A Novel Lepidoptera bioassay analysed using a reduced GUTS model
Lepidopteran species can be both pests and also beneficial pollinators for agricultural crops. However, despite these important roles, the effects of pesticides on this diverse taxa are relatively understudied. To facilitate the assessment of pesticides and other chemical hazards on this taxa, we pr...
| Main Authors: | , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Elsevier
2023-02-01
|
| Series: | Ecotoxicology and Environmental Safety |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S0147651323000088 |
| _version_ | 1828046698002251776 |
|---|---|
| author | Claire Badder Sylvain Bart Alex Robinson Helen Hesketh Peter Kille David J. Spurgeon |
| author_facet | Claire Badder Sylvain Bart Alex Robinson Helen Hesketh Peter Kille David J. Spurgeon |
| author_sort | Claire Badder |
| collection | DOAJ |
| description | Lepidopteran species can be both pests and also beneficial pollinators for agricultural crops. However, despite these important roles, the effects of pesticides on this diverse taxa are relatively understudied. To facilitate the assessment of pesticides and other chemical hazards on this taxa, we present a novel bioassay capable of testing chemical sensitivity to lepidopteran larvae through dietary exposure. We used Mamestra brassicae caterpillars as a model lepidopteran and tested their sensitivity for the organophosphate insecticide chlorpyrifos. We exposed larvae to an artificial diet spiked with chlorpyrifos and monitored survival over time, as well as weight change over a 96-hour exposure period. To test the repeatability and reliability of the developed bioassay, the experiment was repeated three times. The survival in time data collected enabled analysis with the General Unified Threshold of Survival (GUTS) model, recently recognized by EFSA as a ready-to-use tool for regulatory purposes. The GUTS modelling was used to derive a set of relevant toxicokinetic and toxicodynamic parameters relating to the larval response to exposure over time. We found that across the three repeats studies there was no more than a threefold difference in LC₅₀ values (13.1, 18.7 and 8.1 mg/Kg) at 48 h and fourfold difference at 96 h, highlighting the repeatability of the bioassay. We also highlighted the potential of the method to observe sub-lethal effects such as changes in weight. Finally, we discuss the applications of this new bioassay method to chemical risk assessments and its potential for use in other scenarios, such as mixture or pulsed exposure testing. |
| first_indexed | 2024-04-10T18:29:35Z |
| format | Article |
| id | doaj.art-2138c3590ed4408e88a63c8b6d4476e2 |
| institution | Directory Open Access Journal |
| issn | 0147-6513 |
| language | English |
| last_indexed | 2024-04-10T18:29:35Z |
| publishDate | 2023-02-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Ecotoxicology and Environmental Safety |
| spelling | doaj.art-2138c3590ed4408e88a63c8b6d4476e22023-02-02T04:46:51ZengElsevierEcotoxicology and Environmental Safety0147-65132023-02-01251114504A Novel Lepidoptera bioassay analysed using a reduced GUTS modelClaire Badder0Sylvain Bart1Alex Robinson2Helen Hesketh3Peter Kille4David J. Spurgeon5UK Centre for Ecology and Hydrology, MacLean Building, Benson Lane, Oxon, Wallingford OX10 8BB, UKUK Centre for Ecology and Hydrology, MacLean Building, Benson Lane, Oxon, Wallingford OX10 8BB, UK; University of York, Heslington YO10, 5DD, United Kingdom; MO-ECO2 (Modelling and data analyses for ecology and ecotoxicology), Paris, FranceUK Centre for Ecology and Hydrology, MacLean Building, Benson Lane, Oxon, Wallingford OX10 8BB, UKUK Centre for Ecology and Hydrology, MacLean Building, Benson Lane, Oxon, Wallingford OX10 8BB, UKCardiff University, School of Biosciences, Sir Martin Evans Building, The Museum Avenue, CF10 3AX, United KingdomUK Centre for Ecology and Hydrology, MacLean Building, Benson Lane, Oxon, Wallingford OX10 8BB, UK; Corresponding author.Lepidopteran species can be both pests and also beneficial pollinators for agricultural crops. However, despite these important roles, the effects of pesticides on this diverse taxa are relatively understudied. To facilitate the assessment of pesticides and other chemical hazards on this taxa, we present a novel bioassay capable of testing chemical sensitivity to lepidopteran larvae through dietary exposure. We used Mamestra brassicae caterpillars as a model lepidopteran and tested their sensitivity for the organophosphate insecticide chlorpyrifos. We exposed larvae to an artificial diet spiked with chlorpyrifos and monitored survival over time, as well as weight change over a 96-hour exposure period. To test the repeatability and reliability of the developed bioassay, the experiment was repeated three times. The survival in time data collected enabled analysis with the General Unified Threshold of Survival (GUTS) model, recently recognized by EFSA as a ready-to-use tool for regulatory purposes. The GUTS modelling was used to derive a set of relevant toxicokinetic and toxicodynamic parameters relating to the larval response to exposure over time. We found that across the three repeats studies there was no more than a threefold difference in LC₅₀ values (13.1, 18.7 and 8.1 mg/Kg) at 48 h and fourfold difference at 96 h, highlighting the repeatability of the bioassay. We also highlighted the potential of the method to observe sub-lethal effects such as changes in weight. Finally, we discuss the applications of this new bioassay method to chemical risk assessments and its potential for use in other scenarios, such as mixture or pulsed exposure testing.http://www.sciencedirect.com/science/article/pii/S0147651323000088Cabbage MothInsecticideSurvival TestToxicokineticsToxicodynamicsEffect modelling |
| spellingShingle | Claire Badder Sylvain Bart Alex Robinson Helen Hesketh Peter Kille David J. Spurgeon A Novel Lepidoptera bioassay analysed using a reduced GUTS model Ecotoxicology and Environmental Safety Cabbage Moth Insecticide Survival Test Toxicokinetics Toxicodynamics Effect modelling |
| title | A Novel Lepidoptera bioassay analysed using a reduced GUTS model |
| title_full | A Novel Lepidoptera bioassay analysed using a reduced GUTS model |
| title_fullStr | A Novel Lepidoptera bioassay analysed using a reduced GUTS model |
| title_full_unstemmed | A Novel Lepidoptera bioassay analysed using a reduced GUTS model |
| title_short | A Novel Lepidoptera bioassay analysed using a reduced GUTS model |
| title_sort | novel lepidoptera bioassay analysed using a reduced guts model |
| topic | Cabbage Moth Insecticide Survival Test Toxicokinetics Toxicodynamics Effect modelling |
| url | http://www.sciencedirect.com/science/article/pii/S0147651323000088 |
| work_keys_str_mv | AT clairebadder anovellepidopterabioassayanalysedusingareducedgutsmodel AT sylvainbart anovellepidopterabioassayanalysedusingareducedgutsmodel AT alexrobinson anovellepidopterabioassayanalysedusingareducedgutsmodel AT helenhesketh anovellepidopterabioassayanalysedusingareducedgutsmodel AT peterkille anovellepidopterabioassayanalysedusingareducedgutsmodel AT davidjspurgeon anovellepidopterabioassayanalysedusingareducedgutsmodel AT clairebadder novellepidopterabioassayanalysedusingareducedgutsmodel AT sylvainbart novellepidopterabioassayanalysedusingareducedgutsmodel AT alexrobinson novellepidopterabioassayanalysedusingareducedgutsmodel AT helenhesketh novellepidopterabioassayanalysedusingareducedgutsmodel AT peterkille novellepidopterabioassayanalysedusingareducedgutsmodel AT davidjspurgeon novellepidopterabioassayanalysedusingareducedgutsmodel |