Study on the protective effects of danshen-honghua herb pair (DHHP) on myocardial ischaemia/reperfusion injury (MIRI) and potential mechanisms based on apoptosis and mitochondria
Context Danshen, the dried root and rhizome of Salvia miltiorrhiza Bunge (Labiatae) and honghua, the dried flower of Carthamus tinctorius L. (Compositae) as the herb pair was used to treat cardiovascular diseases (CVD). Objective To study the effects of DHHP on MIRI and mechanisms based on apoptosis...
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| Format: | Article |
| Language: | English |
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Taylor & Francis Group
2021-01-01
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| Series: | Pharmaceutical Biology |
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| Online Access: | http://dx.doi.org/10.1080/13880209.2021.1893346 |
| _version_ | 1818332516095885312 |
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| author | Jiqing Bai Xiaoping Wang Shaobing Du Pengfei Wang Yaheng Wang Lina Quan Yundong Xie |
| author_facet | Jiqing Bai Xiaoping Wang Shaobing Du Pengfei Wang Yaheng Wang Lina Quan Yundong Xie |
| author_sort | Jiqing Bai |
| collection | DOAJ |
| description | Context Danshen, the dried root and rhizome of Salvia miltiorrhiza Bunge (Labiatae) and honghua, the dried flower of Carthamus tinctorius L. (Compositae) as the herb pair was used to treat cardiovascular diseases (CVD). Objective To study the effects of DHHP on MIRI and mechanisms based on apoptosis and mitochondria. Materials and Methods 36 SD rats (n = 6) were randomly divided into control group (Con), the ischaemia-reperfusion group (IR), positive control (Xinning tablets, XNT, 1 g/kg/d) and DHHP (1.2, 2.4, and 4.8 g/kg/d). Except for Con, the other groups were intragastrically administrated for 5 d, the rat hearts were isolated to establish the MIRI model in vitro for evaluating the effects of DHHP on MIRI. 24 SD rats (n = 6) were randomly divided into Con, IR, DPPH2.4 (2.4 g/kg/d) and DPPH 2.4 + Atractyloside (ATR) (2.4 + 5 mg/kg/d), administered intragastrically for 5 d, then treated with ATR (5 mg/kg/d) by intraperitoneal injection in DPPH2.4 + ATR group, took rat hearts to establish MIRI model in vitro for revealing mechanism. Results Myocardial infarct sizes were, respectively, 0.35%, 40.09%, 15.84%, 30.13%, concentrations of NAD+ (nmol/gw/w) were 144, 83, 119, and 88, respectively, in Con, IR, DHHP2.4, DHHP2.4 + ATR group. Cleaved caspase-3 were 0.3, 1.6, 0.5 and 1.3% and cleaved caspase-9 were 0.2, 1.1, 0.4 and 0.8%, respectively, in Con, IR, DHHP2.4 and DHHP2.4 + ATR group. The beneficial effects of DHHP on MIRI were reversed by ATR. Conclusions The improvement of MIRI by DHHP may be involved in inhibiting MPTP opening, decreasing oxidative damage, alleviating ischaemic injury and inhibiting cardiomyocyte apoptosis. |
| first_indexed | 2024-12-13T13:36:59Z |
| format | Article |
| id | doaj.art-2159935793474d7690120e5f946b4fb2 |
| institution | Directory Open Access Journal |
| issn | 1388-0209 1744-5116 |
| language | English |
| last_indexed | 2024-12-13T13:36:59Z |
| publishDate | 2021-01-01 |
| publisher | Taylor & Francis Group |
| record_format | Article |
| series | Pharmaceutical Biology |
| spelling | doaj.art-2159935793474d7690120e5f946b4fb22022-12-21T23:43:44ZengTaylor & Francis GroupPharmaceutical Biology1388-02091744-51162021-01-0159133534610.1080/13880209.2021.18933461893346Study on the protective effects of danshen-honghua herb pair (DHHP) on myocardial ischaemia/reperfusion injury (MIRI) and potential mechanisms based on apoptosis and mitochondriaJiqing Bai0Xiaoping Wang1Shaobing Du2Pengfei Wang3Yaheng Wang4Lina Quan5Yundong Xie6College of Pharmacy, Shaanxi University of Chinese MedicineCollege of Pharmacy, Shaanxi University of Chinese MedicineCollege of