Evaluation of Acute and Subacute Toxicity and LC-MS/MS Compositional Alkaloid Determination of the Hydroethanolic Extract of <i>Dysphania ambrosioides</i> (L.) Mosyakin and Clemants Flowers
<i>Dysphania ambrosioides</i> (L.) Mosyakin and Clemants is a medicinal plant that has traditionally been used to cure a range of diseases. There has been no thorough investigation of the potential toxicity of this plant. The objective of this study is to assess the acute and subacute to...
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MDPI AG
2022-07-01
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| Series: | Toxins |
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| Online Access: | https://www.mdpi.com/2072-6651/14/7/475 |
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| author | Fahd Kandsi Fatima Zahra Lafdil Amine Elbouzidi Saliha Bouknana Achraf Miry Mohamed Addi Raffaele Conte Christophe Hano Nadia Gseyra |
| author_facet | Fahd Kandsi Fatima Zahra Lafdil Amine Elbouzidi Saliha Bouknana Achraf Miry Mohamed Addi Raffaele Conte Christophe Hano Nadia Gseyra |
| author_sort | Fahd Kandsi |
| collection | DOAJ |
| description | <i>Dysphania ambrosioides</i> (L.) Mosyakin and Clemants is a medicinal plant that has traditionally been used to cure a range of diseases. There has been no thorough investigation of the potential toxicity of this plant. The objective of this study is to assess the acute and subacute toxicity of <i>D. ambrosioides</i> hydroethanolic extract (DAHE), as well as it alkaloids composition, utilizing LC-MS/MS analysis. An in silico approach was applied to determine pharmacokinetic parameters and to predict the toxicity of <i>D. ambrosioides</i> identified alkaloids. A 14-day treatment with a single oral dose of 1–7 g/kg was carried out to investigate acute toxicity. DAHE was given orally at dosages of 5, 50, and 500 mg/kg for 15 days in the subacute toxicity investigation, and body weight and biochemical parameters were evaluated. Livers, kidneys, lungs, and heart were examined histologically. Chromatographic investigation revealed the existence of nine alkaloids, with N-formylnorgalanthamine being the most prevalent. The oral LD<sub>50</sub> value of DAHE was found to be 5000 mg/kg in an acute toxicity study. No variations were observed with respect to food intake, water consumption, mortality, or body and organ weight in the subacute toxicity study. On the other hand, DAHE (500 mg/kg) significantly enhanced alanineaminotransferase, aspartate aminotransferase, and urea. Liver and kidney histological examinations revealed modest infiltration of hepatocyte trabeculae by inflammatory cells in the liver and slight alteration in the kidney histoarchitecture. According to our findings, DAHE exhibits low to moderate toxicity. |
| first_indexed | 2024-03-09T05:45:49Z |
| format | Article |
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| institution | Directory Open Access Journal |
| issn | 2072-6651 |
| language | English |
| last_indexed | 2024-03-09T05:45:49Z |
| publishDate | 2022-07-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Toxins |
| spelling | doaj.art-2145ac5f45774cc78dcc435c898bd62e2023-12-03T12:21:11ZengMDPI AGToxins2072-66512022-07-0114747510.3390/toxins14070475Evaluation of Acute and Subacute Toxicity and LC-MS/MS Compositional Alkaloid Determination of the Hydroethanolic Extract of <i>Dysphania ambrosioides</i> (L.) Mosyakin and Clemants FlowersFahd Kandsi0Fatima Zahra Lafdil1Amine Elbouzidi2Saliha Bouknana3Achraf Miry4Mohamed Addi5Raffaele Conte6Christophe Hano7Nadia Gseyra8Laboratory of Bioresources, Biotechnology, Ethnopharmacology and Health, Faculty of Sciences, Mohammed First University, B.P. 717, Oujda 60000, MoroccoLaboratory of Bioresources, Biotechnology, Ethnopharmacology and Health, Faculty of Sciences, Mohammed First University, B.P. 717, Oujda 60000, MoroccoLaboratoire d’Amélioration des Productions Agricoles, Biotechnologies’ et Environnement (LAPABE), Faculté des Sciences, Université Mohammed Premier, Oujda 60040, MoroccoLaboratory of Bioresources, Biotechnology, Ethnopharmacology and Health, Faculty of Sciences, Mohammed First University, B.P. 717, Oujda 60000, MoroccoPathology Department, Mohammed VI