Phenolic Compounds from Moroccan <i>Retama monosperma</i> L. Boiss, <i>Berberis vulgaris</i> L. and <i>Ricinus communis</i> L.: Characterization, Antioxidant Activity and Performance Criteria of the Validated Method Using UHPLC/DAD/ESI-MS

Components of medicinal plants have many pharmacological activities, including antioxidant activity, playing an important role in limiting oxidative stress that can cause several damages. This paper characterizes polyphenols of Retama monosperma L., Berberis vulgaris L. and Ricinus communis L. plant...

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Main Authors: Meriem Outaki, Manal Zefzoufi, Amal Sammama, Khadija El Gadali, Rabiaa Fdil, Layla El Gaini
Format: Article
Language:English
Published: Department of Chemistry, Universitas Gadjah Mada 2023-12-01
Series:Indonesian Journal of Chemistry
Subjects:
Online Access:https://jurnal.ugm.ac.id/ijc/article/view/87157
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author Meriem Outaki
Manal Zefzoufi
Amal Sammama
Khadija El Gadali
Rabiaa Fdil
Layla El Gaini
author_facet Meriem Outaki
Manal Zefzoufi
Amal Sammama
Khadija El Gadali
Rabiaa Fdil
Layla El Gaini
author_sort Meriem Outaki
collection DOAJ
description Components of medicinal plants have many pharmacological activities, including antioxidant activity, playing an important role in limiting oxidative stress that can cause several damages. This paper characterizes polyphenols of Retama monosperma L., Berberis vulgaris L. and Ricinus communis L. plant extracts and evaluates their antioxidant activity by DPPH, conjugated diene and TBARS assay. To ensure the quality of analytical results, this paper presents performance criteria of the validated method using UHPLC/DAD/ESI-MS. Regarding method validation, the results confirm different used tests and evaluate detection and quantification limits. Concerning the characterization and study of antioxidant activity, realized testing showed that R. monosperma is rich in isoflavone, flavone and flavonol. For R. communis, we notice the presence of rutin as a major compound. Meanwhile, B. vulgaris contains significant amounts of gallic acid and p-coumaric acid. These plant extracts have high antioxidant activity due to the presence of phenolic compounds.
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spelling doaj.art-21587d0800944f34bcbe655e1b57a5cf2024-02-01T07:39:03ZengDepartment of Chemistry, Universitas Gadjah MadaIndonesian Journal of Chemistry1411-94202460-15782023-12-012361704172110.22146/ijc.8715734828Phenolic Compounds from Moroccan <i>Retama monosperma</i> L. Boiss, <i>Berberis vulgaris</i> L. and <i>Ricinus communis</i> L.: Characterization, Antioxidant Activity and Performance Criteria of the Validated Method Using UHPLC/DAD/ESI-MSMeriem Outaki0Manal Zefzoufi1Amal Sammama2Khadija El Gadali3Rabiaa Fdil4Layla El Gaini5Laboratory of Applied Chemistry and Environment, Faculty of Sciences and Technologies, University Hassan I, PC. 577, Settat, MoroccoLaboratory of Bioorganic Chemistry, Department of Chemistry, Faculty of Sciences, University Chouaïb Doukkali, PC. 20, El Jadida, MoroccoCenter of Analysis and Characterization, University Cadi Ayyad, PC. 511, Marrakech, MoroccoLaboratory of Sustainable Development and Health Research, Faculty of Sciences and Technologies, University Cadi Ayyad, PC. 549, Marrakech, MoroccoLaboratory of Bioorganic Chemistry, Department of Chemistry, Faculty of Sciences, University Chouaïb Doukkali, PC. 20, El Jadida, MoroccoCenter of Analysis and Characterization, University Cadi Ayyad, PC. 511, Marrakech, Morocco; Laboratory of Applied Chemistry and Biomass, Department of Chemistry, Faculty of Sciences Semlalia, University Cadi Ayyad, PC. 2390, Marrakech, MoroccoComponents of medicinal plants have many pharmacological activities, including antioxidant activity, playing an important role in limiting oxidative stress that can cause several damages. This paper characterizes polyphenols of Retama monosperma L., Berberis vulgaris L. and Ricinus communis L. plant extracts and evaluates their antioxidant activity by DPPH, conjugated diene and TBARS assay. To ensure the quality of analytical results, this paper presents performance criteria of the validated method using UHPLC/DAD/ESI-MS. Regarding method validation, the results confirm different used tests and evaluate detection and quantification limits. Concerning the characterization and study of antioxidant activity, realized testing showed that R. monosperma is rich in isoflavone, flavone and flavonol. For R. communis, we notice the presence of rutin as a major compound. Meanwhile, B. vulgaris contains significant amounts of gallic acid and p-coumaric acid. These plant extracts have high antioxidant activity due to the presence of phenolic compounds.https://jurnal.ugm.ac.id/ijc/article/view/87157polyphenolantioxidant activitymethod validationuhplc/dad/esi-ms
spellingShingle Meriem Outaki
Manal Zefzoufi
Amal Sammama
Khadija El Gadali
Rabiaa Fdil
Layla El Gaini
Phenolic Compounds from Moroccan <i>Retama monosperma</i> L. Boiss, <i>Berberis vulgaris</i> L. and <i>Ricinus communis</i> L.: Characterization, Antioxidant Activity and Performance Criteria of the Validated Method Using UHPLC/DAD/ESI-MS
Indonesian Journal of Chemistry
polyphenol
antioxidant activity
method validation
uhplc/dad/esi-ms
title Phenolic Compounds from Moroccan <i>Retama monosperma</i> L. Boiss, <i>Berberis vulgaris</i> L. and <i>Ricinus communis</i> L.: Characterization, Antioxidant Activity and Performance Criteria of the Validated Method Using UHPLC/DAD/ESI-MS
title_full Phenolic Compounds from Moroccan <i>Retama monosperma</i> L. Boiss, <i>Berberis vulgaris</i> L. and <i>Ricinus communis</i> L.: Characterization, Antioxidant Activity and Performance Criteria of the Validated Method Using UHPLC/DAD/ESI-MS
title_fullStr Phenolic Compounds from Moroccan <i>Retama monosperma</i> L. Boiss, <i>Berberis vulgaris</i> L. and <i>Ricinus communis</i> L.: Characterization, Antioxidant Activity and Performance Criteria of the Validated Method Using UHPLC/DAD/ESI-MS
title_full_unstemmed Phenolic Compounds from Moroccan <i>Retama monosperma</i> L. Boiss, <i>Berberis vulgaris</i> L. and <i>Ricinus communis</i> L.: Characterization, Antioxidant Activity and Performance Criteria of the Validated Method Using UHPLC/DAD/ESI-MS
title_short Phenolic Compounds from Moroccan <i>Retama monosperma</i> L. Boiss, <i>Berberis vulgaris</i> L. and <i>Ricinus communis</i> L.: Characterization, Antioxidant Activity and Performance Criteria of the Validated Method Using UHPLC/DAD/ESI-MS
title_sort phenolic compounds from moroccan i retama monosperma i l boiss i berberis vulgaris i l and i ricinus communis i l characterization antioxidant activity and performance criteria of the validated method using uhplc dad esi ms
topic polyphenol
antioxidant activity
method validation
uhplc/dad/esi-ms
url https://jurnal.ugm.ac.id/ijc/article/view/87157
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