AsHSP26.8a, a creeping bentgrass small heat shock protein integrates different signaling pathways to modulate plant abiotic stress response
Abstract Background Small heat shock proteins (sHSPs) are critical for plant response to biotic and abiotic stresses, especially heat stress. They have also been implicated in various aspects of plant development. However, the acting mechanisms of the sHSPs in plants, especially in perennial grass s...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
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BMC
2020-04-01
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| Series: | BMC Plant Biology |
| Subjects: | |
| Online Access: | http://link.springer.com/article/10.1186/s12870-020-02369-5 |
| _version_ | 1819038986697441280 |
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| author | Xinbo Sun Junfei Zhu Xin Li Zhigang Li Liebao Han Hong Luo |
| author_facet | Xinbo Sun Junfei Zhu Xin Li Zhigang Li Liebao Han Hong Luo |
| author_sort | Xinbo Sun |
| collection | DOAJ |
| description | Abstract Background Small heat shock proteins (sHSPs) are critical for plant response to biotic and abiotic stresses, especially heat stress. They have also been implicated in various aspects of plant development. However, the acting mechanisms of the sHSPs in plants, especially in perennial grass species, remain largely elusive. Results In this study, AsHSP26.8a, a novel chloroplast-localized sHSP gene from creeping bentgrass (Agrostis stolonifera L.) was cloned and its role in plant response to environmental stress was studied. AsHSP26.8a encodes a protein of 26.8 kDa. Its expression was strongly induced in both leaf and root tissues by heat stress. Transgenic Arabidopsis plants overexpressing AsHSP26.8a displayed reduced tolerance to heat stress. Furthermore, overexpression of AsHSP26.8a resulted in hypersensitivity to hormone ABA and salinity stress. Global gene expression analysis revealed AsHSP26.8a-modulated expression of heat-shock transcription factor gene, and the involvement of AsHSP26.8a in ABA-dependent and -independent as well as other stress signaling pathways. Conclusions Our results suggest that AsHSP26.8a may negatively regulate plant response to various abiotic stresses through modulating ABA and other stress signaling pathways. |
| first_indexed | 2024-12-21T08:46:02Z |
| format | Article |
| id | doaj.art-212da1cc1dbd4338a70f99b5baf66e58 |
| institution | Directory Open Access Journal |
| issn | 1471-2229 |
| language | English |
| last_indexed | 2024-12-21T08:46:02Z |
| publishDate | 2020-04-01 |
| publisher | BMC |
| record_format | Article |
| series | BMC Plant Biology |
| spelling | doaj.art-212da1cc1dbd4338a70f99b5baf66e582022-12-21T19:09:50ZengBMCBMC Plant Biology1471-22292020-04-0120111910.1186/s12870-020-02369-5AsHSP26.8a, a creeping bentgrass small heat shock protein integrates different signaling pathways to modulate plant abiotic stress responseXinbo Sun0Junfei Zhu1Xin Li2Zhigang Li3Liebao Han4Hong Luo5Key Laboratory of Crop Growth Regulation of Hebei Province, College of Agronomy, Hebei Agricultural UniversityKey Laboratory of Crop Growth Regulation of Hebei Province, College of Agronomy, Hebei Agricultural UniversityKey Laboratory of Crop Growth Regulation of Hebei Province, College of Agronomy, Hebei Agricultural UniversityDepartment of Genetics and Biochemistry, Clemson UniversityTurfgrass Research Institute, Beijing Forestry UniversityDepartment of Genetics and Biochemistry, Clemson UniversityAbstract Background Small heat shock proteins (sHSPs) are critical for plant response to biotic and abiotic stresses, especially heat stress. They have also been implicated in various aspects of plant development. However, the acting mechanisms of the sHSPs in plants, especially in perennial grass species, remain largely elusive. Results In this study, AsHSP26.8a, a novel chloroplast-localized sHSP gene from creeping bentgrass (Agrostis stolonifera L.) was cloned and its role in plant response to environmental stress was studied. AsHSP26.8a encodes a protein of 26.8 kDa. Its expression was strongly induced in both leaf and root tissues by heat stress. Transgenic Arabidopsis plants overexpressing AsHSP26.8a displayed reduced tolerance to heat stress. Furthermore, overexpression of AsHSP26.8a resulted in hypersensitivity to hormone ABA and salinity stress. Global gene expression analysis revealed AsHSP26.8a-modulated expression of heat-shock transcription factor gene, and the involvement of AsHSP26.8a in ABA-dependent and -independent as well as other stress signaling pathways. Conclusions Our results suggest that AsHSP26.8a may negatively regulate plant response to various abiotic stresses through modulating ABA and other stress signaling pathways.http://link.springer.com/article/10.1186/s12870-020-02369-5Creeping bentgrassAsHSP26.8aABAAbiotic stressSmall heat shock protein |
| spellingShingle | Xinbo Sun Junfei Zhu Xin Li Zhigang Li Liebao Han Hong Luo AsHSP26.8a, a creeping bentgrass small heat shock protein integrates different signaling pathways to modulate plant abiotic stress response BMC Plant Biology Creeping bentgrass AsHSP26.8a ABA Abiotic stress Small heat shock protein |
| title | AsHSP26.8a, a creeping bentgrass small heat shock protein integrates different signaling pathways to modulate plant abiotic stress response |
| title_full | AsHSP26.8a, a creeping bentgrass small heat shock protein integrates different signaling pathways to modulate plant abiotic stress response |
| title_fullStr | AsHSP26.8a, a creeping bentgrass small heat shock protein integrates different signaling pathways to modulate plant abiotic stress response |
| title_full_unstemmed | AsHSP26.8a, a creeping bentgrass small heat shock protein integrates different signaling pathways to modulate plant abiotic stress response |
| title_short | AsHSP26.8a, a creeping bentgrass small heat shock protein integrates different signaling pathways to modulate plant abiotic stress response |
| title_sort | ashsp26 8a a creeping bentgrass small heat shock protein integrates different signaling pathways to modulate plant abiotic stress response |
| topic | Creeping bentgrass AsHSP26.8a ABA Abiotic stress Small heat shock protein |
| url | http://link.springer.com/article/10.1186/s12870-020-02369-5 |
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