Screening Spring Wheat Genotypes for <i>TaDreb-B1</i> and <i>Fehw3</i> Genes under Severe Drought Stress at the Germination Stage Using KASP Technology
Drought stress is a major yield-limiting factor throughout the world in wheat (<i>Triticum aestivum</i> L.), causing losses of up to 80% of the total yield. The identification of factors affecting drought stress tolerance in the seedling stage is especially important to increase adaptati...
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MDPI AG
2023-01-01
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| author | Elsayed A. Mohamed Asmaa A. M. Ahmed Matías Schierenbeck Mohamed Y. Hussein P. Stephen Baenziger Andreas Börner Ahmed Sallam |
| author_facet | Elsayed A. Mohamed Asmaa A. M. Ahmed Matías Schierenbeck Mohamed Y. Hussein P. Stephen Baenziger Andreas Börner Ahmed Sallam |
| author_sort | Elsayed A. Mohamed |
| collection | DOAJ |
| description | Drought stress is a major yield-limiting factor throughout the world in wheat (<i>Triticum aestivum</i> L.), causing losses of up to 80% of the total yield. The identification of factors affecting drought stress tolerance in the seedling stage is especially important to increase adaptation and accelerate the grain yield potential. In the current study, 41 spring wheat genotypes were tested for their tolerance to drought at the germination stage under two different polyethylene glycol concentrations (PEG) of 25% and 30%. For this purpose, twenty seedlings from each genotype were evaluated in triplicate with a randomized complete block design (RCBD) in a controlled growth chamber. The following nine parameters were recorded: germination pace (GP), germination percentage (G%), number of roots (NR), shoot length (SL), root length (RL), shoot–root length ratio (SRR), fresh biomass weight (FBW), dry biomass weight (DBW), and water content (WC). An analysis of variance (ANOVA) revealed highly significant differences (<i>p</i> < 0.01) among the genotypes, treatments (PEG25%, PEG30%) and genotypes × treatment interaction, for all traits. The broad-sense heritability (H<sup>2</sup>) estimates were very high in both concentrations. They ranged from 89.4 to 98.9% under PEG25% and from 70.8 to 98.7% under PEG30%. Citr15314 (Afghanistan) was among the best performing genotypes under both concentrations for most of the germination traits. Two KASP markers for <i>TaDreb-B1</i> and <i>Fehw3</i> genes were used to screen all genotypes and to study the effect of these on drought tolerance at the germination stage. All genotypes with <i>Fehw3</i> (only) showed a better performance for most traits under both concentrations compared to other genotypes having <i>TaDreb-B1</i> or having both genes. To our knowledge, this work is the first report showing the effect of the two genes on germination traits under severe drought stress conditions. |
| first_indexed | 2024-03-11T08:46:37Z |
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| institution | Directory Open Access Journal |
| issn | 2073-4425 |
| language | English |
| last_indexed | 2024-03-11T08:46:37Z |
| publishDate | 2023-01-01 |
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| spelling | doaj.art-2122397724ca4f9eb62b4ac1c1bd4c322023-11-16T20:42:05ZengMDPI AGGenes2073-44252023-01-0114237310.3390/genes14020373Screening Spring Wheat Genotypes for <i>TaDreb-B1</i> and <i>Fehw3</i> Genes under Severe Drought Stress at the Germination Stage Using KASP TechnologyElsayed A. Mohamed0Asmaa A. M. Ahmed1Matías Schierenbeck2Mohamed Y. Hussein3P. Stephen Baenziger4Andreas Börner5Ahmed Sallam6Department of Genetics, Faculty of Agriculture, Assiut University, Assiut 71526, EgyptDepartment of Genetics, Faculty of Agriculture, Assiut University, Assiut 71526, EgyptResources Genetics and Reproduction, Department Genebank, Leibniz Institute of Plant Genetics and Crop Plant Research (IPK), Corrensstr. 