Collagenase mRNA Overexpression and Decreased Extracellular Matrix Components Are Early Events in the Pathogenesis of Emphysema.
To describe the progression of parenchymal remodeling and metalloproteinases gene expression in earlier stages of emphysema, mice received porcine pancreatic elastase (PPE) instillation and Control groups received saline solution. After PPE instillation (1, 3, 6 hours, 3 and 21 days) we measured the...
| Main Authors: | , , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Public Library of Science (PLoS)
2015-01-01
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| Series: | PLoS ONE |
| Online Access: | https://doi.org/10.1371/journal.pone.0129590 |
| _version_ | 1818588762956890112 |
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| author | Fabíola S Z Robertoni Clarice R Olivo Juliana D Lourenço Natália G Gonçalves Ana Paula P Velosa Chin J Lin Cláudia M Fló Beatriz M Saraiva-Romanholo Sérgio D Sasaki Milton A Martins Walcy R Teodoro Fernanda Degobbi T Q S Lopes |
| author_facet | Fabíola S Z Robertoni Clarice R Olivo Juliana D Lourenço Natália G Gonçalves Ana Paula P Velosa Chin J Lin Cláudia M Fló Beatriz M Saraiva-Romanholo Sérgio D Sasaki Milton A Martins Walcy R Teodoro Fernanda Degobbi T Q S Lopes |
| author_sort | Fabíola S Z Robertoni |
| collection | DOAJ |
| description | To describe the progression of parenchymal remodeling and metalloproteinases gene expression in earlier stages of emphysema, mice received porcine pancreatic elastase (PPE) instillation and Control groups received saline solution. After PPE instillation (1, 3, 6 hours, 3 and 21 days) we measured the mean linear intercept, the volume proportion of types I and III collagen, elastin, fibrillin and the MMP-1, -8, -12 and -13 gene expression. We observed an initial decrease in type I (at the 3rd day) and type III collagen (from the 6th hour until the 3rd day), in posterior time points in which we detected increased gene expression for MMP-8 and -13 in PPE groups. After 21 days, the type III collagen fibers increased and the type I collagen values returned to similar values compared to control groups. The MMP-12 gene expression was increased in earlier times (3 and 6 hours) to which we detected a reduced proportion of elastin (3 days) in PPE groups, reinforcing the already established importance of MMP-12 in the breakdown of ECM. Such findings will be useful to better elucidate the alterations in ECM components and the importance of not only metalloelastase but also collagenases in earlier emphysema stages, providing new clues to novel therapeutic targets. |
| first_indexed | 2024-12-16T09:29:55Z |
| format | Article |
| id | doaj.art-213b85069b5a47c3be8c93a9cef49008 |
| institution | Directory Open Access Journal |
| issn | 1932-6203 |
| language | English |
| last_indexed | 2024-12-16T09:29:55Z |
| publishDate | 2015-01-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS ONE |
| spelling | doaj.art-213b85069b5a47c3be8c93a9cef490082022-12-21T22:36:33ZengPublic Library of Science (PLoS)PLoS ONE1932-62032015-01-01106e012959010.1371/journal.pone.0129590Collagenase mRNA Overexpression and Decreased Extracellular Matrix Components Are Early Events in the Pathogenesis of Emphysema.Fabíola S Z RobertoniClarice R OlivoJuliana D LourençoNatália G GonçalvesAna Paula P VelosaChin J LinCláudia M FlóBeatriz M Saraiva-RomanholoSérgio D SasakiMilton A MartinsWalcy R TeodoroFernanda Degobbi T Q S LopesTo describe the progression of parenchymal remodeling and metalloproteinases gene expression in earlier stages of emphysema, mice received porcine pancreatic elastase (PPE) instillation and Control groups received saline solution. After PPE instillation (1, 3, 6 hours, 3 and 21 days) we measured the mean linear intercept, the volume proportion of types I and III collagen, elastin, fibrillin and the MMP-1, -8, -12 and -13 gene expression. We observed an initial decrease in type I (at the 3rd day) and type III collagen (from the 6th hour until the 3rd day), in posterior time points in which we detected increased gene expression for MMP-8 and -13 in PPE groups. After 21 days, the type III collagen fibers increased and the type I collagen values returned to similar values compared to control groups. The MMP-12 gene expression was increased in earlier times (3 and 6 hours) to which we detected a reduced proportion of elastin (3 days) in PPE groups, reinforcing the already established importance of MMP-12 in the breakdown of ECM. Such findings will be useful to better elucidate the alterations in ECM components and the importance of not only metalloelastase but also collagenases in earlier emphysema stages, providing new clues to novel therapeutic targets.https://doi.org/10.1371/journal.pone.0129590 |
| spellingShingle | Fabíola S Z Robertoni Clarice R Olivo Juliana D Lourenço Natália G Gonçalves Ana Paula P Velosa Chin J Lin Cláudia M Fló Beatriz M Saraiva-Romanholo Sérgio D Sasaki Milton A Martins Walcy R Teodoro Fernanda Degobbi T Q S Lopes Collagenase mRNA Overexpression and Decreased Extracellular Matrix Components Are Early Events in the Pathogenesis of Emphysema. PLoS ONE |
| title | Collagenase mRNA Overexpression and Decreased Extracellular Matrix Components Are Early Events in the Pathogenesis of Emphysema. |
| title_full | Collagenase mRNA Overexpression and Decreased Extracellular Matrix Components Are Early Events in the Pathogenesis of Emphysema. |
| title_fullStr | Collagenase mRNA Overexpression and Decreased Extracellular Matrix Components Are Early Events in the Pathogenesis of Emphysema. |
| title_full_unstemmed | Collagenase mRNA Overexpression and Decreased Extracellular Matrix Components Are Early Events in the Pathogenesis of Emphysema. |
| title_short | Collagenase mRNA Overexpression and Decreased Extracellular Matrix Components Are Early Events in the Pathogenesis of Emphysema. |
| title_sort | collagenase mrna overexpression and decreased extracellular matrix components are early events in the pathogenesis of emphysema |
| url | https://doi.org/10.1371/journal.pone.0129590 |
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