Amygdalin inhibits angiogenesis in the cultured endothelial cells of diabetic rats
Background: Angiogenesis contributes to different physiological and pathological conditions. The aim of this study was to investigate for the first time the antiangiogenic effects of amygdalin on the cultured endothelial cells of diabetic rats. Materials and Methods: A total of 20 streptozotocin-ind...
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| Format: | Article |
| Language: | English |
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Wolters Kluwer Medknow Publications
2012-01-01
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| Series: | Indian Journal of Pathology and Microbiology |
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| Online Access: | http://www.ijpmonline.org/article.asp?issn=0377-4929;year=2012;volume=55;issue=2;spage=211;epage=214;aulast=Mirmiranpour |
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| author | Hossein Mirmiranpour Shahnaz Khaghani Ali Zandieh O Omid Khalilzadeh Siavash Gerayesh-Nejad Afsaneh Morteza Alireza Esteghamati |
| author_facet | Hossein Mirmiranpour Shahnaz Khaghani Ali Zandieh O Omid Khalilzadeh Siavash Gerayesh-Nejad Afsaneh Morteza Alireza Esteghamati |
| author_sort | Hossein Mirmiranpour |
| collection | DOAJ |
| description | Background: Angiogenesis contributes to different physiological and pathological conditions. The aim of this study was to investigate for the first time the antiangiogenic effects of amygdalin on the cultured endothelial cells of diabetic rats. Materials and Methods: A total of 20 streptozotocin-induced diabetic rats were divided into two equal groups of control and amygdalin-treated animals. Eight weeks after the induction of diabetes, amygdalin was injected intraperitoneally (3 mg/kg) to the rats of the treatment group. One day later, rats were sacrificed; the aortic arteries were excised and cut as 2 mm rings. Each aortic ring was incubated in a cell-culture well for 7 days. The process of angiogenesis was monitored by counting the number of microvessels and primary microtubules in each well. Results: Optic microscopy showed proliferation and migration of new endothelial cells to the fibrin gels. The endothelial cells produced primary microtubules which gradually made several branches and finally made a vascular matrix. The number of the primary microtubules and microvessels were significantly lower in the amygdalin-treated vs. control group (P < 0.01). Conclusion: Therefore, amygdalin exerts inhibitory effects on angiogenesis in aortic rings of diabetic rats and may pave a new way for treatment of unfavorable angiogenic conditions. |
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| format | Article |
| id | doaj.art-2123844be4104a159ff5f25394dafb40 |
| institution | Directory Open Access Journal |
| issn | 0377-4929 |
| language | English |
| last_indexed | 2024-12-21T15:54:40Z |
| publishDate | 2012-01-01 |
| publisher | Wolters Kluwer Medknow Publications |
| record_format | Article |
| series | Indian Journal of Pathology and Microbiology |
| spelling | doaj.art-2123844be4104a159ff5f25394dafb402022-12-21T18:58:08ZengWolters Kluwer Medknow PublicationsIndian Journal of Pathology and Microbiology0377-49292012-01-0155221121410.4103/0377-4929.97874Amygdalin inhibits angiogenesis in the cultured endothelial cells of diabetic ratsHossein MirmiranpourShahnaz KhaghaniAli ZandiehO Omid KhalilzadehSiavash Gerayesh-NejadAfsaneh MortezaAlireza EsteghamatiBackground: Angiogenesis contributes to different physiological and pathological conditions. The aim of this study was to investigate for the first time the antiangiogenic effects of amygdalin on the cultured endothelial cells of diabetic rats. Materials and Methods: A total of 20 streptozotocin-induced diabetic rats were divided into two equal groups of control and amygdalin-treated animals. Eight weeks after the induction of diabetes, amygdalin was injected intraperitoneally (3 mg/kg) to the rats of the treatment group. One day later, rats were sacrificed; the aortic arteries were excised and cut as 2 mm rings. Each aortic ring was incubated in a cell-culture well for 7 days. The process of angiogenesis was monitored by counting the number of microvessels and primary microtubules in each well. Results: Optic microscopy showed proliferation and migration of new endothelial cells to the fibrin gels. The endothelial cells produced primary microtubules which gradually made several branches and finally made a vascular matrix. The number of the primary microtubules and microvessels were significantly lower in the amygdalin-treated vs. control group (P < 0.01). Conclusion: Therefore, amygdalin exerts inhibitory effects on angiogenesis in aortic rings of diabetic rats and may pave a new way for treatment of unfavorable angiogenic conditions.http://www.ijpmonline.org/article.asp?issn=0377-4929;year=2012;volume=55;issue=2;spage=211;epage=214;aulast=MirmiranpourAmygdalinangiogenesisdiabetesendothelial cellsrat′s aortic rings |
| spellingShingle | Hossein Mirmiranpour Shahnaz Khaghani Ali Zandieh O Omid Khalilzadeh Siavash Gerayesh-Nejad Afsaneh Morteza Alireza Esteghamati Amygdalin inhibits angiogenesis in the cultured endothelial cells of diabetic rats Indian Journal of Pathology and Microbiology Amygdalin angiogenesis diabetes endothelial cells rat′s aortic rings |
| title | Amygdalin inhibits angiogenesis in the cultured endothelial cells of diabetic rats |
| title_full | Amygdalin inhibits angiogenesis in the cultured endothelial cells of diabetic rats |
| title_fullStr | Amygdalin inhibits angiogenesis in the cultured endothelial cells of diabetic rats |
| title_full_unstemmed | Amygdalin inhibits angiogenesis in the cultured endothelial cells of diabetic rats |
| title_short | Amygdalin inhibits angiogenesis in the cultured endothelial cells of diabetic rats |
| title_sort | amygdalin inhibits angiogenesis in the cultured endothelial cells of diabetic rats |
| topic | Amygdalin angiogenesis diabetes endothelial cells rat′s aortic rings |
| url | http://www.ijpmonline.org/article.asp?issn=0377-4929;year=2012;volume=55;issue=2;spage=211;epage=214;aulast=Mirmiranpour |
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