Prism Design for Spectral Flow Cytometry
Flow cytometers are instruments used for the rapid quantitative analysis of cell suspension. Traditional flow cytometry uses multi-channel filters to detect fluorescence, whereas full-spectrum fluorescence based on dispersion detection is a more effective and accurate method. The application of vari...
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MDPI AG
2023-01-01
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| Series: | Micromachines |
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| Online Access: | https://www.mdpi.com/2072-666X/14/2/315 |
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| author | Zixi Chao Yong Han Zeheng Jiao Zheng You Jingjing Zhao |
| author_facet | Zixi Chao Yong Han Zeheng Jiao Zheng You Jingjing Zhao |
| author_sort | Zixi Chao |
| collection | DOAJ |
| description | Flow cytometers are instruments used for the rapid quantitative analysis of cell suspension. Traditional flow cytometry uses multi-channel filters to detect fluorescence, whereas full-spectrum fluorescence based on dispersion detection is a more effective and accurate method. The application of various dispersion schemes in flow cytometry spectroscopy has been studied. From the perspective of modern detectors and demand for the miniaturization of flow cytometry, prism dispersion exhibits higher and more uniform light energy utilization, meaning that it is a more suitable dispersion method for small flow cytometers, such as microfluidic flow cytometers. Prism dispersion designs include the size, number, and placement of prisms. By deducing the formula of the final position of light passing through the prism and combining it with the formula of transmittance, the design criteria of the top angle and the incident angle of the prism in pursuit of the optimum transmittance and dispersion index can be obtained. Considering the case of multiple prisms, under the premise of pursuing a smaller size, the optimal design criteria for dispersion system composed of multiple prisms can be obtained. The design of prism dispersion fluorescence detection was demonstrated with a microfluidic flow cytometer, and the effectiveness of the design results was verified by microsphere experiments and practical biological experiments. This design criterion developed in this study is generally applicable to spectral flow cytometers. |
| first_indexed | 2024-03-11T08:23:51Z |
| format | Article |
| id | doaj.art-21348820efc542719523d9907d1727df |
| institution | Directory Open Access Journal |
| issn | 2072-666X |
| language | English |
| last_indexed | 2024-03-11T08:23:51Z |
| publishDate | 2023-01-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Micromachines |
| spelling | doaj.art-21348820efc542719523d9907d1727df2023-11-16T22:10:32ZengMDPI AGMicromachines2072-666X2023-01-0114231510.3390/mi14020315Prism Design for Spectral Flow CytometryZixi Chao0Yong Han1Zeheng Jiao2Zheng You3Jingjing Zhao4Department of Precision Instruments, Tsinghua University, Beijing 100084, ChinaDepartment of Precision Instruments, Tsinghua University, Beijing 100084, ChinaDepartment of Precision Instruments, Tsinghua University, Beijing 100084, ChinaDepartment of Precision Instruments, Tsinghua University, Beijing 100084, ChinaDepartment of Structural Biology, School of Medicine, Stanford University, Stanford, CA 94305, USAFlow cytometers are instruments used for the rapid quantitative analysis of cell suspension. Traditional flow cytometry uses multi-channel filters to detect fluorescence, whereas full-spectrum fluorescence based on dispersion detection is a more effective and accurate method. The application of various dispersion schemes in flow cytometry spectroscopy has been studied. From the perspective of modern detectors and demand for the miniaturization of flow cytometry, prism dispersion exhibits higher and more uniform light energy utilization, meaning that it is a more suitable dispersion method for small flow cytometers, such as microfluidic flow cytometers. Prism dispersion designs include the size, number, and placement of prisms. By deducing the formula of the final position of light passing through the prism and combining it with the formula of transmittance, the design criteria of the top angle and the incident angle of the prism in pursuit of the optimum transmittance and dispersion index can be obtained. Considering the case of multiple prisms, under the premise of pursuing a smaller size, the optimal design criteria for dispersion system composed of multiple prisms can be obtained. The design of prism dispersion fluorescence detection was demonstrated with a microfluidic flow cytometer, and the effectiveness of the design results was verified by microsphere experiments and practical biological experiments. This design criterion developed in this study is generally applicable to spectral flow cytometers.https://www.mdpi.com/2072-666X/14/2/315flow cytometermicro-flow cytometerspectrometryprismdispersion |
| spellingShingle | Zixi Chao Yong Han Zeheng Jiao Zheng You Jingjing Zhao Prism Design for Spectral Flow Cytometry Micromachines flow cytometer micro-flow cytometer spectrometry prism dispersion |
| title | Prism Design for Spectral Flow Cytometry |
| title_full | Prism Design for Spectral Flow Cytometry |
| title_fullStr | Prism Design for Spectral Flow Cytometry |
| title_full_unstemmed | Prism Design for Spectral Flow Cytometry |
| title_short | Prism Design for Spectral Flow Cytometry |
| title_sort | prism design for spectral flow cytometry |
| topic | flow cytometer micro-flow cytometer spectrometry prism dispersion |
| url | https://www.mdpi.com/2072-666X/14/2/315 |
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