Exploring the Anticancer Activity of Grape Seed Extract on Skin Cancer Cell Lines A431
In this study, grape seeds were extracted using ethyl acetate and petroleum ether by solvent-solvent extraction method. The phytochemical tests were performed to identify different phytochemical compounds present in the grape seed extract (GSE). Antibacterial activity of the GSE was determined using...
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| Format: | Article |
| Language: | English |
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Instituto de Tecnologia do Paraná (Tecpar)
2015-08-01
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| Series: | Brazilian Archives of Biology and Technology |
| Subjects: | |
| Online Access: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132015000400540&lng=en&tlng=en |
| _version_ | 1819268085155102720 |
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| author | V. Mohansrinivasan Subathra Devi C. Meenakshi Deori Ananya Biswas Jemimah Naine S |
| author_facet | V. Mohansrinivasan Subathra Devi C. Meenakshi Deori Ananya Biswas Jemimah Naine S |
| author_sort | V. Mohansrinivasan |
| collection | DOAJ |
| description | In this study, grape seeds were extracted using ethyl acetate and petroleum ether by solvent-solvent extraction method. The phytochemical tests were performed to identify different phytochemical compounds present in the grape seed extract (GSE). Antibacterial activity of the GSE was determined using agar diffusion method against Gram- positive and Gram-negative bacteria. Gas chromatography-mass spectrometry (GC-MS) and Fourier transform infrared spectroscopy (FTIR) analysis was done to identify the presence of bioactive compounds and their functional groups. The GC-MS results revealed a total of four compounds, known to have potent activity against cancer cells, viz, squalene, the most potent compound found in ethyl acetate extract and diethyl phthalate, ethyl-9- cis -11- trans octadecadienoate and (R)-(-)-14,-methyl-8-Hexadecyn-1-ol in petroleum ether extract. Cytotoxic activity of the GSE was observed against skin cancer cell lines A4321 using 3-(4, 5-dimethylthiazol-2-yl)-2-5-diphenyl tetrazolium bromide) MTT assay. The IC50 value of the GSE against A431 skin cancer cell line was 480 µg/mL. This is first such report against A4321 cell lines. The study gives the overall perception about importance of GSE in medicine and nutraceuticals purposes. |
| first_indexed | 2024-12-23T21:27:27Z |
| format | Article |
| id | doaj.art-215c2a2f39e94fa48ac6c36972e08dba |
| institution | Directory Open Access Journal |
| issn | 1678-4324 |
| language | English |
| last_indexed | 2024-12-23T21:27:27Z |
| publishDate | 2015-08-01 |
| publisher | Instituto de Tecnologia do Paraná (Tecpar) |
| record_format | Article |
| series | Brazilian Archives of Biology and Technology |
| spelling | doaj.art-215c2a2f39e94fa48ac6c36972e08dba2022-12-21T17:30:34ZengInstituto de Tecnologia do Paraná (Tecpar)Brazilian Archives of Biology and Technology1678-43242015-08-0158454054610.1590/s1516-8913201500076S1516-89132015000400540Exploring the Anticancer Activity of Grape Seed Extract on Skin Cancer Cell Lines A431V. MohansrinivasanSubathra Devi C.Meenakshi DeoriAnanya BiswasJemimah Naine SIn this study, grape seeds were extracted using ethyl acetate and petroleum ether by solvent-solvent extraction method. The phytochemical tests were performed to identify different phytochemical compounds present in the grape seed extract (GSE). Antibacterial activity of the GSE was determined using agar diffusion method against Gram- positive and Gram-negative bacteria. Gas chromatography-mass spectrometry (GC-MS) and Fourier transform infrared spectroscopy (FTIR) analysis was done to identify the presence of bioactive compounds and their functional groups. The GC-MS results revealed a total of four compounds, known to have potent activity against cancer cells, viz, squalene, the most potent compound found in ethyl acetate extract and diethyl phthalate, ethyl-9- cis -11- trans octadecadienoate and (R)-(-)-14,-methyl-8-Hexadecyn-1-ol in petroleum ether extract. Cytotoxic activity of the GSE was observed against skin cancer cell lines A4321 using 3-(4, 5-dimethylthiazol-2-yl)-2-5-diphenyl tetrazolium bromide) MTT assay. The IC50 value of the GSE against A431 skin cancer cell line was 480 µg/mL. This is first such report against A4321 cell lines. The study gives the overall perception about importance of GSE in medicine and nutraceuticals purposes.http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132015000400540&lng=en&tlng=enGSE (Grape seed extract)phytochemicalFTIRGCMSskin cancer cell lineanticancer agents |
| spellingShingle | V. Mohansrinivasan Subathra Devi C. Meenakshi Deori Ananya Biswas Jemimah Naine S Exploring the Anticancer Activity of Grape Seed Extract on Skin Cancer Cell Lines A431 Brazilian Archives of Biology and Technology GSE (Grape seed extract) phytochemical FTIR GCMS skin cancer cell line anticancer agents |
| title | Exploring the Anticancer Activity of Grape Seed Extract on Skin Cancer Cell Lines A431 |
| title_full | Exploring the Anticancer Activity of Grape Seed Extract on Skin Cancer Cell Lines A431 |
| title_fullStr | Exploring the Anticancer Activity of Grape Seed Extract on Skin Cancer Cell Lines A431 |
| title_full_unstemmed | Exploring the Anticancer Activity of Grape Seed Extract on Skin Cancer Cell Lines A431 |
| title_short | Exploring the Anticancer Activity of Grape Seed Extract on Skin Cancer Cell Lines A431 |
| title_sort | exploring the anticancer activity of grape seed extract on skin cancer cell lines a431 |
| topic | GSE (Grape seed extract) phytochemical FTIR GCMS skin cancer cell line anticancer agents |
| url | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132015000400540&lng=en&tlng=en |
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