Ultra-sensitive real-time detection of cancer-derived exosomes directly from cell supernatants by a large Goos–Hänchen signal generation on plasmonic sensing interface
Exosomes have shown great potential in serving as a cancer biomarker over these years since they carry crucial information of their parent cells. Therefore, detection of exosomes is of vital importance to the early-stage diagnostics of multiple major diseases. In this paper, we have proposed a real-...
| Main Authors: | , , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Elsevier
2023-12-01
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| Series: | Biosensors and Bioelectronics: X |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S2590137023000882 |
| _version_ | 1797398474161913856 |
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| author | Manel Hedhly Yuye Wang Aude Brunel Flavien Beffara Hussein Akil Mireille Verdier Barbara Bessette Aurelian Crunteanu Ho-Pui Ho Georges Humbert Fabrice Lalloue Shuwen Zeng |
| author_facet | Manel Hedhly Yuye Wang Aude Brunel Flavien Beffara Hussein Akil Mireille Verdier Barbara Bessette Aurelian Crunteanu Ho-Pui Ho Georges Humbert Fabrice Lalloue Shuwen Zeng |
| author_sort | Manel Hedhly |
| collection | DOAJ |
| description | Exosomes have shown great potential in serving as a cancer biomarker over these years since they carry crucial information of their parent cells. Therefore, detection of exosomes is of vital importance to the early-stage diagnostics of multiple major diseases. In this paper, we have proposed a real-time and label-free sensing technique based on 2D Ge2Sb2Te5 (GST) nanomaterial enhanced plasmonic substrate. We have achieved a detection limit down to 104 exosomes/mL based on Goos–Hänchen (GH) shift measurement, which is more than two orders of magnitude superior to conventional SPR sensing techniques. Moreover, the detection of unpurified exosomes directly in cell culture supernatant (CCS) has been successfully demonstrated with a significant experimental GH shift signal of more than 120 μm been detected. Multiple control experiments using exosome-free solutions and control antibody coated substrates have also been performed, which validates the specificity of our device. The proposed plasmonic sensing scheme with enhanced sensing performance has the capability of detecting exosomes at low concentration levels. It also possesses the ability of direct detection of exosomes in CCS, which offers a convenient and efficient platform for exosome detection and analysis. We envision that this technique can serve as a promising tool in early-stage clinical diagnostics and treatment. |
| first_indexed | 2024-03-09T01:26:05Z |
| format | Article |
| id | doaj.art-212e63aced6d45e785b18bd6a5289a65 |
| institution | Directory Open Access Journal |
| issn | 2590-1370 |
| language | English |
| last_indexed | 2024-03-09T01:26:05Z |
| publishDate | 2023-12-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Biosensors and Bioelectronics: X |
| spelling | doaj.art-212e63aced6d45e785b18bd6a5289a652023-12-10T06:17:24ZengElsevierBiosensors and Bioelectronics: X2590-13702023-12-0115100391Ultra-sensitive real-time detection of cancer-derived exosomes directly from cell supernatants by a large Goos–Hänchen signal generation on plasmonic sensing interfaceManel Hedhly0Yuye Wang1Aude Brunel2Flavien Beffara3Hussein Akil4Mireille Verdier5Barbara Bessette6Aurelian Crunteanu7Ho-Pui Ho8Georges Humbert9Fabrice Lalloue10Shuwen Zeng11XLIM Research Institute, UMR 7252 CNRS/University of Limoges, 123 Avenue Albert Thomas, 87060, Limoges, France; Faculty of Sciences of Tunis, Université de Tunis El Manar, 2092-El Manar, Tunis, 1068, TunisiaXLIM Research Institute, UMR 7252 CNRS/University of Limoges, 123 Avenue Albert Thomas, 87060, Limoges, France; Department of Biomedical Engineering, The Chinese University of Hong Kong, Shatin, N.T.,Hong Kong, 999077, China; Bionic Sensing and Intelligence Center, Institute of Biomedical and Health Engineering, Shenzhen Institute of Advanced Technology, Chinese Academy of Sciences, Shenzhen, 518055, ChinaUMR Inserm 1308, CAPTuR, Facuty of Medicine, University of Limoges, 2 rue du Dr Marcland 87025, Limoges, FranceXLIM Research Institute, UMR 7252 CNRS/University of Limoges, 123 Avenue Albert Thomas, 87060, Limoges, FranceUMR Inserm 1308, CAPTuR, Facuty of Medicine, University of Limoges, 2 rue du Dr Marcland 