MicroRNA 10a marks regulatory T cells.
MicroRNAs (miRNAs) are crucial for regulatory T cell (Treg) stability and function. We report that microRNA-10a (miR-10a) is expressed in Tregs but not in other T cells including individual thymocyte subsets. Expression profiling in inbred mouse strains demonstrated that non-obese diabetic (NOD) mic...
| Main Authors: | , , , , , , , |
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| Format: | Article |
| Language: | English |
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Public Library of Science (PLoS)
2012-01-01
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| Series: | PLoS ONE |
| Online Access: | http://europepmc.org/articles/PMC3356350?pdf=render |
| _version_ | 1818525075599523840 |
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| author | Lukas T Jeker Xuyu Zhou Kseniya Gershberg Dimitri de Kouchkovsky Malika M Morar Gustavo Stadthagen Anders H Lund Jeffrey A Bluestone |
| author_facet | Lukas T Jeker Xuyu Zhou Kseniya Gershberg Dimitri de Kouchkovsky Malika M Morar Gustavo Stadthagen Anders H Lund Jeffrey A Bluestone |
| author_sort | Lukas T Jeker |
| collection | DOAJ |
| description | MicroRNAs (miRNAs) are crucial for regulatory T cell (Treg) stability and function. We report that microRNA-10a (miR-10a) is expressed in Tregs but not in other T cells including individual thymocyte subsets. Expression profiling in inbred mouse strains demonstrated that non-obese diabetic (NOD) mice with a genetic susceptibility for autoimmune diabetes have lower Treg-specific miR-10a expression than C57BL/6J autoimmune resistant mice. Inhibition of miR-10a expression in vitro leads to reduced FoxP3 expression levels and miR-10a expression is lower in unstable "exFoxP3" T cells. Unstable in vitro TGF-ß-induced, iTregs do not express miR-10a unless cultured in the presence of retinoic acid (RA) which has been associated with increased stability of iTreg, suggesting that miR-10a might play a role in stabilizing Treg. However, genetic ablation of miR-10a neither affected the number and phenotype of natural Treg nor the capacity of conventional T cells to induce FoxP3 in response to TGFβ, RA, or a combination of the two. Thus, miR-10a is selectively expressed in Treg but inhibition by antagomiRs or genetic ablation resulted in discordant effects on FoxP3. |
| first_indexed | 2024-12-11T06:04:54Z |
| format | Article |
| id | doaj.art-2130618c004c4436be4393e71590b5da |
| institution | Directory Open Access Journal |
| issn | 1932-6203 |
| language | English |
| last_indexed | 2024-12-11T06:04:54Z |
| publishDate | 2012-01-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS ONE |
| spelling | doaj.art-2130618c004c4436be4393e71590b5da2022-12-22T01:18:20ZengPublic Library of Science (PLoS)PLoS ONE1932-62032012-01-0175e3668410.1371/journal.pone.0036684MicroRNA 10a marks regulatory T cells.Lukas T JekerXuyu ZhouKseniya GershbergDimitri de KouchkovskyMalika M MorarGustavo StadthagenAnders H LundJeffrey A BluestoneMicroRNAs (miRNAs) are crucial for regulatory T cell (Treg) stability and function. We report that microRNA-10a (miR-10a) is expressed in Tregs but not in other T cells including individual thymocyte subsets. Expression profiling in inbred mouse strains demonstrated that non-obese diabetic (NOD) mice with a genetic susceptibility for autoimmune diabetes have lower Treg-specific miR-10a expression than C57BL/6J autoimmune resistant mice. Inhibition of miR-10a expression in vitro leads to reduced FoxP3 expression levels and miR-10a expression is lower in unstable "exFoxP3" T cells. Unstable in vitro TGF-ß-induced, iTregs do not express miR-10a unless cultured in the presence of retinoic acid (RA) which has been associated with increased stability of iTreg, suggesting that miR-10a might play a role in stabilizing Treg. However, genetic ablation of miR-10a neither affected the number and phenotype of natural Treg nor the capacity of conventional T cells to induce FoxP3 in response to TGFβ, RA, or a combination of the two. Thus, miR-10a is selectively expressed in Treg but inhibition by antagomiRs or genetic ablation resulted in discordant effects on FoxP3.http://europepmc.org/articles/PMC3356350?pdf=render |
| spellingShingle | Lukas T Jeker Xuyu Zhou Kseniya Gershberg Dimitri de Kouchkovsky Malika M Morar Gustavo Stadthagen Anders H Lund Jeffrey A Bluestone MicroRNA 10a marks regulatory T cells. PLoS ONE |
| title | MicroRNA 10a marks regulatory T cells. |
| title_full | MicroRNA 10a marks regulatory T cells. |
| title_fullStr | MicroRNA 10a marks regulatory T cells. |
| title_full_unstemmed | MicroRNA 10a marks regulatory T cells. |
| title_short | MicroRNA 10a marks regulatory T cells. |
| title_sort | microrna 10a marks regulatory t cells |
| url | http://europepmc.org/articles/PMC3356350?pdf=render |
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