Bach2 overexpression represses Th9 cell differentiation by suppressing IRF4 expression in systemic lupus erythematosus
Systemic lupus erythematosus (SLE) is a chronic autoimmune disease characterized by abnormal activation of T cells and caused by an imbalance in the production and clearance of apoptotic cells. We previously showed that the transcription regulator Bach2 regulated abnormal B‐cell activation in SLE. H...
| Main Authors: | , , , , , , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Wiley
2021-02-01
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| Series: | FEBS Open Bio |
| Subjects: | |
| Online Access: | https://doi.org/10.1002/2211-5463.13050 |
| _version_ | 1811178372445765632 |
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| author | Yujun Sheng Jiali Zhang Keke Li Hongyan Wang Wenjun Wang Leilei Wen Jinping Gao Xianfa Tang Huayang Tang He Huang Minglong Cai Tao Yuan Lu Liu Xiaodong Zheng Zhengwei Zhu Yong Cui |
| author_facet | Yujun Sheng Jiali Zhang Keke Li Hongyan Wang Wenjun Wang Leilei Wen Jinping Gao Xianfa Tang Huayang Tang He Huang Minglong Cai Tao Yuan Lu Liu Xiaodong Zheng Zhengwei Zhu Yong Cui |
| author_sort | Yujun Sheng |
| collection | DOAJ |
| description | Systemic lupus erythematosus (SLE) is a chronic autoimmune disease characterized by abnormal activation of T cells and caused by an imbalance in the production and clearance of apoptotic cells. We previously showed that the transcription regulator Bach2 regulated abnormal B‐cell activation in SLE. Here, we investigated whether Bach2 was also involved in Th9 cell differentiation in SLE. We found that the proportion of Th9 cells was enhanced in the peripheral blood mononuclear cells (PBMC) of SLE patients. The PBMC and CD4+ T cells of SLE patients exhibited a decrease of Bach2 expression and an increase of IL‐9 expression. Furthermore, Bach2 overexpression significantly repressed the levels of PU.1, IRF4, IL‐9, and Th9 cells in the CD4+ T cells of SLE patients and healthy volunteers. In addition, Bach2 overexpression inhibited the levels of IL‐9 and Th9 cells, whereas IRF4 upregulation enhanced the levels of IRF4 and IL‐9 and Th9 cells in the CD4+ T cells of SLE patients and healthy volunteers. The effect of IRF4 up‐regulation was abolished by Bach2 overexpression. In summary, our work suggests that Bach2 overexpression represses Th9 cell differentiation by suppressing IRF4 expression in SLE, and thus, Bach2 may be a novel potential target for SLE treatment. |
| first_indexed | 2024-04-11T06:17:06Z |
| format | Article |
| id | doaj.art-2143e338bf8040c5978eaf4f37243b3b |
| institution | Directory Open Access Journal |
| issn | 2211-5463 |
| language | English |
| last_indexed | 2024-04-11T06:17:06Z |
| publishDate | 2021-02-01 |
| publisher | Wiley |
| record_format | Article |
| series | FEBS Open Bio |
| spelling | doaj.art-2143e338bf8040c5978eaf4f37243b3b2022-12-22T04:41:01ZengWileyFEBS Open Bio2211-54632021-02-0111239540310.1002/2211-5463.13050Bach2 overexpression represses Th9 cell differentiation by suppressing IRF4 expression in systemic lupus erythematosusYujun Sheng0Jiali Zhang1Keke Li2Hongyan Wang3Wenjun Wang4Leilei Wen5Jinping Gao6Xianfa Tang7Huayang Tang8He Huang9Minglong Cai10Tao Yuan11Lu Liu12Xiaodong Zheng13Zhengwei Zhu14Yong Cui15Department of Dermatology Institute of Dermatology the First Affiliated Hospital Anhui Medical University Hefei ChinaDepartment of Dermatology Institute of Dermatology the First Affiliated Hospital Anhui Medical University Hefei ChinaDepartment of Dermatology China–Japan Friendship Hospital Beijing ChinaDepartment of Dermatology Institute of Dermatology the First Affiliated Hospital Anhui Medical University Hefei ChinaDepartment of Dermatology Institute of Dermatology the First Affiliated Hospital Anhui Medical University Hefei ChinaDepartment of Dermatology Institute of Dermatology the First Affiliated Hospital Anhui