Electrolyte Leakage and the Protective Effect of Nitric Oxide on Leaves of Flooded Rice Exposed to Herbicides
ABSTRACT The nitric oxide acts on the antioxidant system of plants and can discontinue the damage of herbicides elicitors of oxidative stress that cause the disruption of membranes and leakage of cellular contents. In order to evaluate the protective effect of nitric oxide in electrolytes leakage, l...
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| Format: | Article |
| Language: | English |
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Sociedade Brasileira da Ciência das Plantas Daninhas
2016-12-01
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| Series: | Planta Daninha |
| Subjects: | |
| Online Access: | http://www.scielo.br/pdf/pd/v34n4/0100-8358-pd-34-04-00777.pdf |
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| author | K.S. SILVA L.J. K. URBAN A. BALBINOT F.S. GNOCATO N.D. KRUSE E. MARCHESAN S.L.O. MACHADO |
| author_facet | K.S. SILVA L.J. K. URBAN A. BALBINOT F.S. GNOCATO N.D. KRUSE E. MARCHESAN S.L.O. MACHADO |
| author_sort | K.S. SILVA |
| collection | DOAJ |
| description | ABSTRACT The nitric oxide acts on the antioxidant system of plants and can discontinue the damage of herbicides elicitors of oxidative stress that cause the disruption of membranes and leakage of cellular contents. In order to evaluate the protective effect of nitric oxide in electrolytes leakage, leaf segments of the Puita INTA CL rice cultivar were incubated with 0, 5, 50, 500 and 5,000 μM clomazone (360 g a.i. L-1), oxadiazon (250 g a.i. L-1), oxyfluorfen (240 g a.i. L-1) and the formulated mixture of paraquat (200 g a.i. L-1) + diuron (100 g a.i. L-1) to obtain the maximum potential conductivity of 50% (MPC50). Subsequently, leaf segments were pre-treated with 0, 200 and 2,000 μM of sodium nitroprusside (SNP) for four hours and further incubated for 48 hours with 0, 0.5, 1, 2 and 4 times the concentration of the herbicide that caused the CMP50, and the protective effect was reassessed in the presence of nitric oxide scavenger, cPTIO. The MPC50 was caused by exposure to 188.9, 273.4, 410.2 + 205.1 and 917.0 μM of Oxadiazon, Oxyfluorfen, Paraquat + Diuron and Clomazone. Pretreatment with 200 μM of SNP reduced electrolyte leakage in leaf segments exposed to 2 and 4 times the MPC50 to oxadiazon and paraquat + diuron, while 2,000 μM reduced the damage caused by oxyfluorfen, at the same concentrations. Also, 200 and 2,000 μM of SNP were efficient for clomazone, and the protection was confirmed by cPTIO in all cases. |
| first_indexed | 2024-12-19T17:16:06Z |
| format | Article |
| id | doaj.art-2155045ca0c44aa6be4cfdd74db2880a |
| institution | Directory Open Access Journal |
| issn | 0100-8358 |
| language | English |
| last_indexed | 2024-12-19T17:16:06Z |
| publishDate | 2016-12-01 |
| publisher | Sociedade Brasileira da Ciência das Plantas Daninhas |
| record_format | Article |
| series | Planta Daninha |
| spelling | doaj.art-2155045ca0c44aa6be4cfdd74db2880a2022-12-21T20:12:52ZengSociedade Brasileira da Ciência das Plantas DaninhasPlanta Daninha0100-83582016-12-0134477778610.1590/s0100-83582016340400018Electrolyte Leakage and the Protective Effect of Nitric Oxide on Leaves of Flooded Rice Exposed to HerbicidesK.S. SILVAL.J. K. URBANA. BALBINOTF.S. GNOCATON.D. KRUSEE. MARCHESANS.L.O. MACHADOABSTRACT The nitric oxide acts on the antioxidant system of plants and can discontinue the damage of herbicides elicitors of oxidative stress that cause the disruption of membranes and leakage of cellular contents. In order to evaluate the protective effect of nitric oxide in electrolytes leakage, leaf segments of the Puita INTA CL rice cultivar were incubated with 0, 5, 50, 500 and 5,000 μM clomazone (360 g a.i. L-1), oxadiazon (250 g a.i. L-1), oxyfluorfen (240 g a.i. L-1) and the formulated mixture of paraquat (200 g a.i. L-1) + diuron (100 g a.i. L-1) to obtain the maximum potential conductivity of 50% (MPC50). Subsequently, leaf segments were pre-treated with 0, 200 and 2,000 μM of sodium nitroprusside (SNP) for four hours and further incubated for 48 hours with 0, 0.5, 1, 2 and 4 times the concentration of the herbicide that caused the CMP50, and the protective effect was reassessed in the presence of nitric oxide scavenger, cPTIO. The MPC50 was caused by exposure to 188.9, 273.4, 410.2 + 205.1 and 917.0 μM of Oxadiazon, Oxyfluorfen, Paraquat + Diuron and Clomazone. Pretreatment with 200 μM of SNP reduced electrolyte leakage in leaf segments exposed to 2 and 4 times the MPC50 to oxadiazon and paraquat + diuron, while 2,000 μM reduced the damage caused by oxyfluorfen, at the same concentrations. Also, 200 and 2,000 μM of SNP were efficient for clomazone, and the protection was confirmed by cPTIO in all cases.http://www.scielo.br/pdf/pd/v34n4/0100-8358-pd-34-04-00777.pdfOryza sativasodium nitroprussideclomazoneoxadiazonoxyfluorfenparaquat + diuron |
| spellingShingle | K.S. SILVA L.J. K. URBAN A. BALBINOT F.S. GNOCATO N.D. KRUSE E. MARCHESAN S.L.O. MACHADO Electrolyte Leakage and the Protective Effect of Nitric Oxide on Leaves of Flooded Rice Exposed to Herbicides Planta Daninha Oryza sativa sodium nitroprusside clomazone oxadiazon oxyfluorfen paraquat + diuron |
| title | Electrolyte Leakage and the Protective Effect of Nitric Oxide on Leaves of Flooded Rice Exposed to Herbicides |
| title_full | Electrolyte Leakage and the Protective Effect of Nitric Oxide on Leaves of Flooded Rice Exposed to Herbicides |
| title_fullStr | Electrolyte Leakage and the Protective Effect of Nitric Oxide on Leaves of Flooded Rice Exposed to Herbicides |
| title_full_unstemmed | Electrolyte Leakage and the Protective Effect of Nitric Oxide on Leaves of Flooded Rice Exposed to Herbicides |
| title_short | Electrolyte Leakage and the Protective Effect of Nitric Oxide on Leaves of Flooded Rice Exposed to Herbicides |
| title_sort | electrolyte leakage and the protective effect of nitric oxide on leaves of flooded rice exposed to herbicides |
| topic | Oryza sativa sodium nitroprusside clomazone oxadiazon oxyfluorfen paraquat + diuron |
| url | http://www.scielo.br/pdf/pd/v34n4/0100-8358-pd-34-04-00777.pdf |
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