Monodisperse Water-in-Oil-in-Water (W/O/W) Double Emulsion Droplets as Uniform Compartments for High-Throughput Analysis via Flow Cytometry
Here we report the application of monodisperse double emulsion droplets, produced in a single step within partially hydrophilic/partially hydrophobic microfluidic devices, as defined containers for quantitative flow cytometric analysis. Samples with varying fluorophore concentrations were generated,...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2013-12-01
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| Series: | Micromachines |
| Subjects: | |
| Online Access: | http://www.mdpi.com/2072-666X/4/4/402 |
| _version_ | 1818557349150851072 |
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| author | Jing Yan Wolfgang-Andreas C. Bauer Martin Fischlechner Florian Hollfelder Clemens F. Kaminski Wilhelm T. S. Huck |
| author_facet | Jing Yan Wolfgang-Andreas C. Bauer Martin Fischlechner Florian Hollfelder Clemens F. Kaminski Wilhelm T. S. Huck |
| author_sort | Jing Yan |
| collection | DOAJ |
| description | Here we report the application of monodisperse double emulsion droplets, produced in a single step within partially hydrophilic/partially hydrophobic microfluidic devices, as defined containers for quantitative flow cytometric analysis. Samples with varying fluorophore concentrations were generated, and a clear correlation between dye concentration and fluorescence signals was observed. |
| first_indexed | 2024-12-13T23:58:20Z |
| format | Article |
| id | doaj.art-214268c6a12c4dbd85e28f1addeab3a2 |
| institution | Directory Open Access Journal |
| issn | 2072-666X |
| language | English |
| last_indexed | 2024-12-13T23:58:20Z |
| publishDate | 2013-12-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Micromachines |
| spelling | doaj.art-214268c6a12c4dbd85e28f1addeab3a22022-12-21T23:26:27ZengMDPI AGMicromachines2072-666X2013-12-014440241310.3390/mi4040402mi4040402Monodisperse Water-in-Oil-in-Water (W/O/W) Double Emulsion Droplets as Uniform Compartments for High-Throughput Analysis via Flow CytometryJing Yan0Wolfgang-Andreas C. Bauer1Martin Fischlechner2Florian Hollfelder3Clemens F. Kaminski4Wilhelm T. S. Huck5Department of Biochemistry, University of Cambridge, 80 Tennis Court Road, Cambridge CB2 1GA, UKMelville Laboratory for Polymer Synthesis, Department of Chemistry, University of Cambridge, Lensfield Road, Cambridge CB2 1EW, UKInstitute for Life Sciences, University of Southampton, Highfield Campus, Southampton SO17 1BJ, UKDepartment of Biochemistry, University of Cambridge, 80 Tennis Court Road, Cambridge CB2 1GA, UKDepartment of Chemical Engineering and Biotechnology, University of Cambridge, Pembroke Street, Cambridge CB2 3RA, UKMelville Laboratory for Polymer Synthesis, Department of Chemistry, University of Cambridge, Lensfield Road, Cambridge CB2 1EW, UKHere we report the application of monodisperse double emulsion droplets, produced in a single step within partially hydrophilic/partially hydrophobic microfluidic devices, as defined containers for quantitative flow cytometric analysis. Samples with varying fluorophore concentrations were generated, and a clear correlation between dye concentration and fluorescence signals was observed.http://www.mdpi.com/2072-666X/4/4/402microfluidicsdouble emulsiondouble flow-focusing geometryflow cytometry |
| spellingShingle | Jing Yan Wolfgang-Andreas C. Bauer Martin Fischlechner Florian Hollfelder Clemens F. Kaminski Wilhelm T. S. Huck Monodisperse Water-in-Oil-in-Water (W/O/W) Double Emulsion Droplets as Uniform Compartments for High-Throughput Analysis via Flow Cytometry Micromachines microfluidics double emulsion double flow-focusing geometry flow cytometry |
| title | Monodisperse Water-in-Oil-in-Water (W/O/W) Double Emulsion Droplets as Uniform Compartments for High-Throughput Analysis via Flow Cytometry |
| title_full | Monodisperse Water-in-Oil-in-Water (W/O/W) Double Emulsion Droplets as Uniform Compartments for High-Throughput Analysis via Flow Cytometry |
| title_fullStr | Monodisperse Water-in-Oil-in-Water (W/O/W) Double Emulsion Droplets as Uniform Compartments for High-Throughput Analysis via Flow Cytometry |
| title_full_unstemmed | Monodisperse Water-in-Oil-in-Water (W/O/W) Double Emulsion Droplets as Uniform Compartments for High-Throughput Analysis via Flow Cytometry |
| title_short | Monodisperse Water-in-Oil-in-Water (W/O/W) Double Emulsion Droplets as Uniform Compartments for High-Throughput Analysis via Flow Cytometry |
| title_sort | monodisperse water in oil in water w o w double emulsion droplets as uniform compartments for high throughput analysis via flow cytometry |
| topic | microfluidics double emulsion double flow-focusing geometry flow cytometry |
| url | http://www.mdpi.com/2072-666X/4/4/402 |
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