Entomological Surveillance for Zika and Dengue Virus in <i>Aedes</i> Mosquitoes: Implications for Vector Control in Thailand
Entomological surveillance for arthropod-borne viruses is vital for monitoring vector-borne diseases and informing vector control programs. In this study, we conducted entomological surveillance in Zika virus endemic areas. In Thailand, it is standard protocol to perform mosquito control within 24 h...
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MDPI AG
2020-06-01
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| Series: | Pathogens |
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| Online Access: | https://www.mdpi.com/2076-0817/9/6/442 |
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| author | Nathamon Kosoltanapiwat Jarinee Tongshoob Preeraya Singkhaimuk Chanyapat Nitatsukprasert Silas A. Davidson Alongkot Ponlawat |
| author_facet | Nathamon Kosoltanapiwat Jarinee Tongshoob Preeraya Singkhaimuk Chanyapat Nitatsukprasert Silas A. Davidson Alongkot Ponlawat |
| author_sort | Nathamon Kosoltanapiwat |
| collection | DOAJ |
| description | Entomological surveillance for arthropod-borne viruses is vital for monitoring vector-borne diseases and informing vector control programs. In this study, we conducted entomological surveillance in Zika virus endemic areas. In Thailand, it is standard protocol to perform mosquito control within 24 h of a reported dengue case. <i>Aedes</i> females were collected within 72 h of case reports from villages with recent Zika–human cases in Kamphaeng Phet Province, Thailand in 2017 and 2018. Mosquitoes were bisected into head-thorax and abdomen and then screened for Zika (ZIKV) and dengue (DENV) viruses using real-time RT-PCR. ZIKV RNA was detected in three samples from two female <i>Ae. aegypti</i> (1.4%). A partial envelope sequence analysis revealed that the ZIKV sequences were the Asian lineage identical to sequences from ZIKV-infected cases reported in Thailand during 2016 and 2017. Dengue virus-1 (DENV-1) and dengue virus-4 (DENV-4) were found in four <i>Ae. aegypti</i> females (2.8%), and partial capsid sequences were nearly identical with DENV-1 and DENV-4 from Thai human cases reported in 2017. Findings in the current study demonstrate the importance of entomological surveillance programs to public health mosquito-borne disease prevention measures and control. |
| first_indexed | 2024-03-10T19:22:31Z |
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| institution | Directory Open Access Journal |
| issn | 2076-0817 |
| language | English |
| last_indexed | 2024-03-10T19:22:31Z |
| publishDate | 2020-06-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Pathogens |
| spelling | doaj.art-213a42bf3ac448f585b9e5171fe8e18f2023-11-20T02:50:55ZengMDPI AGPathogens2076-08172020-06-019644210.3390/pathogens9060442Entomological Surveillance for Zika and Dengue Virus in <i>Aedes</i> Mosquitoes: Implications for Vector Control in ThailandNathamon Kosoltanapiwat0Jarinee Tongshoob1Preeraya Singkhaimuk2Chanyapat Nitatsukprasert3Silas A. Davidson4Alongkot Ponlawat5Department of Microbiology and Immunology, Faculty of Tropical Medicine, Mahidol University, Bangkok 10400, ThailandDepartment of Microbiology and Immunology, Faculty of Tropical Medicine, Mahidol University, Bangkok 10400, ThailandDepartment of Entomology, Armed Forces Research Institute of Medical Sciences (AFRIMS), Bangkok 10400, ThailandDepartment of Entomology, Armed Forces Research Institute of Medical Sciences (AFRIMS), Bangkok 10400, ThailandDepartment of Entomology, Armed Forces Research Institute of Medical Sciences (AFRIMS), Bangkok 10400, ThailandDepartment of Entomology, Armed Forces Research Institute of Medical Sciences (AFRIMS), Bangkok 10400, ThailandEntomological surveillance for arthropod-borne viruses is vital for monitoring vector-borne diseases and informing vector control programs. In this study, we conducted entomological surveillance in Zika virus endemic areas. In Thailand, it is standard protocol to perform mosquito control within 24 h of a reported dengue case. <i>Aedes</i> females were collected within 72 h of case reports from villages with recent Zika–human cases in Kamphaeng Phet Province, Thailand in 2017 and 2018. Mosquitoes were bisected into head-thorax and abdomen and then screened for Zika (ZIKV) and dengue (DENV) viruses using real-time RT-PCR. ZIKV RNA was detected in three samples from two female <i>Ae. aegypti</i> (1.4%). A partial envelope sequence analysis revealed that the ZIKV sequences were the Asian lineage identical to sequences from ZIKV-infected cases reported in Thailand during 2016 and 2017. Dengue virus-1 (DENV-1) and dengue virus-4 (DENV-4) were found in four <i>Ae. aegypti</i> females (2.8%), and partial capsid sequences were nearly identical with DENV-1 and DENV-4 from Thai human cases reported in 2017. Findings in the current study demonstrate the importance of entomological surveillance programs to public health mosquito-borne disease prevention measures and control.https://www.mdpi.com/2076-0817/9/6/442zika virusdengue virus<i>Aedes aegypti</i>mosquito surveillanceThailand |
| spellingShingle | Nathamon Kosoltanapiwat Jarinee Tongshoob Preeraya Singkhaimuk Chanyapat Nitatsukprasert Silas A. Davidson Alongkot Ponlawat Entomological Surveillance for Zika and Dengue Virus in <i>Aedes</i> Mosquitoes: Implications for Vector Control in Thailand Pathogens zika virus dengue virus <i>Aedes aegypti</i> mosquito surveillance Thailand |
| title | Entomological Surveillance for Zika and Dengue Virus in <i>Aedes</i> Mosquitoes: Implications for Vector Control in Thailand |
| title_full | Entomological Surveillance for Zika and Dengue Virus in <i>Aedes</i> Mosquitoes: Implications for Vector Control in Thailand |
| title_fullStr | Entomological Surveillance for Zika and Dengue Virus in <i>Aedes</i> Mosquitoes: Implications for Vector Control in Thailand |
| title_full_unstemmed | Entomological Surveillance for Zika and Dengue Virus in <i>Aedes</i> Mosquitoes: Implications for Vector Control in Thailand |
| title_short | Entomological Surveillance for Zika and Dengue Virus in <i>Aedes</i> Mosquitoes: Implications for Vector Control in Thailand |
| title_sort | entomological surveillance for zika and dengue virus in i aedes i mosquitoes implications for vector control in thailand |
| topic | zika virus dengue virus <i>Aedes aegypti</i> mosquito surveillance Thailand |
| url | https://www.mdpi.com/2076-0817/9/6/442 |
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