Network Pharmacology Analysis of Liquid-Cultured <i>Armillaria ostoyae</i> Mycelial Metabolites and Their Molecular Mechanism of Action against Gastric Cancer
<i>Armillaria</i> sp. are traditional edible medicinal mushrooms with various health functions; however, the relationship between their composition and efficacy has not yet been determined. Here, the ethanol extract of liquid-cultured <i>Armillaria ostoyae</i> mycelia (AOME),...
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2024-04-01
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author | Zhishuo Wang Ruiqi Wang Zhiguo Na Shanshan Liang Fan Wu Hongyao Xie Xue Zhang Wei Xu Xin Wang |
author_facet | Zhishuo Wang Ruiqi Wang Zhiguo Na Shanshan Liang Fan Wu Hongyao Xie Xue Zhang Wei Xu Xin Wang |
author_sort | Zhishuo Wang |
collection | DOAJ |
description | <i>Armillaria</i> sp. are traditional edible medicinal mushrooms with various health functions; however, the relationship between their composition and efficacy has not yet been determined. Here, the ethanol extract of liquid-cultured <i>Armillaria ostoyae</i> mycelia (AOME), a pure wild <i>Armillaria</i> sp. strain, was analyzed using UHPLC-QTOF/MS, network pharmacology, and molecular docking techniques. The obtained extract affects various metabolic pathways, such as JAK/STAT and PI3K/AKT. The extract also contains important compounds such as 4-(dimethylamino)-N-[7-(hydroxyamino)-7-oxoheptyl] benzamide, isoliquiritigenin, and 7-hydroxycoumarin. Moreover, the extract targets key proteins, including EGFR, SCR, and IL6, to suppress the progression of gastric cancer, thereby synergistically inhibiting cancer development. The molecular docking analyses indicated that the main compounds stably bind to the target proteins. The final cell culture experimental data showed that the ethanol extract inhibited MGC-803 gastric cancer cells. In summary, our research revealed the beneficial components of AOME for treating gastric cancer and its associated molecular pathways. However, further research is needed to confirm its effectiveness and safety in gastric cancer patients. |
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spelling | doaj.art-2150fccaa2724163b9b2854a0234dc622024-04-12T13:23:43ZengMDPI AGMolecules1420-30492024-04-01297166810.3390/molecules29071668Network Pharmacology Analysis of Liquid-Cultured <i>Armillaria ostoyae</i> Mycelial Metabolites and Their Molecular Mechanism of Action against Gastric CancerZhishuo Wang0Ruiqi Wang1Zhiguo Na2Shanshan Liang3Fan Wu4Hongyao Xie5Xue Zhang6Wei Xu7Xin Wang8School of Food Engineering, Harbin University of Commerce, Harbin 150028, ChinaSchool of Food Engineering, Harbin University of Commerce, Harbin 150028, ChinaSchool of Food Engineering, Harbin University of Commerce, Harbin 150028, ChinaSchool of Food Engineering, Harbin University of Commerce, Harbin 150028, ChinaSchool of Food Engineering, Harbin University of Commerce, Harbin 150028, ChinaSchool of Food Engineering, Harbin University of Commerce, Harbin 150028, ChinaSchool of Food Engineering, Harbin University of Commerce, Harbin 150028, ChinaSchool of Food Engineering, Harbin University of Commerce, Harbin 150028, ChinaSchool of Food Engineering, Harbin University of Commerce, Harbin 150028, China<i>Armillaria</i> sp. are traditional edible medicinal mushrooms with various health functions; however, the relationship between their composition and efficacy has not yet been determined. Here, the ethanol extract of liquid-cultured <i>Armillaria ostoyae</i> mycelia (AOME), a pure wild <i>Armillaria</i> sp. strain, was analyzed using UHPLC-QTOF/MS, network pharmacology, and molecular docking techniques. The obtained extract affects various metabolic pathways, such as JAK/STAT and PI3K/AKT. The extract also contains important compounds such as 4-(dimethylamino)-N-[7-(hydroxyamino)-7-oxoheptyl] benzamide, isoliquiritigenin, and 7-hydroxycoumarin. Moreover, the extract targets key proteins, including EGFR, SCR, and IL6, to suppress the progression of gastric cancer, thereby synergistically inhibiting cancer development. The molecular docking analyses indicated that the main compounds stably bind to the target proteins. The final cell culture experimental data showed that the ethanol extract inhibited MGC-803 gastric cancer cells. In summary, our research revealed the beneficial components of AOME for treating gastric cancer and its associated molecular pathways. However, further research is needed to confirm its effectiveness and safety in gastric cancer patients.https://www.mdpi.com/1420-3049/29/7/1668<i>Armillaria ostoyae</i>liquid-cultured myceliumethanol extractnetwork pharmacologymolecular dockinggastric cancer |
spellingShingle | Zhishuo Wang Ruiqi Wang Zhiguo Na Shanshan Liang Fan Wu Hongyao Xie Xue Zhang Wei Xu Xin Wang Network Pharmacology Analysis of Liquid-Cultured <i>Armillaria ostoyae</i> Mycelial Metabolites and Their Molecular Mechanism of Action against Gastric Cancer Molecules <i>Armillaria ostoyae</i> liquid-cultured mycelium ethanol extract network pharmacology molecular docking gastric cancer |
title | Network Pharmacology Analysis of Liquid-Cultured <i>Armillaria ostoyae</i> Mycelial Metabolites and Their Molecular Mechanism of Action against Gastric Cancer |
title_full | Network Pharmacology Analysis of Liquid-Cultured <i>Armillaria ostoyae</i> Mycelial Metabolites and Their Molecular Mechanism of Action against Gastric Cancer |
title_fullStr | Network Pharmacology Analysis of Liquid-Cultured <i>Armillaria ostoyae</i> Mycelial Metabolites and Their Molecular Mechanism of Action against Gastric Cancer |
title_full_unstemmed | Network Pharmacology Analysis of Liquid-Cultured <i>Armillaria ostoyae</i> Mycelial Metabolites and Their Molecular Mechanism of Action against Gastric Cancer |
title_short | Network Pharmacology Analysis of Liquid-Cultured <i>Armillaria ostoyae</i> Mycelial Metabolites and Their Molecular Mechanism of Action against Gastric Cancer |
title_sort | network pharmacology analysis of liquid cultured i armillaria ostoyae i mycelial metabolites and their molecular mechanism of action against gastric cancer |
topic | <i>Armillaria ostoyae</i> liquid-cultured mycelium ethanol extract network pharmacology molecular docking gastric cancer |
url | https://www.mdpi.com/1420-3049/29/7/1668 |
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