Pharmacy, Shaanxi University of Chinese MedicineCollege of Pharmacy, Shaanxi University of Chinese MedicineCollege of Pharmacy, Shaanxi University of Chinese MedicineCollege of Pharmacy, Shaanxi University of Chinese MedicineCollege of Pharmacy, Shaanxi University of Chinese MedicineContext Danshen, the dried root and rhizome of Salvia miltiorrhiza Bunge (Labiatae) and honghua, the dried flower of Carthamus tinctorius L. (Compositae) as the herb pair was used to treat cardiovascular diseases (CVD). Objective To study the effects of DHHP on MIRI and mechanisms based on apoptosis and mitochondria. Materials and Methods 36 SD rats (n = 6) were randomly divided into control group (Con), the ischaemia-reperfusion group (IR), positive control (Xinning tablets, XNT, 1 g/kg/d) and DHHP (1.2, 2.4, and 4.8 g/kg/d). Except for Con, the other groups were intragastrically administrated for 5 d, the rat hearts were isolated to establish the MIRI model in vitro for evaluating the effects of DHHP on MIRI. 24 SD rats (n = 6) were randomly divided into Con, IR, DPPH2.4 (2.4 g/kg/d) and DPPH 2.4 + Atractyloside (ATR) (2.4 + 5 mg/kg/d), administered intragastrically for 5 d, then treated with ATR (5 mg/kg/d) by intraperitoneal injection in DPPH2.4 + ATR group, took rat hearts to establish MIRI model in vitro for revealing mechanism. Results Myocardial infarct sizes were, respectively, 0.35%, 40.09%, 15.84%, 30.13%, concentrations of NAD+ (nmol/gw/w) were 144, 83, 119, and 88, respectively, in Con, IR, DHHP2.4, DHHP2.4 + ATR group. Cleaved caspase-3 were 0.3, 1.6, 0.5 and 1.3% and cleaved caspase-9 were 0.2, 1.1, 0.4 and 0.8%, respectively, in Con, IR, DHHP2.4 and DHHP2.4 + ATR group. The beneficial effects of DHHP on MIRI were reversed by ATR. Conclusions The improvement of MIRI by DHHP may be involved in inhibiting MPTP opening, decreasing oxidative damage, alleviating ischaemic injury and inhibiting cardiomyocyte apoptosis.http://dx.doi.org/10.1080/13880209.2021.1893346extracts of chinese medicinecardiomyocyte apoptosismyocardial injurymyocardial mitochondria |
| spellingShingle | Jiqing Bai Xiaoping Wang Shaobing Du Pengfei Wang Yaheng Wang Lina Quan Yundong Xie Study on the protective effects of danshen-honghua herb pair (DHHP) on myocardial ischaemia/reperfusion injury (MIRI) and potential mechanisms based on apoptosis and mitochondria Pharmaceutical Biology extracts of chinese medicine cardiomyocyte apoptosis myocardial injury myocardial mitochondria |
| title | Study on the protective effects of danshen-honghua herb pair (DHHP) on myocardial ischaemia/reperfusion injury (MIRI) and potential mechanisms based on apoptosis and mitochondria |
| title_full | Study on the protective effects of danshen-honghua herb pair (DHHP) on myocardial ischaemia/reperfusion injury (MIRI) and potential mechanisms based on apoptosis and mitochondria |
| title_fullStr | Study on the protective effects of danshen-honghua herb pair (DHHP) on myocardial ischaemia/reperfusion injury (MIRI) and potential mechanisms based on apoptosis and mitochondria |
| title_full_unstemmed | Study on the protective effects of danshen-honghua herb pair (DHHP) on myocardial ischaemia/reperfusion injury (MIRI) and potential mechanisms based on apoptosis and mitochondria |
| title_short | Study on the protective effects of danshen-honghua herb pair (DHHP) on myocardial ischaemia/reperfusion injury (MIRI) and potential mechanisms based on apoptosis and mitochondria |
| title_sort | study on the protective effects of danshen honghua herb pair dhhp on myocardial ischaemia reperfusion injury miri and potential mechanisms based on apoptosis and mitochondria |
| topic | extracts of chinese medicine cardiomyocyte apoptosis myocardial injury myocardial mitochondria |
| url | http://dx.doi.org/10.1080/13880209.2021.1893346 |
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