3, OT Gatersleben, D-06466 Stadt Seeland, GermanyDepartment of Genetics, Faculty of Agriculture, Assiut University, Assiut 71526, EgyptDepartment of Agronomy and Horticulture, University of Nebraska–Lincoln, Lincoln, NE 68583, USAResources Genetics and Reproduction, Department Genebank, Leibniz Institute of Plant Genetics and Crop Plant Research (IPK), Corrensstr. 3, OT Gatersleben, D-06466 Stadt Seeland, GermanyDepartment of Genetics, Faculty of Agriculture, Assiut University, Assiut 71526, EgyptDrought stress is a major yield-limiting factor throughout the world in wheat (<i>Triticum aestivum</i> L.), causing losses of up to 80% of the total yield. The identification of factors affecting drought stress tolerance in the seedling stage is especially important to increase adaptation and accelerate the grain yield potential. In the current study, 41 spring wheat genotypes were tested for their tolerance to drought at the germination stage under two different polyethylene glycol concentrations (PEG) of 25% and 30%. For this purpose, twenty seedlings from each genotype were evaluated in triplicate with a randomized complete block design (RCBD) in a controlled growth chamber. The following nine parameters were recorded: germination pace (GP), germination percentage (G%), number of roots (NR), shoot length (SL), root length (RL), shoot–root length ratio (SRR), fresh biomass weight (FBW), dry biomass weight (DBW), and water content (WC). An analysis of variance (ANOVA) revealed highly significant differences (<i>p</i> < 0.01) among the genotypes, treatments (PEG25%, PEG30%) and genotypes × treatment interaction, for all traits. The broad-sense heritability (H<sup>2</sup>) estimates were very high in both concentrations. They ranged from 89.4 to 98.9% under PEG25% and from 70.8 to 98.7% under PEG30%. Citr15314 (Afghanistan) was among the best performing genotypes under both concentrations for most of the germination traits. Two KASP markers for <i>TaDreb-B1</i> and <i>Fehw3</i> genes were used to screen all genotypes and to study the effect of these on drought tolerance at the germination stage. All genotypes with <i>Fehw3</i> (only) showed a better performance for most traits under both concentrations compared to other genotypes having <i>TaDreb-B1</i> or having both genes. To our knowledge, this work is the first report showing the effect of the two genes on germination traits under severe drought stress conditions.https://www.mdpi.com/2073-4425/14/2/373water deficitgenetic variationgermination traits<i>Triticum aestivum</i> L.PEGKASP |
| spellingShingle | Elsayed A. Mohamed Asmaa A. M. Ahmed Matías Schierenbeck Mohamed Y. Hussein P. Stephen Baenziger Andreas Börner Ahmed Sallam Screening Spring Wheat Genotypes for <i>TaDreb-B1</i> and <i>Fehw3</i> Genes under Severe Drought Stress at the Germination Stage Using KASP Technology Genes water deficit genetic variation germination traits <i>Triticum aestivum</i> L. PEG KASP |
| title | Screening Spring Wheat Genotypes for <i>TaDreb-B1</i> and <i>Fehw3</i> Genes under Severe Drought Stress at the Germination Stage Using KASP Technology |
| title_full | Screening Spring Wheat Genotypes for <i>TaDreb-B1</i> and <i>Fehw3</i> Genes under Severe Drought Stress at the Germination Stage Using KASP Technology |
| title_fullStr | Screening Spring Wheat Genotypes for <i>TaDreb-B1</i> and <i>Fehw3</i> Genes under Severe Drought Stress at the Germination Stage Using KASP Technology |
| title_full_unstemmed | Screening Spring Wheat Genotypes for <i>TaDreb-B1</i> and <i>Fehw3</i> Genes under Severe Drought Stress at the Germination Stage Using KASP Technology |
| title_short | Screening Spring Wheat Genotypes for <i>TaDreb-B1</i> and <i>Fehw3</i> Genes under Severe Drought Stress at the Germination Stage Using KASP Technology |
| title_sort | screening spring wheat genotypes for i tadreb b1 i and i fehw3 i genes under severe drought stress at the germination stage using kasp technology |
| topic | water deficit genetic variation germination traits <i>Triticum aestivum</i> L. PEG KASP |
| url | https://www.mdpi.com/2073-4425/14/2/373 |
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