Medical University Hefei ChinaDepartment of Dermatology Institute of Dermatology the First Affiliated Hospital Anhui Medical University Hefei ChinaDepartment of Dermatology Institute of Dermatology the First Affiliated Hospital Anhui Medical University Hefei ChinaDepartment of Dermatology Institute of Dermatology the First Affiliated Hospital Anhui Medical University Hefei ChinaDepartment of Dermatology Institute of Dermatology the First Affiliated Hospital Anhui Medical University Hefei ChinaDepartment of Dermatology Institute of Dermatology the First Affiliated Hospital Anhui Medical University Hefei ChinaDepartment of Dermatology Institute of Dermatology the First Affiliated Hospital Anhui Medical University Hefei ChinaDepartment of Dermatology Institute of Dermatology the First Affiliated Hospital Anhui Medical University Hefei ChinaDepartment of Dermatology Institute of Dermatology the First Affiliated Hospital Anhui Medical University Hefei ChinaDepartment of Dermatology Institute of Dermatology the First Affiliated Hospital Anhui Medical University Hefei ChinaDepartment of Dermatology China–Japan Friendship Hospital Beijing ChinaSystemic lupus erythematosus (SLE) is a chronic autoimmune disease characterized by abnormal activation of T cells and caused by an imbalance in the production and clearance of apoptotic cells. We previously showed that the transcription regulator Bach2 regulated abnormal B‐cell activation in SLE. Here, we investigated whether Bach2 was also involved in Th9 cell differentiation in SLE. We found that the proportion of Th9 cells was enhanced in the peripheral blood mononuclear cells (PBMC) of SLE patients. The PBMC and CD4+ T cells of SLE patients exhibited a decrease of Bach2 expression and an increase of IL‐9 expression. Furthermore, Bach2 overexpression significantly repressed the levels of PU.1, IRF4, IL‐9, and Th9 cells in the CD4+ T cells of SLE patients and healthy volunteers. In addition, Bach2 overexpression inhibited the levels of IL‐9 and Th9 cells, whereas IRF4 upregulation enhanced the levels of IRF4 and IL‐9 and Th9 cells in the CD4+ T cells of SLE patients and healthy volunteers. The effect of IRF4 up‐regulation was abolished by Bach2 overexpression. In summary, our work suggests that Bach2 overexpression represses Th9 cell differentiation by suppressing IRF4 expression in SLE, and thus, Bach2 may be a novel potential target for SLE treatment.https://doi.org/10.1002/2211-5463.13050Bach2CD4+ T cellsIL‐9systemic lupus erythematosusTh9 cells |
| spellingShingle | Yujun Sheng Jiali Zhang Keke Li Hongyan Wang Wenjun Wang Leilei Wen Jinping Gao Xianfa Tang Huayang Tang He Huang Minglong Cai Tao Yuan Lu Liu Xiaodong Zheng Zhengwei Zhu Yong Cui Bach2 overexpression represses Th9 cell differentiation by suppressing IRF4 expression in systemic lupus erythematosus FEBS Open Bio Bach2 CD4+ T cells IL‐9 systemic lupus erythematosus Th9 cells |
| title | Bach2 overexpression represses Th9 cell differentiation by suppressing IRF4 expression in systemic lupus erythematosus |
| title_full | Bach2 overexpression represses Th9 cell differentiation by suppressing IRF4 expression in systemic lupus erythematosus |
| title_fullStr | Bach2 overexpression represses Th9 cell differentiation by suppressing IRF4 expression in systemic lupus erythematosus |
| title_full_unstemmed | Bach2 overexpression represses Th9 cell differentiation by suppressing IRF4 expression in systemic lupus erythematosus |
| title_short | Bach2 overexpression represses Th9 cell differentiation by suppressing IRF4 expression in systemic lupus erythematosus |
| title_sort | bach2 overexpression represses th9 cell differentiation by suppressing irf4 expression in systemic lupus erythematosus |
| topic | Bach2 CD4+ T cells IL‐9 systemic lupus erythematosus Th9 cells |
| url | https://doi.org/10.1002/2211-5463.13050 |
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