87025, Limoges, FranceUMR Inserm 1308, CAPTuR, Facuty of Medicine, University of Limoges, 2 rue du Dr Marcland 87025, Limoges, FranceUMR Inserm 1308, CAPTuR, Facuty of Medicine, University of Limoges, 2 rue du Dr Marcland 87025, Limoges, FranceXLIM Research Institute, UMR 7252 CNRS/University of Limoges, 123 Avenue Albert Thomas, 87060, Limoges, FranceDepartment of Biomedical Engineering, The Chinese University of Hong Kong, Shatin, N.T.,Hong Kong, 999077, ChinaXLIM Research Institute, UMR 7252 CNRS/University of Limoges, 123 Avenue Albert Thomas, 87060, Limoges, France; Corresponding author.UMR Inserm 1308, CAPTuR, Facuty of Medicine, University of Limoges, 2 rue du Dr Marcland 87025, Limoges, France; Corresponding author.XLIM Research Institute, UMR 7252 CNRS/University of Limoges, 123 Avenue Albert Thomas, 87060, Limoges, France; Light, Nanomaterials & Nanotechnologies (L2n), CNRS-EMR 7004, Université de Technologie de Troyes, 10000, Troyes, France; Corresponding author. XLIM Research Institute, UMR 7252 CNRS/University of Limoges, 123 Avenue Albert Thomas, 87060, Limoges, France.Exosomes have shown great potential in serving as a cancer biomarker over these years since they carry crucial information of their parent cells. Therefore, detection of exosomes is of vital importance to the early-stage diagnostics of multiple major diseases. In this paper, we have proposed a real-time and label-free sensing technique based on 2D Ge2Sb2Te5 (GST) nanomaterial enhanced plasmonic substrate. We have achieved a detection limit down to 104 exosomes/mL based on Goos–Hänchen (GH) shift measurement, which is more than two orders of magnitude superior to conventional SPR sensing techniques. Moreover, the detection of unpurified exosomes directly in cell culture supernatant (CCS) has been successfully demonstrated with a significant experimental GH shift signal of more than 120 μm been detected. Multiple control experiments using exosome-free solutions and control antibody coated substrates have also been performed, which validates the specificity of our device. The proposed plasmonic sensing scheme with enhanced sensing performance has the capability of detecting exosomes at low concentration levels. It also possesses the ability of direct detection of exosomes in CCS, which offers a convenient and efficient platform for exosome detection and analysis. We envision that this technique can serve as a promising tool in early-stage clinical diagnostics and treatment.http://www.sciencedirect.com/science/article/pii/S2590137023000882PlasmonicsPhase singularityGoos–hänchen shiftExosomesDirect detection |
| spellingShingle | Manel Hedhly Yuye Wang Aude Brunel Flavien Beffara Hussein Akil Mireille Verdier Barbara Bessette Aurelian Crunteanu Ho-Pui Ho Georges Humbert Fabrice Lalloue Shuwen Zeng Ultra-sensitive real-time detection of cancer-derived exosomes directly from cell supernatants by a large Goos–Hänchen signal generation on plasmonic sensing interface Biosensors and Bioelectronics: X Plasmonics Phase singularity Goos–hänchen shift Exosomes Direct detection |
| title | Ultra-sensitive real-time detection of cancer-derived exosomes directly from cell supernatants by a large Goos–Hänchen signal generation on plasmonic sensing interface |
| title_full | Ultra-sensitive real-time detection of cancer-derived exosomes directly from cell supernatants by a large Goos–Hänchen signal generation on plasmonic sensing interface |
| title_fullStr | Ultra-sensitive real-time detection of cancer-derived exosomes directly from cell supernatants by a large Goos–Hänchen signal generation on plasmonic sensing interface |
| title_full_unstemmed | Ultra-sensitive real-time detection of cancer-derived exosomes directly from cell supernatants by a large Goos–Hänchen signal generation on plasmonic sensing interface |
| title_short | Ultra-sensitive real-time detection of cancer-derived exosomes directly from cell supernatants by a large Goos–Hänchen signal generation on plasmonic sensing interface |
| title_sort | ultra sensitive real time detection of cancer derived exosomes directly from cell supernatants by a large goos hanchen signal generation on plasmonic sensing interface |
| topic | Plasmonics Phase singularity Goos–hänchen shift Exosomes Direct detection |
| url | http://www.sciencedirect.com/science/article/pii/S2590137023000882 |
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