Yet Another Similarity between Mitochondrial and Bacterial Ribosomal Small Subunit Biogenesis Obtained by Structural Characterization of RbfA from <i>S. aureus</i>
Ribosome biogenesis is a complex and highly accurate conservative process of ribosomal subunit maturation followed by association. Subunit maturation comprises sequential stages of ribosomal RNA and proteins’ folding, modification and binding, with the involvement of numerous RNAses, helicases, GTPa...
| Main Authors: | , , , , , , , , , , , , , , |
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| Format: | Article |
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MDPI AG
2023-01-01
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| Series: | International Journal of Molecular Sciences |
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| Online Access: | https://www.mdpi.com/1422-0067/24/3/2118 |
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| author | Aydar G. Bikmullin Bulat Fatkhullin Artem Stetsenko Azat Gabdulkhakov Natalia Garaeva Liliia Nurullina Evelina Klochkova Alexander Golubev Iskander Khusainov Natalie Trachtmann Dmitriy Blokhin Albert Guskov Shamil Validov Konstantin Usachev Marat Yusupov |
| author_facet | Aydar G. Bikmullin Bulat Fatkhullin Artem Stetsenko Azat Gabdulkhakov Natalia Garaeva Liliia Nurullina Evelina Klochkova Alexander Golubev Iskander Khusainov Natalie Trachtmann Dmitriy Blokhin Albert Guskov Shamil Validov Konstantin Usachev Marat Yusupov |
| author_sort | Aydar G. Bikmullin |
| collection | DOAJ |
| description | Ribosome biogenesis is a complex and highly accurate conservative process of ribosomal subunit maturation followed by association. Subunit maturation comprises sequential stages of ribosomal RNA and proteins’ folding, modification and binding, with the involvement of numerous RNAses, helicases, GTPases, chaperones, RNA, protein-modifying enzymes, and assembly factors. One such assembly factor involved in bacterial 30S subunit maturation is ribosomal binding factor A (RbfA). In this study, we present the crystal (determined at 2.2 Å resolution) and NMR structures of RbfA as well as the 2.9 Å resolution cryo-EM reconstruction of the 30S–RbfA complex from <i>Staphylococcus aureus</i> (<i>S. aureus</i>). Additionally, we show that the manner of RbfA action on the small ribosomal subunit during its maturation is shared between bacteria and mitochondria. The obtained results clarify the function of RbfA in the 30S maturation process and its role in ribosome functioning in general. Furthermore, given that <i>S. aureus</i> is a serious human pathogen, this study provides an additional prospect to develop antimicrobials targeting bacterial pathogens. |
| first_indexed | 2024-03-11T09:42:35Z |
| format | Article |
| id | doaj.art-21559ab3cb814ca2940fa727f01e3c0d |
| institution | Directory Open Access Journal |
| issn | 1661-6596 1422-0067 |
| language | English |
| last_indexed | 2024-03-11T09:42:35Z |
| publishDate | 2023-01-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | International Journal of Molecular Sciences |
| spelling | doaj.art-21559ab3cb814ca2940fa727f01e3c0d2023-11-16T16:52:37ZengMDPI AGInternational Journal of Molecular Sciences1661-65961422-00672023-01-01243211810.3390/ijms24032118Yet Another Similarity between Mitochondrial and Bacterial Ribosomal Small Subunit Biogenesis Obtained by Structural Characterization of RbfA from <i>S. aureus</i>Aydar G. Bikmullin0Bulat Fatkhullin1Artem Stetsenko2Azat Gabdulkhakov3Natalia Garaeva4Liliia Nurullina5Evelina Klochkova6Alexander Golubev7Iskander Khusainov8Natalie Trachtmann9Dmitriy Blokhin10Albert Guskov11Shamil Validov12Konstantin Usachev13Marat Yusupov14Laboratory of Structural Biology, Institute of Fundamental Medicine and Biology, Kazan Federal University, 420021 Kazan, RussiaDepartment of Integrated Structural Biology, Institute of Genetics and Molecular and Cellular Biology, University of Strasbourg, 67400 Illkirch, FranceGroningen Biomolecular Sciences and Biotechnology Institute (GBB), University of Groningen, 9700 AB Groningen, The NetherlandsInstitute of Protein Research, Russian Academy of Sciences, 142290 Pushchino, RussiaLaboratory of Structural Biology, Institute of Fundamental Medicine and Biology, Kazan Federal University, 420021 Kazan, RussiaDepartment of