Identification of novel flavin-dependent monooxygenase from Strobilanthes Cusia reveals molecular basis of indoles’ biosynthetic logic
Abstract Background Strobilanthes cusia (Nees) Kuntze is a traditional medical plant distributed widely in south China. The indole compounds that originated from the plant are responsible for its pharmacological activities. However, the reason why indole ingredients are accumulated in this herb and...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
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BMC
2023-10-01
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| Series: | BMC Plant Biology |
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| Online Access: | https://doi.org/10.1186/s12870-023-04557-5 |
| _version_ | 1797453323175985152 |
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| author | Chang Liu Mengya Cheng Chao Ma Junfeng Chen Hexin Tan |
| author_facet | Chang Liu Mengya Cheng Chao Ma Junfeng Chen Hexin Tan |
| author_sort | Chang Liu |
| collection | DOAJ |
| description | Abstract Background Strobilanthes cusia (Nees) Kuntze is a traditional medical plant distributed widely in south China. The indole compounds that originated from the plant are responsible for its pharmacological activities. However, the reason why indole ingredients are accumulated in this herb and how it is biosynthesized has remained largely unknown. Results In this study, metabolic and transcriptional profiling measurement experiments of different S. cusia organs were carried out to understand the underlying molecular basis of indoles’ biosynthetic logic. A metabolic investigation demonstrated that the indoles are primarily accumulated mainly in aerial parts, particularly in leaves. RNA-seq was employed to reveal the organ specific accumulation of indoles in different S. cusia organs. Meanwhile, a flavin-dependent monooxygenase gene (ScFMO1) was found in S. cusia, and it has capacity to produce indoxyl from indole by the fermentation assay. Finally, we assessed the outcomes of transient expression experiment in tobacco and confirmed that ScFMO1 localizes in cytoplasm. Conclusions Our results suggest that ScFMO1 plays a key role in biosynthesis of indoles (Indigo, indirubin, indican, etc.), it will be useful for illuminating the molecular basis of the medicinal indoles’ biosynthesis and developing strategies for improving their yields. |
| first_indexed | 2024-03-09T15:21:10Z |
| format | Article |
| id | doaj.art-2152be37c72749a29d2d9570479aac77 |
| institution | Directory Open Access Journal |
| issn | 1471-2229 |
| language | English |
| last_indexed | 2024-03-09T15:21:10Z |
| publishDate | 2023-10-01 |
| publisher | BMC |
| record_format | Article |
| series | BMC Plant Biology |
| spelling | doaj.art-2152be37c72749a29d2d9570479aac772023-11-26T12:47:10ZengBMCBMC Plant Biology1471-22292023-10-0123111210.1186/s12870-023-04557-5Identification of novel flavin-dependent monooxygenase from Strobilanthes Cusia reveals molecular basis of indoles’ biosynthetic logicChang Liu0Mengya Cheng1Chao Ma2Junfeng Chen3Hexin Tan4Department Chinese Medicine Authentication, College of Pharmacy, Naval Medical University (Second Military Medical University)Department Chinese Medicine Authentication, College of Pharmacy, Naval Medical University (Second Military Medical University)Department of Vascular Disease, Shanghai TCM-Integrated Hospital, Shanghai University of Traditional Chinese MedicineDepartment Chinese Medicine Authentication, College of Pharmacy, Naval Medical University (Second Military Medical University)Department Chinese Medicine Authentication, College of Pharmacy, Naval Medical University (Second Military Medical University)Abstract Background Strobilanthes cusia (Nees) Kuntze is a traditional medical plant distributed widely in south China. The indole compounds that originated from the plant are responsible for its pharmacological activities. However, the reason why indole ingredients are accumulated in this herb and how it is biosynthesized has remained largely unknown. Results In this study, metabolic and transcriptional profiling measurement experiments of different S. cusia organs were carried out to understand the underlying molecular basis of indoles’ biosynthetic logic. A metabolic investigation demonstrated that the indoles are primarily accumulated mainly in aerial parts, particularly in leaves. RNA-seq was employed to reveal the organ specific accumulation of indoles in different S. cusia organs. Meanwhile, a flavin-dependent monooxygenase gene (ScFMO1) was found in S. cusia, and it has capacity to produce indoxyl from indole by the fermentation assay. Finally, we assessed the outcomes of transient expression experiment in tobacco and confirmed that ScFMO1 localizes in cytoplasm. Conclusions Our results suggest that ScFMO1 plays a key role in biosynthesis of indoles (Indigo, indirubin, indican, etc.), it will be useful for illuminating the molecular basis of the medicinal indoles’ biosynthesis and developing strategies for improving their yields.https://doi.org/10.1186/s12870-023-04557-5Strobilanthes CusiaIndolesBiosynthesisScFMO |
| spellingShingle | Chang Liu Mengya Cheng Chao Ma Junfeng Chen Hexin Tan Identification of novel flavin-dependent monooxygenase from Strobilanthes Cusia reveals molecular basis of indoles’ biosynthetic logic BMC Plant Biology Strobilanthes Cusia Indoles Biosynthesis ScFMO |
| title | Identification of novel flavin-dependent monooxygenase from Strobilanthes Cusia reveals molecular basis of indoles’ biosynthetic logic |
| title_full | Identification of novel flavin-dependent monooxygenase from Strobilanthes Cusia reveals molecular basis of indoles’ biosynthetic logic |
| title_fullStr | Identification of novel flavin-dependent monooxygenase from Strobilanthes Cusia reveals molecular basis of indoles’ biosynthetic logic |
| title_full_unstemmed | Identification of novel flavin-dependent monooxygenase from Strobilanthes Cusia reveals molecular basis of indoles’ biosynthetic logic |
| title_short | Identification of novel flavin-dependent monooxygenase from Strobilanthes Cusia reveals molecular basis of indoles’ biosynthetic logic |
| title_sort | identification of novel flavin dependent monooxygenase from strobilanthes cusia reveals molecular basis of indoles biosynthetic logic |
| topic | Strobilanthes Cusia Indoles Biosynthesis ScFMO |
| url | https://doi.org/10.1186/s12870-023-04557-5 |
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