Enhancing Enzymatic Hydrolysis of Cellulose by Ultrasonic Pretreatment
Slurries of rice-straw cellulose (obtained by delignification and removal of hemicelluloses from the powdered raw material) were subjected to ultrasonic waves at different intensities for various times (constant temperature). Susceptibility of the samples to cellulose-hydrolysis increased initially...
| Main Authors: | , |
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| Format: | Article |
| Language: | English |
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Iranian Institute of Research and Development in Chemical Industries (IRDCI)-ACECR
1998-06-01
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| Series: | Iranian Journal of Chemistry & Chemical Engineering |
| Subjects: | |
| Online Access: | http://www.ijcce.ac.ir/article_10307_7c84bd04b8dc1ad6fec887bddef5015d.pdf |
| _version_ | 1818530710789554176 |
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| author | Roya Mahinpour Mohammad Nabi Sarboluki |
| author_facet | Roya Mahinpour Mohammad Nabi Sarboluki |
| author_sort | Roya Mahinpour |
| collection | DOAJ |
| description | Slurries of rice-straw cellulose (obtained by delignification and removal of hemicelluloses from the powdered raw material) were subjected to ultrasonic waves at different intensities for various times (constant temperature). Susceptibility of the samples to cellulose-hydrolysis increased initially with pretreatment time, reaching a maximum or a constant level thereafter. Maximum glucose yield (~21%) was obtained under the following conditions: enzyme/substrate ratio 0.1; amplitude 18mm (peak to peak), and exposure time 30 minutes. This yield is 30X that of the starting raw material, 10X that of the untreated rice-straw cellulose and 12-13X that of the value reported by others regarding pretreatment of wheat-straw cellulose by phenol. Changing the enzyme/substrate ratio to 0.4 raises glucose yield to 42% under the same conditions. Removal of temperature control during pretreatment (50-80 °C) increases the yield up to ~30% without increasing the enzyme/substrate ratio. |
| first_indexed | 2024-12-11T17:23:10Z |
| format | Article |
| id | doaj.art-2127dba378b74ed9af03fef48de0c078 |
| institution | Directory Open Access Journal |
| issn | 1021-9986 1021-9986 |
| language | English |
| last_indexed | 2024-12-11T17:23:10Z |
| publishDate | 1998-06-01 |
| publisher | Iranian Institute of Research and Development in Chemical Industries (IRDCI)-ACECR |
| record_format | Article |
| series | Iranian Journal of Chemistry & Chemical Engineering |
| spelling | doaj.art-2127dba378b74ed9af03fef48de0c0782022-12-22T00:57:03ZengIranian Institute of Research and Development in Chemical Industries (IRDCI)-ACECRIranian Journal of Chemistry & Chemical Engineering1021-99861021-99861998-06-0117181310307Enhancing Enzymatic Hydrolysis of Cellulose by Ultrasonic PretreatmentRoya Mahinpour0Mohammad Nabi Sarboluki1Department of Chemistry, Kashan University, Zip code 87304, Kashan, I.R. IRANInstitute of Biochemistry and Biophysics, Tehran University, P.O. Box 13145-1384, Tehran, I.R. IRANSlurries of rice-straw cellulose (obtained by delignification and removal of hemicelluloses from the powdered raw material) were subjected to ultrasonic waves at different intensities for various times (constant temperature). Susceptibility of the samples to cellulose-hydrolysis increased initially with pretreatment time, reaching a maximum or a constant level thereafter. Maximum glucose yield (~21%) was obtained under the following conditions: enzyme/substrate ratio 0.1; amplitude 18mm (peak to peak), and exposure time 30 minutes. This yield is 30X that of the starting raw material, 10X that of the untreated rice-straw cellulose and 12-13X that of the value reported by others regarding pretreatment of wheat-straw cellulose by phenol. Changing the enzyme/substrate ratio to 0.4 raises glucose yield to 42% under the same conditions. Removal of temperature control during pretreatment (50-80 °C) increases the yield up to ~30% without increasing the enzyme/substrate ratio.http://www.ijcce.ac.ir/article_10307_7c84bd04b8dc1ad6fec887bddef5015d.pdfcellulosehydrolysiscellulaseenzymeultrasonicphysico-mechanicalpretreatmentrice-straw |
| spellingShingle | Roya Mahinpour Mohammad Nabi Sarboluki Enhancing Enzymatic Hydrolysis of Cellulose by Ultrasonic Pretreatment Iranian Journal of Chemistry & Chemical Engineering cellulose hydrolysis cellulase enzyme ultrasonic physico-mechanical pretreatment rice-straw |
| title | Enhancing Enzymatic Hydrolysis of Cellulose by Ultrasonic Pretreatment |
| title_full | Enhancing Enzymatic Hydrolysis of Cellulose by Ultrasonic Pretreatment |
| title_fullStr | Enhancing Enzymatic Hydrolysis of Cellulose by Ultrasonic Pretreatment |
| title_full_unstemmed | Enhancing Enzymatic Hydrolysis of Cellulose by Ultrasonic Pretreatment |
| title_short | Enhancing Enzymatic Hydrolysis of Cellulose by Ultrasonic Pretreatment |
| title_sort | enhancing enzymatic hydrolysis of cellulose by ultrasonic pretreatment |
| topic | cellulose hydrolysis cellulase enzyme ultrasonic physico-mechanical pretreatment rice-straw |
| url | http://www.ijcce.ac.ir/article_10307_7c84bd04b8dc1ad6fec887bddef5015d.pdf |
| work_keys_str_mv | AT royamahinpour enhancingenzymatichydrolysisofcellulosebyultrasonicpretreatment AT mohammadnabisarboluki enhancingenzymatichydrolysisofcellulosebyultrasonicpretreatment |