Augmented COlorimetric NANoplasmonic (CONAN) Method for Grading Purity and Determine Concentration of EV Microliter Volume Solutions

Augmented COlorimetric NANoplasmonic (CONAN) Method for Grading Purity and Determine Concentration of EV Microliter Volume Solutions

This protocol paper describes how to assign a purity grade and to subsequently titrate extracellular vesicle (EV) solutions of a few microliters in volume by microplate COlorimetric NANoplasmonic (CONAN) assay. The CONAN assay consists of a solution of gold nanoparticles (AuNPs) into which the EV pr...

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Bibliographic Details
Main Authors: Andrea Zendrini, Lucia Paolini, Sara Busatto, Annalisa Radeghieri, Miriam Romano, Marca H. M. Wauben, Martijn J. C. van Herwijnen, Peter Nejsum, Anne Borup, Andrea Ridolfi, Costanza Montis, Paolo Bergese
Format: Article
Language:English
Published: Frontiers Media S.A. 2020-02-01
Series:Frontiers in Bioengineering and Biotechnology
Subjects:
extracellular vesicles
synthetic vesicles
liposomes
purity
titration
particle number
Online Access:https://www.frontiersin.org/article/10.3389/fbioe.2019.00452/full

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