Integrated Structural Biology, Institute of Genetics and Molecular and Cellular Biology, University of Strasbourg, 67400 Illkirch, FranceLaboratory of Structural Biology, Institute of Fundamental Medicine and Biology, Kazan Federal University, 420021 Kazan, RussiaLaboratory of Structural Biology, Institute of Fundamental Medicine and Biology, Kazan Federal University, 420021 Kazan, RussiaOpen Lab ‘Biomarker’, Kazan Federal University, 420008 Kazan, RussiaInstitute of Microbiology, University of Stuttgart, D-70569 Stuttgart, GermanyNMR Laboratory, Medical Physics Department, Institute of Physics, Kazan Federal University, 420008 Kazan, RussiaGroningen Biomolecular Sciences and Biotechnology Institute (GBB), University of Groningen, 9700 AB Groningen, The NetherlandsLaboratory of Structural Biology, Institute of Fundamental Medicine and Biology, Kazan Federal University, 420021 Kazan, RussiaLaboratory of Structural Biology, Institute of Fundamental Medicine and Biology, Kazan Federal University, 420021 Kazan, RussiaLaboratory of Structural Biology, Institute of Fundamental Medicine and Biology, Kazan Federal University, 420021 Kazan, RussiaRibosome biogenesis is a complex and highly accurate conservative process of ribosomal subunit maturation followed by association. Subunit maturation comprises sequential stages of ribosomal RNA and proteins’ folding, modification and binding, with the involvement of numerous RNAses, helicases, GTPases, chaperones, RNA, protein-modifying enzymes, and assembly factors. One such assembly factor involved in bacterial 30S subunit maturation is ribosomal binding factor A (RbfA). In this study, we present the crystal (determined at 2.2 Å resolution) and NMR structures of RbfA as well as the 2.9 Å resolution cryo-EM reconstruction of the 30S–RbfA complex from <i>Staphylococcus aureus</i> (<i>S. aureus</i>). Additionally, we show that the manner of RbfA action on the small ribosomal subunit during its maturation is shared between bacteria and mitochondria. The obtained results clarify the function of RbfA in the 30S maturation process and its role in ribosome functioning in general. Furthermore, given that <i>S. aureus</i> is a serious human pathogen, this study provides an additional prospect to develop antimicrobials targeting bacterial pathogens.https://www.mdpi.com/1422-0067/24/3/2118RbfA30S biogenesisribosometranslationbacterial ribosomal proteins<i>Staphylococcus aureus</i> |
| spellingShingle | Aydar G. Bikmullin Bulat Fatkhullin Artem Stetsenko Azat Gabdulkhakov Natalia Garaeva Liliia Nurullina Evelina Klochkova Alexander Golubev Iskander Khusainov Natalie Trachtmann Dmitriy Blokhin Albert Guskov Shamil Validov Konstantin Usachev Marat Yusupov Yet Another Similarity between Mitochondrial and Bacterial Ribosomal Small Subunit Biogenesis Obtained by Structural Characterization of RbfA from <i>S. aureus</i> International Journal of Molecular Sciences RbfA 30S biogenesis ribosome translation bacterial ribosomal proteins <i>Staphylococcus aureus</i> |
| title | Yet Another Similarity between Mitochondrial and Bacterial Ribosomal Small Subunit Biogenesis Obtained by Structural Characterization of RbfA from <i>S. aureus</i> |
| title_full | Yet Another Similarity between Mitochondrial and Bacterial Ribosomal Small Subunit Biogenesis Obtained by Structural Characterization of RbfA from <i>S. aureus</i> |
| title_fullStr | Yet Another Similarity between Mitochondrial and Bacterial Ribosomal Small Subunit Biogenesis Obtained by Structural Characterization of RbfA from <i>S. aureus</i> |
| title_full_unstemmed | Yet Another Similarity between Mitochondrial and Bacterial Ribosomal Small Subunit Biogenesis Obtained by Structural Characterization of RbfA from <i>S. aureus</i> |
| title_short | Yet Another Similarity between Mitochondrial and Bacterial Ribosomal Small Subunit Biogenesis Obtained by Structural Characterization of RbfA from <i>S. aureus</i> |
| title_sort | yet another similarity between mitochondrial and bacterial ribosomal small subunit biogenesis obtained by structural characterization of rbfa from i s aureus i |
| topic | RbfA 30S biogenesis ribosome translation bacterial ribosomal proteins <i>Staphylococcus aureus</i> |
| url | https://www.mdpi.com/1422-0067/24/3/2118 |
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