Network Pharmacology Analysis of Liquid-Cultured <i>Armillaria ostoyae</i> Mycelial Metabolites and Their Molecular Mechanism of Action against Gastric Cancer

<i>Armillaria</i> sp. are traditional edible medicinal mushrooms with various health functions; however, the relationship between their composition and efficacy has not yet been determined. Here, the ethanol extract of liquid-cultured <i>Armillaria ostoyae</i> mycelia (AOME),...

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Main Authors: Zhishuo Wang, Ruiqi Wang, Zhiguo Na, Shanshan Liang, Fan Wu, Hongyao Xie, Xue Zhang, Wei Xu, Xin Wang
Format: Article
Language:English
Published: MDPI AG 2024-04-01
Series:Molecules
Subjects:
Online Access:https://www.mdpi.com/1420-3049/29/7/1668
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author Zhishuo Wang
Ruiqi Wang
Zhiguo Na
Shanshan Liang
Fan Wu
Hongyao Xie
Xue Zhang
Wei Xu
Xin Wang
author_facet Zhishuo Wang
Ruiqi Wang
Zhiguo Na
Shanshan Liang
Fan Wu
Hongyao Xie
Xue Zhang
Wei Xu
Xin Wang
author_sort Zhishuo Wang
collection DOAJ
description <i>Armillaria</i> sp. are traditional edible medicinal mushrooms with various health functions; however, the relationship between their composition and efficacy has not yet been determined. Here, the ethanol extract of liquid-cultured <i>Armillaria ostoyae</i> mycelia (AOME), a pure wild <i>Armillaria</i> sp. strain, was analyzed using UHPLC-QTOF/MS, network pharmacology, and molecular docking techniques. The obtained extract affects various metabolic pathways, such as JAK/STAT and PI3K/AKT. The extract also contains important compounds such as 4-(dimethylamino)-N-[7-(hydroxyamino)-7-oxoheptyl] benzamide, isoliquiritigenin, and 7-hydroxycoumarin. Moreover, the extract targets key proteins, including EGFR, SCR, and IL6, to suppress the progression of gastric cancer, thereby synergistically inhibiting cancer development. The molecular docking analyses indicated that the main compounds stably bind to the target proteins. The final cell culture experimental data showed that the ethanol extract inhibited MGC-803 gastric cancer cells. In summary, our research revealed the beneficial components of AOME for treating gastric cancer and its associated molecular pathways. However, further research is needed to confirm its effectiveness and safety in gastric cancer patients.
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spelling doaj.art-2150fccaa2724163b9b2854a0234dc622024-04-12T13:23:43ZengMDPI AGMolecules1420-30492024-04-01297166810.3390/molecules29071668Network Pharmacology Analysis of Liquid-Cultured <i>Armillaria ostoyae</i> Mycelial Metabolites and Their Molecular Mechanism of Action against Gastric CancerZhishuo Wang0Ruiqi Wang1Zhiguo Na2Shanshan Liang3Fan Wu4Hongyao Xie5Xue Zhang6Wei Xu7Xin Wang8School of Food Engineering, Harbin University of Commerce, Harbin 150028, ChinaSchool of Food Engineering, Harbin University of Commerce, Harbin 150028, ChinaSchool of Food Engineering, Harbin University of Commerce, Harbin 150028, ChinaSchool of Food Engineering, Harbin University of Commerce, Harbin 150028, ChinaSchool of Food Engineering, Harbin University of Commerce, Harbin 150028, ChinaSchool of Food Engineering, Harbin University of Commerce, Harbin 150028, ChinaSchool of Food Engineering, Harbin University of Commerce, Harbin 150028, ChinaSchool of Food Engineering, Harbin University of Commerce, Harbin 150028, ChinaSchool of Food Engineering, Harbin University of Commerce, Harbin 150028, China<i>Armillaria</i> sp. are traditional edible medicinal mushrooms with various health functions; however, the relationship between their composition and efficacy has not yet been determined. Here, the ethanol extract of liquid-cultured <i>Armillaria ostoyae</i> mycelia (AOME), a pure wild <i>Armillaria</i> sp. strain, was analyzed using UHPLC-QTOF/MS, network pharmacology, and molecular docking techniques. The obtained extract affects various metabolic pathways, such as JAK/STAT and PI3K/AKT. The extract also contains important compounds such as 4-(dimethylamino)-N-[7-(hydroxyamino)-7-oxoheptyl] benzamide, isoliquiritigenin, and 7-hydroxycoumarin. Moreover, the extract targets key proteins, including EGFR, SCR, and IL6, to suppress the progression of gastric cancer, thereby synergistically inhibiting cancer development. The molecular docking analyses indicated that the main compounds stably bind to the target proteins. The final cell culture experimental data showed that the ethanol extract inhibited MGC-803 gastric cancer cells. In summary, our research revealed the beneficial components of AOME for treating gastric cancer and its associated molecular pathways. However, further research is needed to confirm its effectiveness and safety in gastric cancer patients.https://www.mdpi.com/1420-3049/29/7/1668<i>Armillaria ostoyae</i>liquid-cultured myceliumethanol extractnetwork pharmacologymolecular dockinggastric cancer
spellingShingle Zhishuo Wang
Ruiqi Wang
Zhiguo Na
Shanshan Liang
Fan Wu
Hongyao Xie
Xue Zhang
Wei Xu
Xin Wang
Network Pharmacology Analysis of Liquid-Cultured <i>Armillaria ostoyae</i> Mycelial Metabolites and Their Molecular Mechanism of Action against Gastric Cancer
Molecules
<i>Armillaria ostoyae</i>
liquid-cultured mycelium
ethanol extract
network pharmacology
molecular docking
gastric cancer
title Network Pharmacology Analysis of Liquid-Cultured <i>Armillaria ostoyae</i> Mycelial Metabolites and Their Molecular Mechanism of Action against Gastric Cancer
title_full Network Pharmacology Analysis of Liquid-Cultured <i>Armillaria ostoyae</i> Mycelial Metabolites and Their Molecular Mechanism of Action against Gastric Cancer
title_fullStr Network Pharmacology Analysis of Liquid-Cultured <i>Armillaria ostoyae</i> Mycelial Metabolites and Their Molecular Mechanism of Action against Gastric Cancer
title_full_unstemmed Network Pharmacology Analysis of Liquid-Cultured <i>Armillaria ostoyae</i> Mycelial Metabolites and Their Molecular Mechanism of Action against Gastric Cancer
title_short Network Pharmacology Analysis of Liquid-Cultured <i>Armillaria ostoyae</i> Mycelial Metabolites and Their Molecular Mechanism of Action against Gastric Cancer
title_sort network pharmacology analysis of liquid cultured i armillaria ostoyae i mycelial metabolites and their molecular mechanism of action against gastric cancer
topic <i>Armillaria ostoyae</i>
liquid-cultured mycelium
ethanol extract
network pharmacology
molecular docking
gastric cancer
url https://www.mdpi.com/1420-3049/29/7/1668
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