Reconstruction and Analysis of the Immune-Related LINC00987/A2M Axis in Lung Adenocarcinoma
Enhancer RNAs (eRNAs) participate in tumor growth and immune regulation through complex signaling pathways. However, the immune-related function of the eRNA-mRNA axis in lung adenocarcinoma (LUAD) is unclear. Data on the expression of eRNAs and mRNAs were downloaded from The Cancer Genome Atlas, GEO...
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Frontiers Media S.A.
2021-04-01
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| Series: | Frontiers in Molecular Biosciences |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fmolb.2021.644557/full |
| _version_ | 1818726961704337408 |
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| author | Jiakang Ma Xiaoyan Lin Xueting Wang Qingqing Min Tonglian Wang Chaozhi Tang |
| author_facet | Jiakang Ma Xiaoyan Lin Xueting Wang Qingqing Min Tonglian Wang Chaozhi Tang |
| author_sort | Jiakang Ma |
| collection | DOAJ |
| description | Enhancer RNAs (eRNAs) participate in tumor growth and immune regulation through complex signaling pathways. However, the immune-related function of the eRNA-mRNA axis in lung adenocarcinoma (LUAD) is unclear. Data on the expression of eRNAs and mRNAs were downloaded from The Cancer Genome Atlas, GEO, and UCSC Xena, including LUAD, and pan-cancer clinical data and mutational information. Immune gene files were obtained from ImmLnc and ImmPort databases. Survival indices, including relapse-free and overall survival, were analyzed using the Kaplan–Meier and log-rank methods. The level of immune cell infiltration, degree of tumor hypoxia, and tumor cell stemness characteristics were quantified using the single-sample gene set enrichment analysis algorithm. The immune infiltration score and infiltration degree were evaluated using the ESTIMATE and CIBERSORT algorithms. The tumor mutation burden and microsatellite instability were examined using the Spearman test. The LUAD-associated immune-related LINC00987/A2M axis was down-regulated in most cancer types, indicating poor survival and cancer progression. Immune cell infiltration was closely related to abnormal expression of the LINC00987/A2M axis, linking its expression to a possible evaluation of sensitivity to checkpoint inhibitors and response to chemotherapy. Abnormal expression of the LINC00987/A2M axis was characterized by heterogeneity in the degree of tumor hypoxia and stemness characteristics. The abnormal distribution of immune cells in LUAD was also verified through pan-cancer analysis. Comprehensive bioinformatic analysis showed that the LINC00987/A2M axis is a functional and effective tumor suppressor and biomarker for assessing the immune microenvironment and prognostic and therapeutic evaluations of LUAD. |
| first_indexed | 2024-12-17T22:06:31Z |
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| id | doaj.art-212e32584f3f457e99bb36b52e28acaa |
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| language | English |
| last_indexed | 2024-12-17T22:06:31Z |
| publishDate | 2021-04-01 |
| publisher | Frontiers Media S.A. |
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| series | Frontiers in Molecular Biosciences |
| spelling | doaj.art-212e32584f3f457e99bb36b52e28acaa2022-12-21T21:30:51ZengFrontiers Media S.A.Frontiers in Molecular Biosciences2296-889X2021-04-01810.3389/fmolb.2021.644557644557Reconstruction and Analysis of the Immune-Related LINC00987/A2M Axis in Lung AdenocarcinomaJiakang Ma0Xiaoyan Lin1Xueting Wang2Qingqing Min3Tonglian Wang4Chaozhi Tang5Department of Oncology, The Second Affiliated Hospital of Zhengzhou University, Zhengzhou, ChinaDepartment of Oncology, The Second Affiliated Hospital of Zhengzhou University, Zhengzhou, ChinaDepartment of Ophthalmology, The First Affiliated Hospital and College of Clinical Medicine of Henan University of Science and Technology, Luoyang, ChinaDepartment of Endodontics, Stomatological Hospital of China Medical University, Shenyang, ChinaResearch Center of Molecular Medicine of Yunnan Province, Faculty of Life Science and Technology, Kunming University of Science and Technology, Kunming, ChinaDepartment of Urology, The First Affiliated Hospital of China Medical University, Shenyang, ChinaEnhancer RNAs (eRNAs) participate in tumor growth and immune regulation through complex signaling pathways. However, the immune-related function of the eRNA-mRNA axis in lung adenocarcinoma (LUAD) is unclear. Data on the expression of eRNAs and mRNAs were downloaded from The Cancer Genome Atlas, GEO, and UCSC Xena, including LUAD, and pan-cancer clinical data and mutational information. Immune gene files were obtained from ImmLnc and ImmPort databases. Survival indices, including relapse-free and overall survival, were analyzed using the Kaplan–Meier and log-rank methods. The level of immune cell infiltration, degree of tumor hypoxia, and tumor cell stemness characteristics were quantified using the single-sample gene set enrichment analysis algorithm. The immune infiltration score and infiltration degree were evaluated using the ESTIMATE and CIBERSORT algorithms. The tumor mutation burden and microsatellite instability were examined using the Spearman test. The LUAD-associated immune-related LINC00987/A2M axis was down-regulated in most cancer types, indicating poor survival and cancer progression. Immune cell infiltration was closely related to abnormal expression of the LINC00987/A2M axis, linking its expression to a possible evaluation of sensitivity to checkpoint inhibitors and response to chemotherapy. Abnormal expression of the LINC00987/A2M axis was characterized by heterogeneity in the degree of tumor hypoxia and stemness characteristics. The abnormal distribution of immune cells in LUAD was also verified through pan-cancer analysis. Comprehensive bioinformatic analysis showed that the LINC00987/A2M axis is a functional and effective tumor suppressor and biomarker for assessing the immune microenvironment and prognostic and therapeutic evaluations of LUAD.https://www.frontiersin.org/articles/10.3389/fmolb.2021.644557/fulleRNALUADLINC00987/A2M axisimmune cell infiltrationtumor hypoxiatumor cell stemness |
| spellingShingle | Jiakang Ma Xiaoyan Lin Xueting Wang Qingqing Min Tonglian Wang Chaozhi Tang Reconstruction and Analysis of the Immune-Related LINC00987/A2M Axis in Lung Adenocarcinoma Frontiers in Molecular Biosciences eRNA LUAD LINC00987/A2M axis immune cell infiltration tumor hypoxia tumor cell stemness |
| title | Reconstruction and Analysis of the Immune-Related LINC00987/A2M Axis in Lung Adenocarcinoma |
| title_full | Reconstruction and Analysis of the Immune-Related LINC00987/A2M Axis in Lung Adenocarcinoma |
| title_fullStr | Reconstruction and Analysis of the Immune-Related LINC00987/A2M Axis in Lung Adenocarcinoma |
| title_full_unstemmed | Reconstruction and Analysis of the Immune-Related LINC00987/A2M Axis in Lung Adenocarcinoma |
| title_short | Reconstruction and Analysis of the Immune-Related LINC00987/A2M Axis in Lung Adenocarcinoma |
| title_sort | reconstruction and analysis of the immune related linc00987 a2m axis in lung adenocarcinoma |
| topic | eRNA LUAD LINC00987/A2M axis immune cell infiltration tumor hypoxia tumor cell stemness |
| url | https://www.frontiersin.org/articles/10.3389/fmolb.2021.644557/full |
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