LIPUS inhibits inflammation and catabolism through the NF‐κB pathway in human degenerative nucleus pulposus cells
Abstract Background Low-intensity pulsed ultrasound (LIPUS) is a safe and noninvasive rehabilitative physical therapy with anti-inflammatory effects. The current study investigated the effect of LIPUS on the inflammation of nucleus pulposus (NP) cells and its underlying mechanism. Methods Human NP c...
| Main Authors: | , , , , , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
BMC
2021-10-01
|
| Series: | Journal of Orthopaedic Surgery and Research |
| Subjects: | |
| Online Access: | https://doi.org/10.1186/s13018-021-02739-1 |
| _version_ | 1798040038537166848 |
|---|---|
| author | Weiwei Yi Qing Chen Chuan Liu Kaiting Li Bailong Tao Guihua Tian Lu Zhou Xiaohong Li Jieliang Shen Bo Liu Zhenming Hu Dawu Wang Dingqun Bai |
| author_facet | Weiwei Yi Qing Chen Chuan Liu Kaiting Li Bailong Tao Guihua Tian Lu Zhou Xiaohong Li Jieliang Shen Bo Liu Zhenming Hu Dawu Wang Dingqun Bai |
| author_sort | Weiwei Yi |
| collection | DOAJ |
| description | Abstract Background Low-intensity pulsed ultrasound (LIPUS) is a safe and noninvasive rehabilitative physical therapy with anti-inflammatory effects. The current study investigated the effect of LIPUS on the inflammation of nucleus pulposus (NP) cells and its underlying mechanism. Methods Human NP cells were acquired from lumbar disc herniation tissue samples and cultured for experiments. Human NP cells were treated with LPS and then exposed to LIPUS (15 mW/cm2, 30 mW/cm2 and 60 mW/cm2) for 20 min daily for 3 days to determine the appropriate intensity to inhibit the expression of the inflammatory factors TNF-α and IL-1β. The gene and protein expression of aggrecan, collagen II, MMP-3 and MMP-9 was measured by real‐time PCR and western blotting, respectively. The activity of the nuclear factor‐kappa B (NF‐κB) pathway was examined by western blotting and immunofluorescence. After pretreatment with the NF-κB inhibitor PDTC, the expression of TNF-α, IL-1β, MMP-3 and MMP-9 was measured by real‐time PCR. Results LIPUS at intensities of 15 mW/cm2, 30 mW/cm2 and 60 mW/cm2 inhibited LPS-induced NP cell expression of the inflammatory factors TNF-α and IL-1β, especially at 30 mW/cm2. LIPUS significantly upregulated the gene and protein expression of aggrecan and collagen II and downregulated the gene and protein expression of MMP-3 and MMP-9 in LPS-induced NP cells. The NF‐κB signaling pathway was inhibited by LIPUS through inhibiting the protein expression of p-P65 and the translocation of P65 into the nucleus in LPS-induced NP cells. In addition, LIPUS had similar effects as the NF-κB inhibitor PDTC by inhibiting the NF-κB signaling pathway, inflammation and catabolism in LPS-induced human degenerative nucleus pulposus cells. Conclusion LIPUS inhibited inflammation and catabolism through the NF‐κB pathway in human degenerative nucleus pulposus cells. |
| first_indexed | 2024-04-11T22:01:59Z |
| format | Article |
| id | doaj.art-215632c9aaf940568badca018f76cb55 |
| institution | Directory Open Access Journal |
| issn | 1749-799X |
| language | English |
| last_indexed | 2024-04-11T22:01:59Z |
| publishDate | 2021-10-01 |
| publisher | BMC |
| record_format | Article |
| series | Journal of Orthopaedic Surgery and Research |
| spelling | doaj.art-215632c9aaf940568badca018f76cb552022-12-22T04:00:53ZengBMCJournal of Orthopaedic Surgery and Research1749-799X2021-10-011611910.1186/s13018-021-02739-1LIPUS inhibits inflammation and catabolism through the NF‐κB pathway in human degenerative nucleus pulposus cellsWeiwei Yi0Qing Chen1Chuan Liu2Kaiting Li3Bailong Tao4Guihua Tian5Lu Zhou6Xiaohong Li7Jieliang Shen8Bo Liu9Zhenming Hu10Dawu Wang11Dingqun Bai12Department of Rehabilitation Medicine, The First Affiliated Hospital of Chongqing Medical UniversityDepartment of Rehabilitation Medicine, The First Affiliated Hospital of Chongqing Medical UniversityLaboratory Research Center, The First Affiliated Hospital of Chongqing Medical UniversityDepartment of Rehabilitation Medicine, The First Affiliated Hospital of Chongqing Medical UniversityDepartment of Radiology, The First Affiliated Hospital of Chongqing Medical UniversityDepartment of Rehabilitation Medicine, The First Affiliated Hospital of Chongqing Medical UniversityDepartment of Rehabilitation Medicine, The First Affiliated Hospital of Chongqing Medical UniversityDepartment of Rehabilitation Medicine, The First Affiliated Hospital of Chongqing Medical UniversityDepartment of Orthopedics, The First Affiliated Hospital of Chongqing Medical UniversityDepartment of Orthopedics, The First Affiliated Hospital of Chongqing Medical UniversityDepartment of Orthopedics, The First Affiliated Hospital of Chongqing Medical UniversityDepartment of Rehabilitation Medicine, The First Affiliated Hospital of Chongqing Medical