University Hospital, Oujda 60040, MoroccoLaboratoire d’Amélioration des Productions Agricoles, Biotechnologies’ et Environnement (LAPABE), Faculté des Sciences, Université Mohammed Premier, Oujda 60040, MoroccoResearch Institute on Terrestrial Ecosystems (IRET)—CNR, Via Pietro Castellano 111, 80131 Naples, ItalyLaboratoire de Biologie des Ligneux et des Grandes Cultures, INRAE USC1328, University of Orleans, CEDEX 2, 45067 Orléans, FranceLaboratory of Bioresources, Biotechnology, Ethnopharmacology and Health, Faculty of Sciences, Mohammed First University, B.P. 717, Oujda 60000, Morocco<i>Dysphania ambrosioides</i> (L.) Mosyakin and Clemants is a medicinal plant that has traditionally been used to cure a range of diseases. There has been no thorough investigation of the potential toxicity of this plant. The objective of this study is to assess the acute and subacute toxicity of <i>D. ambrosioides</i> hydroethanolic extract (DAHE), as well as it alkaloids composition, utilizing LC-MS/MS analysis. An in silico approach was applied to determine pharmacokinetic parameters and to predict the toxicity of <i>D. ambrosioides</i> identified alkaloids. A 14-day treatment with a single oral dose of 1–7 g/kg was carried out to investigate acute toxicity. DAHE was given orally at dosages of 5, 50, and 500 mg/kg for 15 days in the subacute toxicity investigation, and body weight and biochemical parameters were evaluated. Livers, kidneys, lungs, and heart were examined histologically. Chromatographic investigation revealed the existence of nine alkaloids, with N-formylnorgalanthamine being the most prevalent. The oral LD<sub>50</sub> value of DAHE was found to be 5000 mg/kg in an acute toxicity study. No variations were observed with respect to food intake, water consumption, mortality, or body and organ weight in the subacute toxicity study. On the other hand, DAHE (500 mg/kg) significantly enhanced alanineaminotransferase, aspartate aminotransferase, and urea. Liver and kidney histological examinations revealed modest infiltration of hepatocyte trabeculae by inflammatory cells in the liver and slight alteration in the kidney histoarchitecture. According to our findings, DAHE exhibits low to moderate toxicity.https://www.mdpi.com/2072-6651/14/7/475<i>Dysphania ambrosioides</i>Mexican teaalkaloidsLC-MS/MSacute toxicitysubacute toxicity |
| spellingShingle | Fahd Kandsi Fatima Zahra Lafdil Amine Elbouzidi Saliha Bouknana Achraf Miry Mohamed Addi Raffaele Conte Christophe Hano Nadia Gseyra Evaluation of Acute and Subacute Toxicity and LC-MS/MS Compositional Alkaloid Determination of the Hydroethanolic Extract of <i>Dysphania ambrosioides</i> (L.) Mosyakin and Clemants Flowers Toxins <i>Dysphania ambrosioides</i> Mexican tea alkaloids LC-MS/MS acute toxicity subacute toxicity |
| title | Evaluation of Acute and Subacute Toxicity and LC-MS/MS Compositional Alkaloid Determination of the Hydroethanolic Extract of <i>Dysphania ambrosioides</i> (L.) Mosyakin and Clemants Flowers |
| title_full | Evaluation of Acute and Subacute Toxicity and LC-MS/MS Compositional Alkaloid Determination of the Hydroethanolic Extract of <i>Dysphania ambrosioides</i> (L.) Mosyakin and Clemants Flowers |
| title_fullStr | Evaluation of Acute and Subacute Toxicity and LC-MS/MS Compositional Alkaloid Determination of the Hydroethanolic Extract of <i>Dysphania ambrosioides</i> (L.) Mosyakin and Clemants Flowers |
| title_full_unstemmed | Evaluation of Acute and Subacute Toxicity and LC-MS/MS Compositional Alkaloid Determination of the Hydroethanolic Extract of <i>Dysphania ambrosioides</i> (L.) Mosyakin and Clemants Flowers |
| title_short | Evaluation of Acute and Subacute Toxicity and LC-MS/MS Compositional Alkaloid Determination of the Hydroethanolic Extract of <i>Dysphania ambrosioides</i> (L.) Mosyakin and Clemants Flowers |
| title_sort | evaluation of acute and subacute toxicity and lc ms ms compositional alkaloid determination of the hydroethanolic extract of i dysphania ambrosioides i l mosyakin and clemants flowers |
| topic | <i>Dysphania ambrosioides</i> Mexican tea alkaloids LC-MS/MS acute toxicity subacute toxicity |
| url | https://www.mdpi.com/2072-6651/14/7/475 |
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