Phenolic Compounds from Moroccan <i>Retama monosperma</i> L. Boiss, <i>Berberis vulgaris</i> L. and <i>Ricinus communis</i> L.: Characterization, Antioxidant Activity and Performance Criteria of the Validated Method Using UHPLC/DAD/ESI-MS
Components of medicinal plants have many pharmacological activities, including antioxidant activity, playing an important role in limiting oxidative stress that can cause several damages. This paper characterizes polyphenols of Retama monosperma L., Berberis vulgaris L. and Ricinus communis L. plant...
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Format: | Article |
Language: | English |
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Department of Chemistry, Universitas Gadjah Mada
2023-12-01
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Series: | Indonesian Journal of Chemistry |
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Online Access: | https://jurnal.ugm.ac.id/ijc/article/view/87157 |
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author | Meriem Outaki Manal Zefzoufi Amal Sammama Khadija El Gadali Rabiaa Fdil Layla El Gaini |
author_facet | Meriem Outaki Manal Zefzoufi Amal Sammama Khadija El Gadali Rabiaa Fdil Layla El Gaini |
author_sort | Meriem Outaki |
collection | DOAJ |
description | Components of medicinal plants have many pharmacological activities, including antioxidant activity, playing an important role in limiting oxidative stress that can cause several damages. This paper characterizes polyphenols of Retama monosperma L., Berberis vulgaris L. and Ricinus communis L. plant extracts and evaluates their antioxidant activity by DPPH, conjugated diene and TBARS assay. To ensure the quality of analytical results, this paper presents performance criteria of the validated method using UHPLC/DAD/ESI-MS. Regarding method validation, the results confirm different used tests and evaluate detection and quantification limits. Concerning the characterization and study of antioxidant activity, realized testing showed that R. monosperma is rich in isoflavone, flavone and flavonol. For R. communis, we notice the presence of rutin as a major compound. Meanwhile, B. vulgaris contains significant amounts of gallic acid and p-coumaric acid. These plant extracts have high antioxidant activity due to the presence of phenolic compounds. |
first_indexed | 2024-03-08T08:53:02Z |
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issn | 1411-9420 2460-1578 |
language | English |
last_indexed | 2024-03-08T08:53:02Z |
publishDate | 2023-12-01 |
publisher | Department of Chemistry, Universitas Gadjah Mada |
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series | Indonesian Journal of Chemistry |
spelling | doaj.art-21587d0800944f34bcbe655e1b57a5cf2024-02-01T07:39:03ZengDepartment of Chemistry, Universitas Gadjah MadaIndonesian Journal of Chemistry1411-94202460-15782023-12-012361704172110.22146/ijc.8715734828Phenolic Compounds from Moroccan <i>Retama monosperma</i> L. Boiss, <i>Berberis vulgaris</i> L. and <i>Ricinus communis</i> L.: Characterization, Antioxidant Activity and Performance Criteria of the Validated Method Using UHPLC/DAD/ESI-MSMeriem Outaki0Manal Zefzoufi1Amal Sammama2Khadija El Gadali3Rabiaa Fdil4Layla El Gaini5Laboratory of Applied Chemistry and Environment, Faculty of Sciences and Technologies, University Hassan I, PC. 577, Settat, MoroccoLaboratory of Bioorganic Chemistry, Department of Chemistry, Faculty of Sciences, University Chouaïb Doukkali, PC. 20, El Jadida, MoroccoCenter of Analysis and Characterization, University Cadi Ayyad, PC. 511, Marrakech, MoroccoLaboratory of Sustainable Development and Health Research, Faculty of Sciences and Technologies, University Cadi Ayyad, PC. 549, Marrakech, MoroccoLaboratory of Bioorganic Chemistry, Department of Chemistry, Faculty of Sciences, University Chouaïb Doukkali, PC. 20, El Jadida, MoroccoCenter of Analysis and Characterization, University Cadi Ayyad, PC. 511, Marrakech, Morocco; Laboratory of Applied Chemistry and Biomass, Department of Chemistry, Faculty of Sciences Semlalia, University Cadi Ayyad, PC. 2390, Marrakech, MoroccoComponents of medicinal plants have many pharmacological activities, including antioxidant activity, playing an important role in limiting oxidative stress that can cause several damages. This paper characterizes polyphenols of Retama monosperma L., Berberis vulgaris L. and Ricinus communis L. plant extracts and evaluates their antioxidant activity by DPPH, conjugated diene and TBARS assay. To ensure the quality of analytical results, this paper presents performance criteria of the validated method using UHPLC/DAD/ESI-MS. Regarding method validation, the results confirm different used tests and evaluate detection and quantification limits. Concerning the characterization and study of antioxidant activity, realized testing showed that R. monosperma is rich in isoflavone, flavone and flavonol. For R. communis, we notice the presence of rutin as a major compound. Meanwhile, B. vulgaris contains significant amounts of gallic acid and p-coumaric acid. These plant extracts have high antioxidant activity due to the presence of phenolic compounds.https://jurnal.ugm.ac.id/ijc/article/view/87157polyphenolantioxidant activitymethod validationuhplc/dad/esi-ms |
spellingShingle | Meriem Outaki Manal Zefzoufi Amal Sammama Khadija El Gadali Rabiaa Fdil Layla El Gaini Phenolic Compounds from Moroccan <i>Retama monosperma</i> L. Boiss, <i>Berberis vulgaris</i> L. and <i>Ricinus communis</i> L.: Characterization, Antioxidant Activity and Performance Criteria of the Validated Method Using UHPLC/DAD/ESI-MS Indonesian Journal of Chemistry polyphenol antioxidant activity method validation uhplc/dad/esi-ms |
title | Phenolic Compounds from Moroccan <i>Retama monosperma</i> L. Boiss, <i>Berberis vulgaris</i> L. and <i>Ricinus communis</i> L.: Characterization, Antioxidant Activity and Performance Criteria of the Validated Method Using UHPLC/DAD/ESI-MS |
title_full | Phenolic Compounds from Moroccan <i>Retama monosperma</i> L. Boiss, <i>Berberis vulgaris</i> L. and <i>Ricinus communis</i> L.: Characterization, Antioxidant Activity and Performance Criteria of the Validated Method Using UHPLC/DAD/ESI-MS |
title_fullStr | Phenolic Compounds from Moroccan <i>Retama monosperma</i> L. Boiss, <i>Berberis vulgaris</i> L. and <i>Ricinus communis</i> L.: Characterization, Antioxidant Activity and Performance Criteria of the Validated Method Using UHPLC/DAD/ESI-MS |
title_full_unstemmed | Phenolic Compounds from Moroccan <i>Retama monosperma</i> L. Boiss, <i>Berberis vulgaris</i> L. and <i>Ricinus communis</i> L.: Characterization, Antioxidant Activity and Performance Criteria of the Validated Method Using UHPLC/DAD/ESI-MS |
title_short | Phenolic Compounds from Moroccan <i>Retama monosperma</i> L. Boiss, <i>Berberis vulgaris</i> L. and <i>Ricinus communis</i> L.: Characterization, Antioxidant Activity and Performance Criteria of the Validated Method Using UHPLC/DAD/ESI-MS |
title_sort | phenolic compounds from moroccan i retama monosperma i l boiss i berberis vulgaris i l and i ricinus communis i l characterization antioxidant activity and performance criteria of the validated method using uhplc dad esi ms |
topic | polyphenol antioxidant activity method validation uhplc/dad/esi-ms |
url | https://jurnal.ugm.ac.id/ijc/article/view/87157 |
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