UniversityDepartment of Rehabilitation Medicine, The First Affiliated Hospital of Chongqing Medical UniversityAbstract Background Low-intensity pulsed ultrasound (LIPUS) is a safe and noninvasive rehabilitative physical therapy with anti-inflammatory effects. The current study investigated the effect of LIPUS on the inflammation of nucleus pulposus (NP) cells and its underlying mechanism. Methods Human NP cells were acquired from lumbar disc herniation tissue samples and cultured for experiments. Human NP cells were treated with LPS and then exposed to LIPUS (15 mW/cm2, 30 mW/cm2 and 60 mW/cm2) for 20 min daily for 3 days to determine the appropriate intensity to inhibit the expression of the inflammatory factors TNF-α and IL-1β. The gene and protein expression of aggrecan, collagen II, MMP-3 and MMP-9 was measured by real‐time PCR and western blotting, respectively. The activity of the nuclear factor‐kappa B (NF‐κB) pathway was examined by western blotting and immunofluorescence. After pretreatment with the NF-κB inhibitor PDTC, the expression of TNF-α, IL-1β, MMP-3 and MMP-9 was measured by real‐time PCR. Results LIPUS at intensities of 15 mW/cm2, 30 mW/cm2 and 60 mW/cm2 inhibited LPS-induced NP cell expression of the inflammatory factors TNF-α and IL-1β, especially at 30 mW/cm2. LIPUS significantly upregulated the gene and protein expression of aggrecan and collagen II and downregulated the gene and protein expression of MMP-3 and MMP-9 in LPS-induced NP cells. The NF‐κB signaling pathway was inhibited by LIPUS through inhibiting the protein expression of p-P65 and the translocation of P65 into the nucleus in LPS-induced NP cells. In addition, LIPUS had similar effects as the NF-κB inhibitor PDTC by inhibiting the NF-κB signaling pathway, inflammation and catabolism in LPS-induced human degenerative nucleus pulposus cells. Conclusion LIPUS inhibited inflammation and catabolism through the NF‐κB pathway in human degenerative nucleus pulposus cells.https://doi.org/10.1186/s13018-021-02739-1Low-intensity pulsed ultrasoundNF-κBInflammationNucleus pulposus cells |
| spellingShingle | Weiwei Yi Qing Chen Chuan Liu Kaiting Li Bailong Tao Guihua Tian Lu Zhou Xiaohong Li Jieliang Shen Bo Liu Zhenming Hu Dawu Wang Dingqun Bai LIPUS inhibits inflammation and catabolism through the NF‐κB pathway in human degenerative nucleus pulposus cells Journal of Orthopaedic Surgery and Research Low-intensity pulsed ultrasound NF-κB Inflammation Nucleus pulposus cells |
| title | LIPUS inhibits inflammation and catabolism through the NF‐κB pathway in human degenerative nucleus pulposus cells |
| title_full | LIPUS inhibits inflammation and catabolism through the NF‐κB pathway in human degenerative nucleus pulposus cells |
| title_fullStr | LIPUS inhibits inflammation and catabolism through the NF‐κB pathway in human degenerative nucleus pulposus cells |
| title_full_unstemmed | LIPUS inhibits inflammation and catabolism through the NF‐κB pathway in human degenerative nucleus pulposus cells |
| title_short | LIPUS inhibits inflammation and catabolism through the NF‐κB pathway in human degenerative nucleus pulposus cells |
| title_sort | lipus inhibits inflammation and catabolism through the nf κb pathway in human degenerative nucleus pulposus cells |
| topic | Low-intensity pulsed ultrasound NF-κB Inflammation Nucleus pulposus cells |
| url | https://doi.org/10.1186/s13018-021-02739-1 |
| work_keys_str_mv | AT weiweiyi lipusinhibitsinflammationandcatabolismthroughthenfkbpathwayinhumandegenerativenucleuspulposuscells AT qingchen lipusinhibitsinflammationandcatabolismthroughthenfkbpathwayinhumandegenerativenucleuspulposuscells AT chuanliu lipusinhibitsinflammationandcatabolismthroughthenfkbpathwayinhumandegenerativenucleuspulposuscells AT kaitingli lipusinhibitsinflammationandcatabolismthroughthenfkbpathwayinhumandegenerativenucleuspulposuscells AT bailongtao lipusinhibitsinflammationandcatabolismthroughthenfkbpathwayinhumandegenerativenucleuspulposuscells AT guihuatian lipusinhibitsinflammationandcatabolismthroughthenfkbpathwayinhumandegenerativenucleuspulposuscells AT luzhou lipusinhibitsinflammationandcatabolismthroughthenfkbpathwayinhumandegenerativenucleuspulposuscells AT xiaohongli lipusinhibitsinflammationandcatabolismthroughthenfkbpathwayinhumandegenerativenucleuspulposuscells AT jieliangshen lipusinhibitsinflammationandcatabolismthroughthenfkbpathwayinhumandegenerativenucleuspulposuscells AT boliu lipusinhibitsinflammationandcatabolismthroughthenfkbpathwayinhumandegenerativenucleuspulposuscells AT zhenminghu lipusinhibitsinflammationandcatabolismthroughthenfkbpathwayinhumandegenerativenucleuspulposuscells AT dawuwang lipusinhibitsinflammationandcatabolismthroughthenfkbpathwayinhumandegenerativenucleuspulposuscells AT dingqunbai lipusinhibitsinflammationandcatabolismthroughthenfkbpathwayinhumandegenerativenucleuspulposuscells |