frontiersin.org/articles/10.3389/fnagi.2023.1174599/full |
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