passive immunization with Candida albicans antiproteinase as a prophylactic tool against the candidiasis
Summary: Background: life threating fungel disease is now a frequent substantial of the immunocomprimised host population . Candida infection has raised throughout the era of antibiotics & immunosuppressive chemotherapy. Aim of study : to highlight the need for alternative immunprophlactic tool...
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| Format: | Article |
| Language: | English |
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College of Medicine University of Baghdad
2005-07-01
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| Series: | مجلة كلية الطب |
| Subjects: | |
| Online Access: | http://iqjmc.uobaghdad.edu.iq/index.php/19JFacMedBaghdad36/article/view/1651 |
| _version_ | 1797332995196059648 |
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| author | Azhar A.I. Drshim rassool A Badr sand E. Badr |
| author_facet | Azhar A.I. Drshim rassool A Badr sand E. Badr |
| author_sort | Azhar A.I. Drshim |
| collection | DOAJ |
| description | Summary:
Background: life threating fungel disease is now a frequent substantial of the immunocomprimised host population . Candida infection has raised throughout the era of antibiotics & immunosuppressive chemotherapy.
Aim of study : to highlight the need for alternative immunprophlactic tool .Therefore the need to develop prophylactic or therapeutic immunolglobulin against candidacies Materials & Methods: 12.5 mg/ml. of Candida albicans proteinase enzyme was injected S/C into a group of five rabbits weighing 3 Kg. with complete Freund's adjuvant. Antisera was collected from the animals after 28 days . 0.2 ml of this antisera given l/V into two group of rabbits ; followed by 0.1 ml of the antiserum one of thses groups as control. the 21"1 group challenged with 2.8*10^7 cfu/ml of actively growing C. abicans for three days . the 3rd group received only 2,8*10A7 cfu/ml ofC. albicans for three days
Results: Test rabbits , which received both antisera and C. albicans , were completely protected when examined after six weeks . No symptoms of the diaease ; no pathological lesions could be seen in the organs ( Kidneys, liver and spleen ). Culture for C. albicans from these organs were all negative . In contrast to rabbits received challenged dose of Candida; they become sick , visceral organs showed profuse microabscesses . Candida was isolated from minced organs on culture .
Conclusion : the findings draws attention to an important fact that the passive immunizention can be applied to protect patients with leukemia and others immunocompromised individuals from serious infection of Candida . This discovery calles for application to open anew hope for these patients at least to diverse their suffer for sometime, |
| first_indexed | 2024-03-08T07:57:26Z |
| format | Article |
| id | doaj.art-212e4353cbb1426397d864ec9814bb55 |
| institution | Directory Open Access Journal |
| issn | 0041-9419 2410-8057 |
| language | English |
| last_indexed | 2024-03-08T07:57:26Z |
| publishDate | 2005-07-01 |
| publisher | College of Medicine University of Baghdad |
| record_format | Article |
| series | مجلة كلية الطب |
| spelling | doaj.art-212e4353cbb1426397d864ec9814bb552024-02-02T13:23:33ZengCollege of Medicine University of Baghdadمجلة كلية الطب0041-94192410-80572005-07-0147210.32007/1651159-161passive immunization with Candida albicans antiproteinase as a prophylactic tool against the candidiasisAzhar A.I. Drshim0rassool A Badr1sand E. Badr2Department of microbiology, college of medicine, Baghdad university, Iraq.Dept. of obstetric & Gynecology, college of medicine, Baghdad university, Iraq.Department of histopathology, college of Dentistry, Baghdad university, Iraq.Summary: Background: life threating fungel disease is now a frequent substantial of the immunocomprimised host population . Candida infection has raised throughout the era of antibiotics & immunosuppressive chemotherapy. Aim of study : to highlight the need for alternative immunprophlactic tool .Therefore the need to develop prophylactic or therapeutic immunolglobulin against candidacies Materials & Methods: 12.5 mg/ml. of Candida albicans proteinase enzyme was injected S/C into a group of five rabbits weighing 3 Kg. with complete Freund's adjuvant. Antisera was collected from the animals after 28 days . 0.2 ml of this antisera given l/V into two group of rabbits ; followed by 0.1 ml of the antiserum one of thses groups as control. the 21"1 group challenged with 2.8*10^7 cfu/ml of actively growing C. abicans for three days . the 3rd group received only 2,8*10A7 cfu/ml ofC. albicans for three days Results: Test rabbits , which received both antisera and C. albicans , were completely protected when examined after six weeks . No symptoms of the diaease ; no pathological lesions could be seen in the organs ( Kidneys, liver and spleen ). Culture for C. albicans from these organs were all negative . In contrast to rabbits received challenged dose of Candida; they become sick , visceral organs showed profuse microabscesses . Candida was isolated from minced organs on culture . Conclusion : the findings draws attention to an important fact that the passive immunizention can be applied to protect patients with leukemia and others immunocompromised individuals from serious infection of Candida . This discovery calles for application to open anew hope for these patients at least to diverse their suffer for sometime,http://iqjmc.uobaghdad.edu.iq/index.php/19JFacMedBaghdad36/article/view/1651sometime, immunolglobulin, mtnunocomprmised |
| spellingShingle | Azhar A.I. Drshim rassool A Badr sand E. Badr passive immunization with Candida albicans antiproteinase as a prophylactic tool against the candidiasis مجلة كلية الطب sometime, immunolglobulin, mtnunocomprmised |
| title | passive immunization with Candida albicans antiproteinase as a prophylactic tool against the candidiasis |
| title_full | passive immunization with Candida albicans antiproteinase as a prophylactic tool against the candidiasis |
| title_fullStr | passive immunization with Candida albicans antiproteinase as a prophylactic tool against the candidiasis |
| title_full_unstemmed | passive immunization with Candida albicans antiproteinase as a prophylactic tool against the candidiasis |
| title_short | passive immunization with Candida albicans antiproteinase as a prophylactic tool against the candidiasis |
| title_sort | passive immunization with candida albicans antiproteinase as a prophylactic tool against the candidiasis |
| topic | sometime, immunolglobulin, mtnunocomprmised |
| url | http://iqjmc.uobaghdad.edu.iq/index.php/19JFacMedBaghdad36/article/view/1651 |
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